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[181607_at]

Description B0273.1 /REP_DB=WormBase Gene ID /WP=CE16787 /TR=O44170 /GB=AAB88354.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 21      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 83.1
(0.2)
A 		17.4
(-2.9)
A 		15.6
(-3)
A 		143
(3)
A 		127
B 113.7
(3)
A 		61.9
(-0.1)
A 		13.6
(-3)
A 		59.5
(-0.2)
A 		100
C 82.6
(-0.6)
A 		77.5
(-0.9)
A 		40.6
(-3)
A 		145
(3)
A 		104
avr 93.1
(1.5) 		52.3
(-1.1) 		23.3
(-3) 		115.8
(3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 171.5
(2.2)
A 		152.5
(0.4)
A 		144.5
(-0.4)
A 		116.9
(-3)
A 		155.4
(0.7)
A 		179.8
(3)
A 		63
B 166.6
(1.1)
A 		149.7
(-0.3)
A 		149.8
(-0.3)
A 		118.2
(-3)
A 		188.5
(3)
P 		171.8
(1.6)
A 		70
C 163.7
(-0)
A 		144.4
(-1.3)
A 		118.3
(-3)
A 		189.1
(1.6)
A 		210.6
(3)
A 		156.1
(-0.5)
M 		92
avr 167.3
(0.8) 		148.9
(-1.6) 		137.5
(-3) 		141.4
(-2.5) 		184.8
(3) 		169.2
(1) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 30.3
(3)
A 		3.8
(-3)
A 		4.1
(-2.9)
A 		20.9
(0.9)
A 		27
B 36.2
(3)
A 		15.3
(-0.8)
A 		3.2
(-3)
A 		12.7
(-1.3)
A 		33
C 27.3
(3)
A 		22.3
(0.7)
A 		14.2
(-3)
A 		24.5
(1.7)
A 		13
avr 31.3
(3) 		13.8
(-1.4) 		7.2
(-3) 		19.4
(0) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 31.6
(-2.5)
A 		54.2
(3)
A 		31.5
(-2.6)
A 		29.7
(-3)
A 		46.9
(1.2)
A 		43.8
(0.5)
A 		25
B 58
(0.5)
A 		49.8
(-0.5)
A 		36.4
(-2.3)
A 		31.3
(-3)
A 		76.6
(3)
P 		64.1
(1.3)
A 		45
C 60.7
(1.8)
A 		44.1
(-0.8)
A 		30.1
(-3)
A 		38
(-1.8)
A 		68.2
(3)
A 		52.2
(0.5)
M 		38
avr 50.1
(0.3) 		49.4
(0.2) 		32.7
(-3) 		33
(-2.9) 		63.9
(3) 		53.4
(1) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181607_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10672
Sequence Type Exemplar sequence
Representative Public ID B0273.1 NCBI
Target Description B0273.1 /REP_DB=WormBase Gene ID /WP=CE16787 /TR=O44170 /GB=AAB88354.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068420(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068420 NCBI Caenorhabditis elegans B0273.1 (B0273.1) mRNA, complete cds. 11/11 None
SNAP00000012463 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5494221:5495191:1 10/11 None
B0273.1 ENSEMBL cdna:known chromosome:CEL140:IV:5493991:5495192:1 gene:B0273.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000012470 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5494226-5495197(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0273.1
Entrez Gene 177333 Entrez gene
SwissProt O44170 EMBL-EBI
WormBase CE16787 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB88354 Hypothetical protein B0273.1 [Caenorhabditis elegans] ref|NP_500821.1| B0273.1 [Caenorhabditis elegans] 1.0E-124
blast AAB88354 Hypothetical protein B0273.1 [Caenorhabditis elegans] ref|NP_500821.1| B0273.1 [Caenorhabditis elegans] 1.0E-118
blast CAE59484 Hypothetical protein CBG02869 [Caenorhabditis briggsae] 3.0E-68
blast CAE59484 Hypothetical protein CBG02869 [Caenorhabditis briggsae] 1.0E-62
blast CAA91379 Hypothetical protein B0334.10 [Caenorhabditis elegans] ref|NP_496461.1| B0334.10 [Caenorhabditis elegans] 7.0E-58
blast CAA91379 Hypothetical protein B0334.10 [Caenorhabditis elegans] ref|NP_496461.1| B0334.10 [Caenorhabditis elegans] 1.0E-57
Sequence
Target Sequence 
>C. ELEGANS:181607_AT
accatccaaagctgctctgctgaaaaacaagccggtcgatatctacgctagggccattta
cccgtacaatcgaaaaaacattcgtcactggattgacggacacgtgtcgtataagccaca
tcatctcgtccagacactcaattttctcgccgacgatgccgagtattcggcgaaaacgat
tgtcatcgacctgcccgggagatctacaatgattgagtcgacgggatatgcaatcaaaat
gaacaacagtaaaccacttcttcgtgcttttctggaaaatgacacggcttgcgctgctca
attcgaatcgaaatccgtgctgaaatgcctcccaagagttgcaaaaccggattctctgaa
atccagcaaaaaagctccaatcgaagcaaagaatgaagatccctgcttcatgattccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCATCCAAAGCTGCTCTGCTGAAA 612 85 192 Antisense
GACACGTGTCGTATAAGCCACATCA 654 367 290 Antisense
TCGTCCAGACACTCAATTTTCTCGC 577 605 317 Antisense
ATTTTCTCGCCGACGATGCCGAGTA 184 15 332 Antisense
GAAAACGATTGTCATCGACCTGCCC 165 355 363 Antisense
CAGTAAACCACTTCTTCGTGCTTTT 433 205 438 Antisense
TGGAAAATGACACGGCTTGCGCTGC 406 547 464 Antisense
CTTGCGCTGCTCAATTCGAATCGAA 380 219 479 Antisense
GCTGAAATGCCTCCCAAGAGTTGCA 113 295 510 Antisense
GTTGCAAAACCGGATTCTCTGAAAT 312 439 529 Antisense
GAAGATCCCTGCTTCATGATTCCAT 131 339 586 Antisense
Sequence Source Affymetrix Proprietary Database