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[181554_at]

Description F22D3.4 /REP_DB=WormBase Gene ID /WP=CE02673 /TR=Q19715 /GB=AAK31494.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 76      4grp_total > 18      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 22.3
(-0.8)
A 		35.6
(3)
A 		29.9
(1.4)
A 		14.7
(-3)
A 		21
B 4.7
(-2.4)
A 		1.1
(-3)
A 		6.4
(-2.2)
A 		39.1
(3)
A 		38
C 18.5
(-1.9)
A 		24.6
(-1.1)
A 		10.1
(-3)
A 		55.9
(3)
A 		46
avr 15.2
(-3) 		20.4
(-1.5) 		15.5
(-2.9) 		36.6
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 2.8
(-2.8)
A 		16.6
(-1.5)
A 		15.7
(-1.6)
A 		1.2
(-3)
A 		5.9
(-2.5)
A 		63
(3)
A 		62
B 16.5
(0.6)
A 		2.6
(-3)
A 		11.8
(-0.6)
A 		11.4
(-0.7)
A 		22.2
(2.1)
A 		25.6
(3)
A 		23
C 13.5
(-2.1)
A 		14.5
(-2)
A 		5.4
(-3)
A 		58.4
(3)
A 		20.8
(-1.3)
A 		23.3
(-1)
A 		53
avr 10.9
(-3) 		11.2
(-2.9) 		11
(-3) 		23.7
(-0.1) 		16.3
(-1.8) 		37.3
(3) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 8.1
(3)
A 		7.7
(2.6)
A 		7.8
(2.7)
A 		2.1
(-3)
A 		6
B 1.5
(-2.1)
A 		0.3
(-3)
A 		1.5
(-2.1)
A 		8.3
(3)
A 		8
C 6.1
(-0.4)
A 		7.1
(0.7)
A 		3.5
(-3)
A 		9.4
(3)
A 		6
avr 5.2
(-0.7) 		5
(-1.2) 		4.3
(-3) 		6.6
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 0.5
(-2.9)
A 		5.9
(-0.8)
A 		3.4
(-1.8)
A 		0.3
(-3)
A 		1.8
(-2.4)
A 		15.3
(3)
A 		15
B 5.7
(0.3)
A 		0.9
(-3)
A 		2.9
(-1.6)
A 		3
(-1.6)
A 		9
(2.6)
A 		9.6
(3)
A 		9
C 5
(-0.9)
A 		4.4
(-1.3)
A 		1.4
(-3)
A 		11.7
(3)
A 		6.7
(0.1)
A 		7.8
(0.7)
A 		10
avr 3.7
(-2.2) 		3.7
(-2.2) 		2.6
(-3) 		5
(-1.3) 		5.8
(-0.7) 		10.9
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181554_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4900
Sequence Type Exemplar sequence
Representative Public ID F22D3.4 NCBI
Target Description F22D3.4 /REP_DB=WormBase Gene ID /WP=CE02673 /TR=Q19715 /GB=AAK31494.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063020(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063020 NCBI Caenorhabditis elegans F22D3.4 (F22D3.4) mRNA, complete cds. 11/11 None
GENEFINDER00000022827 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6925033:6926307:-1 9/11 None
F22D3.4 ENSEMBL cdna:known chromosome:CEL140:II:6925033:6925880:-1 gene:F22D3.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022817 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6925028-6925876(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22D3.4
Entrez Gene 184825 Entrez gene
SwissProt Q19715 EMBL-EBI
WormBase CE02673 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31494 Hypothetical protein F22D3.4 [Caenorhabditis elegans] ref|NP_495421.1| F22D3.4 [Caenorhabditis elegans] 5.0E-68
blast AAK31494 Hypothetical protein F22D3.4 [Caenorhabditis elegans] ref|NP_495421.1| F22D3.4 [Caenorhabditis elegans] 2.0E-52
blast CAE65997 Hypothetical protein CBG11188 [Caenorhabditis briggsae] 5.0E-35
blast CAE65997 Hypothetical protein CBG11188 [Caenorhabditis briggsae] 1.0E-27
Sequence
Target Sequence 
>C. ELEGANS:181554_AT
atttatatctactggttctttcgtcaacttttctttgtttagcagctgaggaaatataca
aaaccaatggaaataagggagagaaattcggatcgaaagatggccctgaagctgaaatgg
ttgaaaattcagatatagtagaggaccaaaatgctcaatcgtcagctagatcacttggaa
aaccttattactatgaggaagacggatttttggtcatcgaaaacgccaatgtgtattctc
agggtgtctatttctgtttcgatgaagattctgttgcatcacaacgatttttctatattt
tgattccaattcttccagtatttcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTATATCTACTGGTTCTTTCGTC 261 13 17 Antisense
GTCAACTTTTCTTTGTTTAGCAGCT 651 463 39 Antisense
AATTCGGATCGAAAGATGGCCCTGA 277 175 101 Antisense
GATGGCCCTGAAGCTGAAATGGTTG 535 405 115 Antisense
GGACCAAAATGCTCAATCGTCAGCT 493 521 159 Antisense
GTCAGCTAGATCACTTGGAAAACCT 596 465 177 Antisense
GGATTTTTGGTCATCGAAAACGCCA 91 289 220 Antisense
AACGCCAATGTGTATTCTCAGGGTG 558 145 238 Antisense
AGGGTGTCTATTTCTGTTTCGATGA 231 59 257 Antisense
GAAGATTCTGTTGCATCACAACGAT 661 337 280 Antisense
GATTCCAATTCTTCCAGTATTTCAC 412 431 318 Antisense
Sequence Source Affymetrix Proprietary Database