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[181571_s_at]

Description Y53F4B.3 /REP_DB=WormBase Gene ID /WP=CE22389 /TR=Q9NAC5 /GB=CAB61070.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 76      4grp_total > 58      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 285
1 2 3 4 range
A 180.5
(-1.1)
A 		221.5
(-0.2)
A 		370.1
(3)
A 		89.4
(-3)
A 		281
B 242.6
(1.2)
A 		308.7
(3)
A 		275
(2.1)
A 		85.5
(-3)
A 		223
C 177.2
(-3)
A 		277.4
(1.8)
A 		301.5
(3)
A 		205.7
(-1.6)
M 		124
avr 200.1
(-0.7) 		269.2
(1.5) 		315.5
(3) 		126.9
(-3) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 246
1 2 3 4 5 6 range
A 261.7
(-2)
A 		236.8
(-3)
A 		386.5
(3)
M 		286.5
(-1)
A 		315.2
(0.1)
A 		376.5
(2.6)
P 		150
B 180.9
(-2.6)
A 		312.3
(2.3)
A 		170.7
(-3)
M 		330.9
(3)
P 		314.7
(2.4)
P 		329.4
(2.9)
P 		160
C 192.6
(-3)
A 		317.5
(0.3)
A 		224.9
(-2.1)
A 		263
(-1.1)
A 		223.3
(-2.2)
A 		416.8
(3)
P 		224
avr 211.7
(-3) 		288.9
(-0.1) 		260.7
(-1.2) 		293.5
(0) 		284.4
(-0.3) 		374.2
(3) 		163
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 65.7
(0.8)
A 		48.1
(-0.5)
A 		96.8
(3)
A 		13.1
(-3)
A 		84
B 77.4
(3)
A 		76.2
(2.9)
A 		63.7
(1.6)
A 		18.2
(-3)
A 		59
C 58.7
(-1)
A 		79.8
(0.8)
A 		105.1
(3)
A 		34.7
(-3)
M 		70
avr 67.3
(1.1) 		68
(1.2) 		88.5
(3) 		22
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 48.2
(-3)
A 		84.2
(1.6)
A 		84.2
(1.6)
M 		72.7
(0.1)
A 		95.1
(3)
A 		91.7
(2.6)
P 		47
B 62.9
(-1.5)
A 		104
(1.3)
A 		41.5
(-3)
M 		87.6
(0.2)
P 		127.9
(3)
P 		122.8
(2.6)
P 		86
C 71.4
(-1.7)
A 		96.9
(0)
A 		57.2
(-2.7)
A 		52.9
(-3)
A 		72.3
(-1.7)
A 		139.5
(3)
P 		87
avr 60.8
(-3) 		95
(0.6) 		61
(-3) 		71.1
(-1.9) 		98.4
(0.9) 		118
(3) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181571_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2919
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.3 NCBI
Target Description Y53F4B.3 /REP_DB=WormBase Gene ID /WP=CE22389 /TR=Q9NAC5 /GB=CAB61070.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064686(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064686 NCBI Caenorhabditis elegans Y53F4B.3 (Y53F4B.3) mRNA, complete cds. 11/11 None
SNAP00000043036 ENSEMBL cdna:SNAP chromosome:CEL140:II:14968591:14969235:-1 11/11 None
GENEFINDER00000043141 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14968591:14969235:-1 11/11 None
Y53F4B.3 ENSEMBL cdna:known chromosome:CEL140:II:14968538:14969244:-1 gene:Y53F4B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14968587-14969232(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.3
Entrez Gene 175152 Entrez gene
SwissProt Q9NAC5 EMBL-EBI
WormBase CE22389 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB61070 Hypothetical protein Y53F4B.3 [Caenorhabditis elegans] ref|NP_497087.1| Y53F4B.3 [Caenorhabditis elegans] 4.0E-63
blast CAE73449 Hypothetical protein CBG20898 [Caenorhabditis briggsae] 1.0E-43
blast AAM50718 GM21739p [Drosophila melanogaster] 5.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR003958 EMBL-EBI Transcription factor CBF/NF-Y/archaeal histone 1.2E-19
Sequence
Target Sequence 
>C. ELEGANS:181571_S_AT
gaaaaccaagaaagccgctccgatcgcgcaaatgagcgaggaggacgtgcgagaaatcga
ggagcacgtcgaagagctcgtccgatcgcaattgccactgggaagagtcaaaaaagtggt
cagaatgaatccggacgtcgagatgctcaacaatgaggcactgcaattgatggcgaaggc
ggcggagctcttcatcaaggagctgagcaacgcggcgaatcagaacgcagcgctggaaaa
acgaaaaaccgtccagacgaaggacattgataaagctatcaagaagacgtgggccttcgc
tttcctggaagatgctctggatgggtggccgaagttggagccaaagaagcgaaaagtgaa
tgcgaattccggtcaggatgagacggttgtagaggaggaaacgattatcgaggaagaaga
ggagatccacgtggaagaagaagaagatgaagagcttccggagaccgttctcgaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAACCAAGAAAGCCGCTCCGATC 195 355 27 Antisense
GCCGCTCCGATCGCGCAAATGAGCG 480 285 40 Antisense
TCCGATCGCAATTGCCACTGGGAAG 331 595 107 Antisense
GAATCCGGACGTCGAGATGCTCAAC 90 331 153 Antisense
ATTGATGGCGAAGGCGGCGGAGCTC 111 3 192 Antisense
CAAGGAGCTGAGCAACGCGGCGAAT 608 181 222 Antisense
GCGGCGAATCAGAACGCAGCGCTGG 102 295 238 Antisense
CAAGAAGACGTGGGCCTTCGCTTTC 85 179 306 Antisense
GCCTTCGCTTTCCTGGAAGATGCTC 83 281 319 Antisense
GATGCTCTGGATGGGTGGCCGAAGT 427 413 337 Antisense
AGCTTCCGGAGACCGTTCTCGAAAT 678 77 479 Antisense
Sequence Source Affymetrix Proprietary Database