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[181583_at]

Description F08G12.4 /REP_DB=WormBase Gene ID /WP=CE03169 /TR=Q19213 /GB=CAA91456.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 53      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 62      6grp_total > 30      4grp_total > 01      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 524
1 2 3 4 range
A 468.6
(-2.6)
P 		433.8
(-3)
P 		581.3
(-1.3)
P 		958.1
(3)
P 		524
B 488.1
(-3)
P 		519.7
(-2.3)
P 		489.3
(-3)
P 		764.9
(3)
P 		277
C 472.3
(-3)
P 		487.8
(-2.7)
P 		487.5
(-2.7)
P 		762.2
(3)
P 		290
avr 476.3
(-3) 		480.4
(-2.9) 		519.4
(-2.3) 		828.4
(3) 		352

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 317
1 2 3 4 5 6 range
A 432
(-2.8)
P 		547.3
(-0.4)
P 		708.6
(3)
P 		558.5
(-0.1)
P 		442.3
(-2.6)
P 		421.8
(-3)
P 		287
B 547.5
(-0.6)
P 		550.9
(-0.5)
P 		679.4
(3)
P 		506.8
(-1.7)
P 		480.1
(-2.5)
P 		461
(-3)
P 		218
C 413
(-2.5)
P 		605.3
(2.3)
P 		448.9
(-1.6)
P 		634.4
(3)
P 		391.6
(-3)
P 		512.8
(-0)
P 		243
avr 464.2
(-2.1) 		567.8
(1.5) 		612.3
(3) 		566.6
(1.4) 		438
(-3) 		465.2
(-2.1) 		174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 170.6
(3)
P 		94.2
(-3)
P 		152
(1.5)
P 		140.2
(0.6)
P 		76
B 155.7
(2.1)
P 		128.4
(-1.2)
P 		113.4
(-3)
P 		163
(3)
P 		50
C 156.3
(1)
P 		140.3
(-1.3)
P 		169.9
(3)
P 		128.5
(-3)
P 		41
avr 160.9
(3) 		121
(-3) 		145.1
(0.6) 		143.9
(0.4) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 79.6
(-3)
P 		194.6
(3)
P 		154.3
(0.9)
P 		141.6
(0.2)
P 		133.5
(-0.2)
P 		102.7
(-1.8)
P 		115
B 190.5
(2.5)
P 		183.4
(1.8)
P 		165
(0)
P 		134.2
(-3)
P 		195.1
(3)
P 		171.9
(0.7)
P 		61
C 153.1
(0.3)
P 		184.7
(3)
P 		114.3
(-3)
P 		127.6
(-1.9)
P 		126.7
(-1.9)
P 		171.7
(1.9)
P 		70
avr 141.1
(-2.3) 		187.6
(3) 		144.5
(-1.9) 		134.5
(-3) 		151.8
(-1) 		148.8
(-1.4) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181583_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17157
Sequence Type Exemplar sequence
Representative Public ID F08G12.4 NCBI
Target Description F08G12.4 /REP_DB=WormBase Gene ID /WP=CE03169 /TR=Q19213 /GB=CAA91456.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077488(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077488 NCBI Caenorhabditis elegans Von Hippel-Lindau tumor suppressor homolog family member (vhl-1) (vhl-1) mRNA, complete cds. 11/11 None
SNAP00000008604 ENSEMBL cdna:SNAP chromosome:CEL140:X:11307293:11308085:1 11/11 None
GENEFINDER00000008624 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11307293:11308085:1 11/11 None
F08G12.4 ENSEMBL cdna:known chromosome:CEL140:X:11307291:11308085:1 gene:F08G12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11307292-11308085(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol vhl-1
Ensembl F08G12.4
Entrez Gene 181321 Entrez gene
SwissProt Q19213 EMBL-EBI
WormBase CE03169 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91456 Hypothetical protein F08G12.4 [Caenorhabditis elegans] ref|NP_509889.1| Von Hippel-Lindau tumor suppressor homolog family member (vhl-1) [Caenorhabditis elegans] 1.0E-101
blast CAE70663 Hypothetical protein CBG17370 [Caenorhabditis briggsae] 1.0E-61
Sequence
Target Sequence 
>C. ELEGANS:181583_AT
tttcctgacttgggctcatcaacacatgacaatcgagagatacgtgttcgtttcctaaac
agatgtgcttaccccgtggacgtgttttggctgaatccatcaaaacaaccgacaaaatac
ggaacacttgcccaaaaaaagtatttagacattaaaactttcaaggatcatccatgggtt
gctaggagatcctttgatggatgcaaagtattggtaaacgaaaaagaagtattctggccg
gagccagctcctcgaatgaatttgattgttcgaaatcattgtgtcatcacaatgaaagtt
caatccttacgagaaattgccggacgttcgttcttacggcataatccaactgaagttcca
aacaaaatcaaaggacttccacgagaactacaatttgaagtcaaacattttctggataga
aaacaagaatattccgaaatagtctgccgttcaattcct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCCTGACTTGGGCTCATCAACAC 698 669 49 Antisense
GAGAGATACGTGTTCGTTTCCTAAA 529 389 83 Antisense
CCTAAACAGATGTGCTTACCCCGTG 592 259 102 Antisense
TACCCCGTGGACGTGTTTTGGCTGA 709 649 118 Antisense
GACAAAATACGGAACACTTGCCCAA 567 363 159 Antisense
CAAGGATCATCCATGGGTTGCTAGG 708 183 210 Antisense
AAGTATTCTGGCCGGAGCCAGCTCC 365 157 275 Antisense
AGCCAGCTCCTCGAATGAATTTGAT 62 85 290 Antisense
GAGAAATTGCCGGACGTTCGTTCTT 488 391 359 Antisense
GTTCGTTCTTACGGCATAATCCAAC 69 443 374 Antisense
CGAAATAGTCTGCCGTTCAATTCCT 250 253 483 Antisense
Sequence Source Affymetrix Proprietary Database