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[181592_s_at]

Description C26E6.5 /REP_DB=WormBase Gene ID /WP=CE01163 /TR=Q18223 /GB=AAA21159.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 95      6grp_total > 88      4grp_total > 13      6grp_highest_sub > Da      6grp_cgc > 69      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 387
1 2 3 4 range
A 478.5
(-0.2)
P 		352.3
(-2.2)
P 		300.8
(-3)
P 		687.8
(3)
P 		387
B 503.4
(0.6)
P 		333.3
(-2.6)
P 		312.6
(-3)
P 		634.4
(3)
P 		322
C 477.2
(0.7)
P 		345.6
(-3)
P 		363
(-2.5)
P 		561.5
(3)
P 		216
avr 486.4
(0.2) 		343.7
(-2.6) 		325.5
(-3) 		627.9
(3) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 852
1 2 3 4 5 6 range
A 523.5
(-1.6)
P 		514.7
(-1.7)
P 		329.6
(-3)
P 		538.7
(-1.5)
P 		861
(0.8)
P 		1160.4
(3)
P 		831
B 553
(-1.4)
P 		443.1
(-2.2)
P 		349.5
(-3)
P 		563.8
(-1.3)
P 		909
(1.5)
P 		1093.4
(3)
P 		744
C 546.4
(-2.1)
P 		440.8
(-3)
P 		492.9
(-2.6)
P 		556.9
(-2.1)
P 		823.4
(0.1)
P 		1181.1
(3)
P 		740
avr 541
(-1.8) 		466.2
(-2.4) 		390.7
(-3) 		553.1
(-1.7) 		864.5
(0.8) 		1145
(3) 		754
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 174.2
(3)
P 		76.5
(-3)
P 		78.6
(-2.9)
P 		100.7
(-1.5)
P 		98
B 160.5
(3)
P 		82.3
(-2.3)
P 		72.4
(-3)
P 		135.2
(1.3)
P 		88
C 158
(3)
P 		99.4
(-2.6)
P 		126.5
(0)
P 		94.7
(-3)
P 		63
avr 164.2
(3) 		86.1
(-3) 		92.5
(-2.5) 		110.2
(-1.1) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 336
1 2 3 4 5 6 range
A 96.5
(-2.3)
P 		183.1
(0.2)
P 		71.8
(-3)
P 		136.6
(-1.2)
P 		259.8
(2.4)
P 		282.5
(3)
P 		211
B 192.4
(-1)
P 		147.5
(-1.8)
P 		84.9
(-3)
P 		149.3
(-1.8)
P 		369.3
(2.3)
P 		407.7
(3)
P 		323
C 202.5
(-1.1)
P 		134.5
(-2.5)
P 		125.5
(-2.7)
P 		112
(-3)
P 		266.4
(0.3)
P 		395.4
(3)
P 		283
avr 163.8
(-1.4) 		155
(-1.6) 		94.1
(-3) 		132.6
(-2.1) 		298.5
(1.6) 		361.9
(3) 		268
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181592_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5991
Sequence Type Exemplar sequence
Representative Public ID C26E6.5 NCBI
Target Description C26E6.5 /REP_DB=WormBase Gene ID /WP=CE01163 /TR=Q18223 /GB=AAA21159.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065645(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065645 NCBI Caenorhabditis elegans F-box SyNaptic Protein family member (fsn-1) (fsn-1) mRNA, complete cds. 11/11 None
SNAP00000014702 ENSEMBL cdna:SNAP chromosome:CEL140:III:4937322:4939098:1 11/11 None
GENEFINDER00000014708 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4937322:4944024:1 10/11 None
C26E6.5 ENSEMBL cdna:known chromosome:CEL140:III:4937299:4939391:1 gene:C26E6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4937321-4939098(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol fsn-1
Ensembl C26E6.5
Entrez Gene 175667 Entrez gene
SwissProt Q18223 EMBL-EBI
WormBase CE01163 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1636980_S_AT dm
DROSGENOME1:142935_AT dm
HU35KSUBA:RC_AA425304_AT F-box protein 45 hs
HU35KSUBB:RC_W44928_AT F-box protein 45 hs
U133_X3P:HS.6994.0.S2_3P_AT F-box protein 45 hs
U133_X3P:HS.6994.0.S1_3P_AT F-box protein 45 hs
HG-U133_PLUS_2:225099_AT F-box protein 45 hs
HG-U133B:225099_AT F-box protein 45 hs
HG-U133_PLUS_2:225100_AT F-box protein 45 hs
HG-U133B:225100_AT F-box protein 45 hs
HG-U95C:62548_AT F-box protein 45 hs
HG-U95C:64688_AT F-box protein 45 hs
HG-U95E:86043_AT F-box protein 45 hs
MG-U74AV2:93217_AT F-box protein 45 mm
MU11KSUBA:C78482_RC_AT F-box protein 45 mm
MG-U74BV2:115502_AT F-box protein 45 mm
MOE430A:1428742_AT F-box protein 45 mm
MOUSE430A_2:1428742_AT F-box protein 45 mm
MOUSE430_2:1428742_AT F-box protein 45 mm
MOE430A:1452895_AT F-box protein 45 mm
MOUSE430A_2:1452895_AT F-box protein 45 mm
MOUSE430_2:1452895_AT F-box protein 45 mm
MG-U74CV2:140839_AT F-box protein 45, mRNA (cDNA clone MGC:19444 IMAGE:3499785) mm
MOE430B:1440824_AT F-box protein 45, mRNA (cDNA clone MGC:19444 IMAGE:3499785) mm
MOUSE430_2:1440824_AT F-box protein 45, mRNA (cDNA clone MGC:19444 IMAGE:3499785) mm
MOE430B:1458747_AT F-box protein 45, mRNA (cDNA clone MGC:19444 IMAGE:3499785) mm
MOUSE430_2:1458747_AT F-box protein 45, mRNA (cDNA clone MGC:19444 IMAGE:3499785) mm
MU19KSUBB:TC29315_AT F-box protein 45, mRNA (cDNA clone MGC:19444 IMAGE:3499785) mm
Protein Similarities
Method ID Description E-Value
blast AAA21159 F-box synaptic protein protein 1 [Caenorhabditis elegans] ref|NP_498046.1| F-box SyNaptic Protein family member (fsn-1) [Caenorhabditis elegans] sp|Q18223|FBSP1_CAEEL F-box/SPRY-domain protein 1 (F-box synaptic protein 1) 0.0
blast AAA21158 Hypothetical protein C26E6.4 [Caenorhabditis elegans] ref|NP_498047.1| RNA Polymerase II (B) subunit family member (rpb-2) [Caenorhabditis elegans] sp|Q10578|RPB2_CAEEL DNA-directed RNA polymerase II second largest subunit (RNA polymerase I subunit 2) 0.0
blast CAE71308 Hypothetical protein CBG18197 [Caenorhabditis briggsae] 0.0
blast NP_000929 DNA directed RNA polymerase II polypeptide B [Homo sapiens] gb|AAH23503.2| DNA directed RNA polymerase II polypeptide B [Homo sapiens] emb|CAA45124.1| RNA polymerase II 140 kDa subunit [Homo sapiens] gb|AAB23139.1| RNA polymerase II second largest subunit, RNA polymerase B second largest subunit [human, HeLa cells, Peptide, 1174 aa] ref|XP_214021.3| PREDICTED: similar to DNA-directed RNA polymerase II 140 kDa polypeptide (RNA polymerase II subunit 2) (RPB2) [Rattus norvegicus] sp|P30876|RPB2_HUMAN DNA-directed RNA polymerase II 140 kDa polypeptide (RNA polymerase II subunit 2) (RPB2) 0.0
blast CAE71307 Hypothetical protein CBG18196 [Caenorhabditis briggsae] 1.0E-160
blast XP_397079 PREDICTED: similar to CG4643-PA, isoform A [Apis mellifera] 2.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 1.3E-10
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 7.6E-18
Pfam IPR007641 EMBL-EBI RNA polymerase Rpb2, domain 7 1.6E-51
Pfam IPR007644 EMBL-EBI RNA polymerase beta subunit 1.5E-112
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 2.5E-15
Pfam IPR007642 EMBL-EBI RNA polymerase Rpb2, domain 2 3.8E-102
Pfam IPR007645 EMBL-EBI RNA polymerase Rpb2, domain 3 3.8E-31
Pfam IPR007646 EMBL-EBI RNA polymerase Rpb2, domain 4 6.4E-39
Pfam IPR007647 EMBL-EBI RNA polymerase Rpb2, domain 5 2.2E-28
Pfam IPR007120 EMBL-EBI RNA polymerase Rpb2, domain 6 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:181592_S_AT
tccagaagcagcagtttccgatcccttcctgctcgccgagctgggatctgcaaaaaagaa
gctgagagcctggtattttgcctggaatacgagcgatatttcaagaaataactacatccg
taccaacggtttcactgttcaccggcagccagttgctcagtcaacagacggtgtccgtgg
aaaacgagggatttcaaaaggagtacacgcatttgatataacttgggatggaccgttggg
aactgtagccgttgttggtattgctactaaacatgctgctcttcactgtgttggctatgt
tgctctcctcggatcagatgatcaatcatggggatggaatcttgtagataatgttttgat
gcacaatggagcacagcttggtgtttatccaaaaatgaacaatcctccgaaatatgaggt
cggcgataaaatccgcctaataatcgactgtgacacccatgtcgcttactttgaacgaaa
ctcggaatttctcggaatcgcattcaaccatattcccccattacgtctctatccggctgt
ctgtgcggtttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAGAAGCAGCAGTTTCCGATCCC 434 589 408 Antisense
CTCGCCGAGCTGGGATCTGCAAAAA 434 233 439 Antisense
GCTGAGAGCCTGGTATTTTGCCTGG 112 297 468 Antisense
TCCGTACCAACGGTTTCACTGTTCA 313 595 524 Antisense
GCAGCCAGTTGCTCAGTCAACAGAC 456 313 552 Antisense
TGGACCGTTGGGAACTGTAGCCGTT 74 547 636 Antisense
CACTGTGTTGGCTATGTTGCTCTCC 359 199 691 Antisense
GTTGCTCTCCTCGGATCAGATGATC 519 439 706 Antisense
ATCGACTGTGACACCCATGTCGCTT 262 35 850 Antisense
GGAATCGCATTCAACCATATTCCCC 472 533 901 Antisense
CTATCCGGCTGTCTGTGCGGTTTAT 107 225 936 Antisense
Sequence Source Affymetrix Proprietary Database