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[181617_at]

Description T08B1.5 /REP_DB=WormBase Gene ID /WP=CE13419 /TR=O44559 /GB=AAB94179.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 64      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 30.8
(-2)
A 		12.3
(-3)
A 		35.4
(-1.7)
A 		118.2
(3)
A 		106
B 8.7
(-3)
A 		53.1
(3)
A 		14.4
(-2.2)
A 		41.2
(1.4)
A 		44
C 30.5
(-3)
A 		64.1
(-0.4)
A 		35.6
(-2.6)
A 		108.8
(3)
A 		78
avr 23.3
(-3) 		43.2
(-1.2) 		28.5
(-2.5) 		89.4
(3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 32.5
(0.2)
A 		13.8
(-2)
A 		56
(3)
M 		33
(0.3)
A 		42.6
(1.4)
P 		5.4
(-3)
A 		51
B 17.3
(-3)
A 		46.6
(-0.4)
A 		84.9
(3)
M 		28.8
(-2)
A 		50.6
(-0)
A 		55.2
(0.4)
A 		68
C 24.3
(-1.7)
A 		39.5
(-0.5)
A 		8.4
(-3)
A 		37.3
(-0.7)
A 		47.7
(0.1)
A 		83.8
(3)
A 		75
avr 24.7
(-3) 		33.3
(-0.9) 		49.8
(3) 		33
(-1) 		47
(2.3) 		48.1
(2.6) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 11.2
(0.5)
A 		2.7
(-3)
A 		9.3
(-0.3)
A 		17.3
(3)
A 		15
B 2.8
(-3)
A 		13.1
(3)
A 		3.3
(-2.7)
A 		8.8
(0.5)
A 		10
C 10.1
(-3)
A 		18.4
(3)
A 		12.4
(-1.3)
A 		18.3
(2.9)
A 		8
avr 8
(-3) 		11.4
(0) 		8.3
(-2.7) 		14.8
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 6
(-0.6)
A 		4.9
(-1.1)
A 		12.2
(2.6)
M 		8.4
(0.7)
A 		12.9
(3)
P 		1.3
(-3)
A 		12
B 6
(-3)
A 		15.5
(0.9)
A 		20.6
(3)
M 		7.6
(-2.3)
A 		20.5
(3)
A 		20.6
(3)
A 		15
C 9
(-1.4)
A 		12.1
(-0.7)
A 		2.1
(-3)
A 		7.5
(-1.8)
A 		15.4
(0.1)
A 		28.1
(3)
A 		26
avr 7
(-3) 		10.8
(-0.6) 		11.6
(-0.2) 		7.8
(-2.5) 		16.3
(2.8) 		16.7
(3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181617_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15402
Sequence Type Exemplar sequence
Representative Public ID T08B1.5 NCBI
Target Description T08B1.5 /REP_DB=WormBase Gene ID /WP=CE13419 /TR=O44559 /GB=AAB94179.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071140
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
T08B1.5 ENSEMBL cdna:known chromosome:CEL140:V:1975246:1977064:1 gene:T08B1.5 None
SNAP00000025789 ENSEMBL cdna:SNAP chromosome:CEL140:V:1976797:1977093:1 None
NM_071140 NCBI Caenorhabditis elegans T08B1.5 (T08B1.5) mRNA, complete cds. None
GENEFINDER00000025796 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1969384:1977093:1 None
SNAP00000025788 ENSEMBL cdna:SNAP chromosome:CEL140:V:1969581:1976647:1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1975219-1977093(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T08B1.5
Entrez Gene 188270 Entrez gene
SwissProt O44559 EMBL-EBI
WormBase CE35762 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94180 Hypothetical protein T08B1.6 [Caenorhabditis elegans] ref|NP_503540.1| T08B1.6 [Caenorhabditis elegans] 0.0
blast CAE66776 Hypothetical protein CBG12133 [Caenorhabditis briggsae] 0.0
blast AAH43756 Facl2-prov protein [Xenopus laevis] 1.0E-108
blast CAG06540 unnamed protein product [Tetraodon nigroviridis] 2.0E-98
blast AAB94179 Hypothetical protein T08B1.5 [Caenorhabditis elegans] ref|NP_503541.2| T08B1.5 [Caenorhabditis elegans] 3.0E-82
blast CAA83594 Hypothetical protein C05B5.6 [Caenorhabditis elegans] ref|NP_499220.2| C05B5.6 [Caenorhabditis elegans] sp|P34294|YKO6_CAEEL Hypothetical protein C05B5.6 in chromosome III 2.0E-20
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 3.0E-85
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 4.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 1.2E-75
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 4.2E-24
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 1.9E-46
Sequence
Target Sequence 
>C. ELEGANS:181617_AT
acaatcatttcctctactggttcgatgggcttgccgatgggacactgacattggactcga
ttgaccggtttgtgattttcttcaggaaaaatgctattgagtcactcacattaccgcccc
cgttatattttccagttttttgtgaacacaatgctctttacaggttctcgccacaagcag
ttccccgcagcttccaggccacaattgacaaaattacaaatatcaaggatgtttatgtgt
attttgacaagtcaatttggagcattgtctgtttggaacagtggaaaaaagcgaaaactc
tctccttggggttttctaccgttgccaagttttctaagatcgtcagacacgttgctcact
ttacctcaatgagtttcaactgttcgaaatcatttttatccgaagccgatattctgagca
tcagagatatcgttctcgtgaaatccaagaatatggataactgctctttcgaagatgtga
aatatgataaaaaccaatttatgaatgccttcaatgtttcccaatctgcacaatcatttg
gatcgttttcctatcaaatcggca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAATCATTTCCTCTACTGGTTCGA 264 115 233 Antisense
GGTTCGATGGGCTTGCCGATGGGAC 99 507 251 Antisense
GGACTCGATTGACCGGTTTGTGATT 596 523 285 Antisense
TATTGAGTCACTCACATTACCGCCC 401 665 327 Antisense
CACAATGCTCTTTACAGGTTCTCGC 248 195 379 Antisense
AGCTTCCAGGCCACAATTGACAAAA 78 79 421 Antisense
TTCTACCGTTGCCAAGTTTTCTAAG 484 687 546 Antisense
AAGATCGTCAGACACGTTGCTCACT 619 155 568 Antisense
ATCCGAAGCCGATATTCTGAGCATC 276 37 630 Antisense
TGAATGCCTTCAATGTTTCCCAATC 643 575 734 Antisense
GGATCGTTTTCCTATCAAATCGGCA 111 513 772 Antisense
Sequence Source Affymetrix Proprietary Database