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[181614_at]

Description F31D5.1 /REP_DB=WormBase Gene ID /WP=CE19825 /TR=Q19933 /GB=AAC71106.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 19      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 43
(-2.3)
A 		34.3
(-3)
A 		34.7
(-3)
A 		107.5
(3)
A 		73
B 44.8
(0.3)
P 		44
(0.2)
A 		65.4
(3)
A 		19.8
(-3)
A 		46
C 36.7
(0.8)
A 		53.3
(3)
A 		8.1
(-3)
A 		43.7
(1.7)
P 		45
avr 41.5
(-1.4) 		43.9
(-0.8) 		36.1
(-3) 		57
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 30.3
(-3)
A 		39.6
(-1.6)
A 		33.8
(-2.5)
A 		31.3
(-2.8)
A 		69.7
(3)
P 		61
(1.7)
P 		39
B 45.8
(-0.9)
A 		25.8
(-2.5)
M 		65.2
(0.7)
P 		19.8
(-3)
A 		93.2
(3)
P 		80.4
(2)
P 		73
C 47.5
(1.6)
A 		9.2
(-3)
A 		29
(-0.6)
A 		53.9
(2.3)
P 		44.9
(1.3)
A 		59.6
(3)
P 		50
avr 41.2
(-0.8) 		24.9
(-3) 		42.7
(-0.6) 		35
(-1.6) 		69.3
(3) 		67
(2.7) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 15.7
(3)
A 		7.5
(-3)
A 		9.1
(-1.8)
A 		15.7
(3)
A 		8
B 14.3
(2.5)
P 		10.9
(0.7)
A 		15.2
(3)
A 		4.2
(-3)
A 		11
C 12.1
(1.5)
A 		15.3
(3)
A 		2.8
(-3)
A 		7.4
(-0.8)
P 		13
avr 14
(3) 		11.2
(-0.4) 		9
(-3) 		9.1
(-2.9) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 5.6
(-3)
A 		14.1
(0.3)
A 		7.4
(-2.3)
A 		7.9
(-2.1)
A 		21
(3)
P 		14.9
(0.6)
P 		15
B 15.9
(-1)
A 		8.6
(-2.4)
M 		15.8
(-1.1)
P 		5.2
(-3)
A 		37.9
(3)
P 		30
(1.6)
P 		33
C 17.6
(2.2)
A 		2.8
(-3)
A 		7.4
(-1.4)
A 		10.8
(-0.2)
P 		14.5
(1.1)
A 		19.9
(3)
P 		17
avr 13
(-1.2) 		8.5
(-2.8) 		10.2
(-2.2) 		8
(-3) 		24.5
(3) 		21.6
(1.9) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181614_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5710
Sequence Type Exemplar sequence
Representative Public ID F31D5.1 NCBI
Target Description F31D5.1 /REP_DB=WormBase Gene ID /WP=CE19825 /TR=Q19933 /GB=AAC71106.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062341(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062341 NCBI Caenorhabditis elegans F31D5.1 (F31D5.1) mRNA, complete cds. 11/11 None
SNAP00000042935 ENSEMBL cdna:SNAP chromosome:CEL140:II:4219373:4221763:1 9/11 None
GENEFINDER00000042950 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4219373:4226247:1 11/11 None
F31D5.1 ENSEMBL cdna:known chromosome:CEL140:II:4219373:4222297:1 gene:F31D5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4219371-4222296(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F31D5.1
Entrez Gene 185151 Entrez gene
SwissProt Q19933 EMBL-EBI
WormBase CE19825 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC71106 Hypothetical protein F31D5.1 [Caenorhabditis elegans] ref|NP_494742.1| F31D5.1 [Caenorhabditis elegans] 0.0
blast AAK31411 Hypothetical protein C16A11.5 [Caenorhabditis elegans] ref|NP_494743.2| C16A11.5 [Caenorhabditis elegans] 0.0
blast AAK84613 Hypothetical protein Y52E8A.4 [Caenorhabditis elegans] ref|NP_494758.1| Y52E8A.4 [Caenorhabditis elegans] 1.0E-154
blast AAK84613 Hypothetical protein Y52E8A.4 [Caenorhabditis elegans] ref|NP_494758.1| Y52E8A.4 [Caenorhabditis elegans] 1.0E-139
blast CAE74272 Hypothetical protein CBG21963 [Caenorhabditis briggsae] 1.0E-105
blast AAC71106 Hypothetical protein F31D5.1 [Caenorhabditis elegans] ref|NP_494742.1| F31D5.1 [Caenorhabditis elegans] 6.0E-66
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 1.60000002384186
scop a.4.5.DNA-binding protein Mj223 All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; DNA-binding protein Mj223 9.0
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 3.0E-52
Pfam IPR010291 EMBL-EBI Eukaryotic protein of unknown function DUF895 1.1E-89
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494742.1 10 9-31,51-73,80-97,102-121,141-163,178-196,227-249,264-286,298-317,332-354
F31D5.1 10 9-31,51-73,80-97,102-121,141-163,178-196,227-249,264-286,298-317,332-354
SNAP00000042935 6 9-31,51-73,102-121,136-158,170-189,204-226
GENEFINDER00000042950 11 9-31,51-73,80-97,102-121,141-163,178-196,227-249,264-286,298-317,332-354,374-396
Sequence
Target Sequence 
>C. ELEGANS:181614_AT
ttcgatctacccattctgcattaaattcacatcgaaccttgcctccaataccaccagaat
cctaacaatcggaatgattgtcaccggctgcggtcaggttatcggatcgatttttgtggc
aataattggaaataaaattcgaaaatttgggcaacatgttctaattcttggcgcccttat
tttgcatgttatcctatttctgatgatctctttatcattcccaaatgatgctccacttca
tcatactgatggaaatggcccagtgtttccgttgagtgtctacttggctatggcaatttc
tgcacttttgggtttcggtgacgcaattttgcagacccagatttactcgtatatcgccaa
atattatcagagagacagtagtagtgtgttttcgattttcagattttcaagtaagcccct
gtcgtggttctttcgatttcaaacgatgttggccgcaaatcacaacaatcggtctcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGATCTACCCATTCTGCATTAAA 700 695 744 Antisense
TAAATTCACATCGAACCTTGCCTCC 157 641 765 Antisense
TGTCACCGGCTGCGGTCAGGTTATC 508 557 822 Antisense
CAACATGTTCTAATTCTTGGCGCCC 427 189 895 Antisense
GGCGCCCTTATTTTGCATGTTATCC 301 543 913 Antisense
GATGCTCCACTTCATCATACTGATG 591 413 970 Antisense
GTTTCCGTTGAGTGTCTACTTGGCT 101 447 1008 Antisense
GCACTTTTGGGTTTCGGTGACGCAA 634 313 1045 Antisense
GACCCAGATTTACTCGTATATCGCC 469 381 1077 Antisense
AGATTTTCAAGTAAGCCCCTGTCGT 269 63 1144 Antisense
GCAAATCACAACAATCGGTCTCGCC 485 317 1198 Antisense
Sequence Source Affymetrix Proprietary Database