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[181603_at]

Description T05B4.4 /REP_DB=WormBase Gene ID /WP=CE13199 /TR=O16423 /GB=AAC69056.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 08      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 2
(-3)
A 		8.9
(-1.9)
A 		26
(0.7)
A 		40.7
(3)
A 		39
B 4.1
(-3)
A 		4.3
(-3)
A 		6.6
(-2.6)
A 		43.1
(3)
A 		39
C 3.9
(-3)
A 		4.6
(-1.4)
A 		6.6
(3)
A 		5.1
(-0.3)
A 		3
avr 3.3
(-3) 		5.9
(-2.4) 		13.1
(-0.8) 		29.6
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 7.8
(2)
A 		3.8
(-3)
A 		6
(-0.3)
A 		8.6
(3)
A 		7.3
(1.4)
A 		6.4
(0.3)
A 		5
B 41.5
(1.4)
A 		30.6
(0.1)
A 		7.5
(-2.5)
A 		3.5
(-3)
A 		55.5
(3)
A 		6.8
(-2.6)
A 		52
C 24.7
(1.3)
A 		8.8
(-1.8)
A 		29.1
(2.2)
A 		8.5
(-1.9)
A 		33.4
(3)
A 		2.7
(-3)
A 		31
avr 24.7
(1.3) 		14.4
(-1) 		14.2
(-1) 		6.9
(-2.6) 		32.1
(3) 		5.3
(-3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 0.7
(-3)
A 		1.9
(-1.8)
A 		6.8
(3)
A 		6
(2.2)
A 		6
B 1.3
(-2.9)
A 		1.1
(-3)
A 		1.5
(-2.7)
A 		9.2
(3)
A 		8
C 1.3
(-1.3)
A 		1.3
(-1.3)
A 		2.3
(3)
A 		0.9
(-3)
A 		1
avr 1.1
(-3) 		1.4
(-2.6) 		3.5
(0.3) 		5.4
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 1.4
(-2.3)
A 		1.4
(-2.3)
A 		1.3
(-3)
A 		2.2
(3)
A 		2.2
(3)
A 		1.6
(-1)
A 		1
B 14.5
(0.8)
A 		10.2
(-0.4)
A 		1.8
(-2.8)
A 		0.9
(-3)
A 		22.6
(3)
A 		2.5
(-2.6)
A 		22
C 9.1
(2)
A 		2.7
(-1.9)
A 		7.4
(0.9)
A 		1.7
(-2.5)
A 		10.8
(3)
A 		0.9
(-3)
A 		10
avr 8.3
(0.9) 		4.8
(-1.1) 		3.5
(-1.9) 		1.6
(-3) 		11.9
(3) 		1.7
(-2.9) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181603_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15635
Sequence Type Exemplar sequence
Representative Public ID T05B4.4 NCBI
Target Description T05B4.4 /REP_DB=WormBase Gene ID /WP=CE13199 /TR=O16423 /GB=AAC69056.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071750(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071750 NCBI Caenorhabditis elegans T05B4.4 (T05B4.4) mRNA, complete cds. 11/11 None
SNAP00000031067 ENSEMBL cdna:SNAP chromosome:CEL140:V:4127681:4129415:1 11/11 None
GENEFINDER00000031076 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4127681:4129415:1 11/11 None
T05B4.4 ENSEMBL cdna:known chromosome:CEL140:V:4127363:4129413:1 gene:T05B4.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000031066 1/11 Cross Hyb Matching Probes None
GENEFINDER00000031080 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4127362-4129415(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T05B4.4
Entrez Gene 188100 Entrez gene
SwissProt O16423 EMBL-EBI
WormBase CE30826 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE65452 Hypothetical protein CBG10417 [Caenorhabditis briggsae] 1.0E-120
blast CAE65453 Hypothetical protein CBG10418 [Caenorhabditis briggsae] 1.0E-116
blast AAC69054 Hypothetical protein T05B4.3 [Caenorhabditis elegans] ref|NP_504152.1| T05B4.3 [Caenorhabditis elegans] 3.0E-92
blast AAC69056 Hypothetical protein T05B4.4 [Caenorhabditis elegans] ref|NP_504151.2| T05B4.4 [Caenorhabditis elegans] 8.0E-86
blast CAE65452 Hypothetical protein CBG10417 [Caenorhabditis briggsae] 2.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.7E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.6E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.9E-14
Sequence
Target Sequence 
>C. ELEGANS:181603_AT
tcgcatgctctaacacaatctctgatgcagcttgccaagctctctacgctgctccagaca
ccactgccggatacccatcagctggaaccgatgctgaacgaccattgaactgctacacaa
ccgccacagctactccagcagctattgttcaagacatgaagactgctgctttggagactt
gtgccaagacttgtggattgtgctgccaaactgatgcatacagttgcaacccacgcctcg
actgctcaacaatcaccactgctcaatgtaactctatcatctggagatccatcattgcca
ccgactgcccatctgcttgcggattctgtaacgagggaggatgtgttgatgctcttgttg
actgtgccactgatatctcaatctgcactacagttggaatgcaagatttcgtgaatacct
actgccaaagaacatgtggaagatgcccatcatcaagtacaacctcttcaagctccacag
cttcatcttcctccacatgcacttcttacaatgctgacagttcatctctctgttccgatt
gggcggccaatggattctgcaccaatgcgttctacaccagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGCATGCTCTAACACAATCTCTGA 124 599 269 Antisense
AATCTCTGATGCAGCTTGCCAAGCT 7 169 285 Antisense
CCGGATACCCATCAGCTGGAACCGA 106 265 335 Antisense
GGAACCGATGCTGAACGACCATTGA 14 531 352 Antisense
GACCATTGAACTGCTACACAACCGC 604 383 368 Antisense
CCACAGCTACTCCAGCAGCTATTGT 356 273 392 Antisense
GTGGATTGTGCTGCCAAACTGATGC 260 489 461 Antisense
TCAAGTACAACCTCTTCAAGCTCCA 107 633 721 Antisense
CACATGCACTTCTTACAATGCTGAC 13 197 762 Antisense
CAATGCTGACAGTTCATCTCTCTGT 115 183 777 Antisense
TGCACCAATGCGTTCTACACCAGTG 587 577 826 Antisense
Sequence Source Affymetrix Proprietary Database