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[181572_s_at]

Description F20A1.7 /REP_DB=WormBase Gene ID /WP=CE27983 /TR=Q19611 /GB=AAA96127.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 18      4grp_total > 53      6grp_cgc > 13      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 100.4
(2)
A 		104.2
(3)
A 		84.9
(-2.2)
A 		82
(-3)
A 		22
B 128.1
(2.9)
A 		129.1
(3)
A 		89.1
(0.6)
A 		30.6
(-3)
A 		99
C 121.9
(3)
A 		73
(-1.9)
A 		62
(-3)
A 		65.2
(-2.7)
A 		60
avr 116.8
(3) 		102.1
(1.5) 		78.7
(-1) 		59.3
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 163.5
(3)
A 		117.8
(1.1)
A 		19.6
(-3)
A 		126.1
(1.4)
A 		62.4
(-1.2)
A 		75
(-0.7)
A 		144
B 138.8
(3)
A 		108
(0.1)
A 		80
(-2.5)
A 		88.8
(-1.6)
A 		74.2
(-3)
A 		81.9
(-2.3)
A 		65
C 160.7
(3)
A 		113
(-0.2)
A 		87.8
(-1.9)
A 		104.8
(-0.8)
A 		103
(-0.9)
A 		71.4
(-3)
A 		89
avr 154.3
(3) 		112.9
(0.3) 		62.5
(-3) 		106.6
(-0.1) 		79.9
(-1.9) 		76.1
(-2.1) 		92
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 36.5
(3)
A 		22.6
(-0.4)
A 		22.2
(-0.5)
A 		12
(-3)
A 		25
B 40.9
(3)
A 		31.9
(1.4)
A 		20.6
(-0.5)
A 		6.5
(-3)
A 		34
C 40.4
(3)
A 		21
(-1)
A 		21.6
(-0.8)
A 		11
(-3)
A 		29
avr 39.3
(3) 		25.2
(0.1) 		21.5
(-0.6) 		9.8
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 30.1
(1.1)
A 		41.9
(3)
A 		4.3
(-3)
A 		32
(1.4)
A 		18.8
(-0.7)
A 		18.3
(-0.8)
A 		38
B 48.3
(3)
A 		36
(0.4)
A 		19.4
(-3)
A 		23.5
(-2.1)
A 		30.2
(-0.8)
A 		30.6
(-0.7)
A 		29
C 59.6
(3)
A 		34.5
(-0.9)
A 		22.3
(-2.8)
A 		21.1
(-3)
A 		33.3
(-1.1)
A 		23.9
(-2.6)
A 		39
avr 46
(3) 		37.5
(1.3) 		15.3
(-3) 		25.5
(-1) 		27.4
(-0.6) 		24.3
(-1.2) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181572_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11561
Sequence Type Exemplar sequence
Representative Public ID F20A1.7 NCBI
Target Description F20A1.7 /REP_DB=WormBase Gene ID /WP=CE27983 /TR=Q19611 /GB=AAA96127.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072382(11), NM_072381(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072382 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-11) (twk-11) mRNA, complete cds. 11/11 None
NM_072381 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-11) (twk-11) mRNA, complete cds. 11/11 None
SNAP00000026800 ENSEMBL cdna:SNAP chromosome:CEL140:V:7031001:7037548:-1 11/11 None
GENEFINDER00000026814 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7031001:7037945:-1 11/11 None
F20A1.7b ENSEMBL cdna:known chromosome:CEL140:V:7030935:7039035:-1 gene:F20A1.7 11/11 None
F20A1.7c ENSEMBL cdna:known chromosome:CEL140:V:7030935:7039035:-1 gene:F20A1.7 11/11 None
F20A1.7a ENSEMBL cdna:known chromosome:CEL140:V:7030948:7039035:-1 gene:F20A1.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182339 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179088 3 2 NM_072381 181572_s_at (11)
NM_072382 181572_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7031001-7037787(-) 97.84 100.0
Public Domain and Genome References
Ensembl F20A1.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504783 TWiK family of potassium channels family member (twk-11) [Caenorhabditis elegans] gb|AAL02489.1| Twik family of potassium channels protein 11, isoform b [Caenorhabditis elegans] 0.0
blast NP_872139 TWiK family of potassium channels family member (twk-11) [Caenorhabditis elegans] gb|AAN84820.1| Twik family of potassium channels protein 11, isoform c [Caenorhabditis elegans] 0.0
blast NP_504782 TWiK family of potassium channels family member (twk-11) [Caenorhabditis elegans] gb|AAA96127.2| Twik family of potassium channels protein 11, isoform a [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504783.1 5 72-94,159-181,193-215,301-323,328-350
NP_504782.1 5 72-94,159-181,193-215,301-323,328-350
F20A1.7b 5 72-94,159-181,193-215,301-323,328-350
F20A1.7c 5 72-94,159-181,193-215,301-323,328-350
F20A1.7a 5 72-94,159-181,193-215,301-323,328-350
SNAP00000026800 5 41-63,128-150,162-184,270-292,297-319
GENEFINDER00000026814 5 90-112,177-199,211-233,319-341,346-368
Sequence
Target Sequence 
>C. ELEGANS:181572_S_AT
aaaggacgtccaggcaacgccaatctcatttgaaactcagacacaacattccattgaact
tgaagtgaaatcttgccaaaccgaaggagttcgaatgaaaaacaaaaagagtcaggtgta
tgttcaagaactcgatgaagaacgtcgaatgcgtatgtcagacattggcatacaaaccgg
tgttctggcacgtgttcaacacatttatacccgtccaatatctgatgatgtgggaccatc
ttcatctgctccaatcactcttcgaaagtcttccgactcctcggaacacatatcaacgaa
aactgcgaaaagcagcgaagagtcattggcgccatccgcttccgggacagcgctctctgc
agctgaatcaatggatgatcatttatggccagagagagcaagtccatttgcatcgaggca
tggctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGGACGTCCAGGCAACGCCAATC 469 119 4047 Antisense
GAAACTCAGACACAACATTCCATTG 528 357 4078 Antisense
GGCATACAAACCGGTGTTCTGGCAC 319 537 4213 Antisense
GTGTTCAACACATTTATACCCGTCC 478 485 4238 Antisense
ATACCCGTCCAATATCTGATGATGT 558 23 4253 Antisense
TGATGTGGGACCATCTTCATCTGCT 501 567 4272 Antisense
TGCTCCAATCACTCTTCGAAAGTCT 301 581 4293 Antisense
CGACTCCTCGGAACACATATCAACG 594 251 4320 Antisense
AGCGAAGAGTCATTGGCGCCATCCG 200 83 4360 Antisense
GCGCTCTCTGCAGCTGAATCAATGG 376 287 4396 Antisense
GTCCATTTGCATCGAGGCATGGCTC 642 475 4448 Antisense
Sequence Source Affymetrix Proprietary Database