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[181623_at]

Description ZK973.6 /REP_DB=WormBase Gene ID /WP=CE24752 /TR=Q9N4M4 /GB=AAF40010.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 35      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 02      6grp_total > 25      4grp_total > 46      6grp_highest_sub > 01_02      6grp_cgc > 24      6grp_high1_sub > 02      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2121
1 2 3 4 range
A 3425.1
(3)
P 		2030.7
(-1.3)
P 		1487
(-2.9)
P 		1465.8
(-3)
P 		1959
B 3453.6
(3)
P 		1998.3
(-1.6)
P 		1575.4
(-2.9)
P 		1554.7
(-3)
P 		1899
C 3277
(3)
P 		1811.8
(-1.5)
P 		1603.7
(-2.2)
P 		1333.1
(-3)
P 		1944
avr 3385.2
(3) 		1946.9
(-1.5) 		1555.4
(-2.7) 		1451.2
(-3) 		1934

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2151
1 2 3 4 5 6 range
A 2935
(2.4)
P 		3100.8
(3)
P 		1545.8
(-2.9)
P 		1508.6
(-3)
P 		1701.8
(-2.3)
P 		2038.2
(-1)
P 		1592
B 3310.2
(3)
P 		3054.2
(2.3)
P 		2189.6
(-0.1)
P 		2475
(0.7)
P 		1159
(-3)
P 		2062.9
(-0.5)
P 		2151
C 2650.9
(2.2)
P 		2856.5
(3)
P 		2408.9
(1.4)
P 		1949.8
(-0.3)
P 		1224.6
(-3)
P 		1860.1
(-0.7)
P 		1632
avr 2965.4
(2.9) 		3003.8
(3) 		2048.1
(-0.5) 		1977.8
(-0.7) 		1361.8
(-3) 		1987.1
(-0.7) 		1642
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1033
1 2 3 4 range
A 1247.1
(3)
P 		440.8
(-1.7)
P 		388.7
(-2)
P 		214.6
(-3)
P 		1033
B 1101.3
(3)
P 		493.5
(-1.7)
P 		365.1
(-2.7)
P 		331.3
(-3)
P 		770
C 1084.8
(3)
P 		520.9
(-0.9)
P 		558.9
(-0.7)
P 		224.8
(-3)
P 		860
avr 1144.4
(3) 		485.1
(-1.5) 		437.6
(-1.8) 		256.9
(-3) 		888

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 815
1 2 3 4 5 6 range
A 541
(-1.4)
P 		1102.8
(3)
P 		336.6
(-3)
P 		382.6
(-2.6)
P 		513.5
(-1.6)
P 		496.2
(-1.8)
P 		766
B 1151.8
(3)
P 		1016.6
(1.8)
P 		531.7
(-2.5)
P 		655.5
(-1.4)
P 		470.9
(-3)
P 		769.3
(-0.4)
P 		681
C 982.6
(3)
P 		871.6
(1.9)
P 		613.1
(-0.8)
P 		392.1
(-3)
P 		396.3
(-3)
P 		622.7
(-0.7)
P 		591
avr 891.8
(1.8) 		997
(3) 		493.8
(-2.6) 		476.7
(-2.8) 		460.2
(-3) 		629.4
(-1.1) 		537
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181623_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1934
Sequence Type Exemplar sequence
Representative Public ID ZK973.6 NCBI
Target Description ZK973.6 /REP_DB=WormBase Gene ID /WP=CE24752 /TR=Q9N4M4 /GB=AAF40010.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058952(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058952 NCBI Caenorhabditis elegans abnormal nuclear ANChorage family member (anc-1) (anc-1) mRNA, complete cds. 11/11 A 
GENEFINDER00000023526 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4321663:4358873:1 11/11 None
SNAP00000023506 ENSEMBL cdna:SNAP chromosome:CEL140:I:4334126:4358873:1 11/11 None
ZK973.6 ENSEMBL cdna:known chromosome:CEL140:I:4321096:4359081:1 gene:ZK973.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4355639-4358873(+) 100.0 100.0
chrI:4341051-4343028(+) 63.22 63.22
chrI:4346978-4348955(+) 63.22 63.22
chrI:4349865-4351842(+) 63.22 63.22
chrI:4352752-4354729(+) 63.22 63.22
chrI:4343938-4345591(+) 52.69 52.69
Public Domain and Genome References
Gene Title
Gene Symbol anc-1
Ensembl ZK973.6
Entrez Gene 172034 Entrez gene
SwissProt Q9N4M4 EMBL-EBI
WormBase CE33588 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1585356_AT similar to nesprin 1 isoform beta cfa
CANINE:1591393_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.11044.1.A1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.14004.1.A1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.14004.1.A1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFA.20729.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1680.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1700.1.S1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1717.1.S1_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1717.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1722.1.S1_S_AT similar to nesprin 1 isoform beta cfa
CANINE_2:CFAAFFX.1723.1.S1_AT similar to nesprin 1 isoform beta cfa
CHICKEN:GGA.15842.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGA.15842.1.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGA.18878.1.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGA.9526.1.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.24072.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8215.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8215.2.S1_S_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8600.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
CHICKEN:GGAAFFX.8667.1.S1_AT spectrin repeat containing, nuclear envelope 1 gga
HG-U133_PLUS_2:215350_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A_2:215350_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A:215350_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:209447_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A:209447_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133A_2:209447_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBA:RC_AA452410_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBB:RC_AA227537_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBB:RC_AA218915_AT spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBC:RC_N23629_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:G2895592_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.272567.0.S1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.241420.0.S1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.192102.0.A1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:HS.161707.0.A1_3P_AT spectrin repeat containing, nuclear envelope 1 hs
U133_X3P:215350_3P_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:232027_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133B:232027_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:244070_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133B:244070_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133_PLUS_2:244144_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U133B:244144_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95AV2:38113_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95B:42634_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95C:63705_F_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95C:63906_F_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:72970_G_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:85140_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:88102_AT spectrin repeat containing, nuclear envelope 1 hs
HG-U95C:63924_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95E:67418_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:68235_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:72968_AT Spectrin repeat containing, nuclear envelope 1 hs
HG-U95D:82696_AT Spectrin repeat containing, nuclear envelope 1 hs
HU35KSUBC:RC_N71774_AT Spectrin repeat containing, nuclear envelope 1 hs
RG-U34A:X95466_AT CPG2 protein rn
RG-U34C:RC_AI070400_AT CPG2 protein rn
RG-U34C:RC_AI072953_S_AT CPG2 protein rn
RAE230A:1370264_AT CPG2 protein rn
RAT230_2:1370264_AT CPG2 protein rn
RAE230A:1398263_AT CPG2 protein rn
RAT230_2:1398263_AT CPG2 protein rn
RAE230B:1378886_X_AT CPG2 protein rn
RAT230_2:1378886_X_AT CPG2 protein rn
RAE230B:1398499_AT CPG2 protein rn
RAT230_2:1398499_AT CPG2 protein rn
RAE230B:1396587_AT CPG2 protein rn
RAT230_2:1396587_AT CPG2 protein rn
RAE230B:1398155_AT CPG2 protein rn
RAT230_2:1398155_AT CPG2 protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6915 apoptosis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3779 actin binding inferred from electronic annotation QuickGO AmiGO
5515 protein binding inferred from electronic annotation QuickGO AmiGO
3779 actin binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF40010 Abnormal nuclear anchorage protein 1 [Caenorhabditis elegans] ref|NP_491353.2| abnormal nuclear ANChorage family member (anc-1) [Caenorhabditis elegans] sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog) 0.0
blast tpg|DAA04553.1 TPA: ANC-1 [Caenorhabditis elegans] 0.0
blast AAN35200 ANC-1 [Caenorhabditis elegans] 0.0
blast CAE66823 Hypothetical protein CBG12190 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 4.3E-30
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.6E-28
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 3.3E-17
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.6E-28
Sequence
Target Sequence 
>C. ELEGANS:181623_AT
cgccacaatgctcaacgatgctattgctcatctcgaaaaccagaaggttgttgttgctag
aagcgagaaggaccgcaaatttgtcgaaagcagcacttcgatagatcttgaccaactttt
agctgaagctaaacgccttcttaaagaaatcgaaccaagacttcaactcgcacagcctga
ccatgacaatgaagatgatgaagatgaagagaaaggaagcgatgagaagccgtatgacgt
tagagcagcggcagaagtgctctcagcactttatccagatgaacatccgcacaatgttct
tcgaaacattggttttgaagaactcccgtcggattctgaatcaagaagtgaattcgactc
acttgacagtcgatctgatggattactttccccaattcctgacgattcgactctcagtga
agaacaactccgacgacaaagatcccgttggagacgggtactcagaactgctttgccact
tc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCCACAATGCTCAACGATGCTATT 7 259 2232 Antisense
TGCTATTGCTCATCTCGAAAACCAG 560 579 2250 Antisense
GCAGCACTTCGATAGATCTTGACCA 498 313 2321 Antisense
CGAACCAAGACTTCAACTCGCACAG 426 251 2382 Antisense
CTCGCACAGCCTGACCATGACAATG 432 233 2398 Antisense
TGCTCTCAGCACTTTATCCAGATGA 543 581 2489 Antisense
GAAGAACTCCCGTCGGATTCTGAAT 56 341 2548 Antisense
CTGATGGATTACTTTCCCCAATTCC 176 239 2606 Antisense
CCAATTCCTGACGATTCGACTCTCA 305 277 2623 Antisense
AACTCCGACGACAAAGATCCCGTTG 417 141 2657 Antisense
GTACTCAGAACTGCTTTGCCACTTC 429 459 2689 Antisense
Sequence Source Affymetrix Proprietary Database