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[181537_s_at]

Description K12H6.6 /REP_DB=WormBase Gene ID /WP=CE20009 /TR=Q9N5G9 /GB=AAF39936.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 05      4grp_highest_8_8 > 40      4grp_high_8_8 > 42      6grp_highest_nonorm > 75      6grp_total > 04      4grp_total > 53      6grp_highest_sub > De      6grp_high1_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 382
1 2 3 4 range
A 728.4
(3)
P 		494.2
(-2.8)
P 		544.9
(-1.6)
P 		487
(-3)
P 		241
B 655.5
(1.8)
P 		688.9
(3)
P 		595.5
(-0.3)
P 		516.5
(-3)
P 		172
C 678.1
(3)
P 		652.9
(2.5)
P 		554.5
(0.8)
P 		346.3
(-3)
P 		332
avr 687.3
(3) 		612
(1.1) 		565
(-0.1) 		449.9
(-3) 		237

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 286
1 2 3 4 5 6 range
A 885.9
(1.7)
P 		768.3
(-1.9)
P 		864.7
(1.1)
P 		740.5
(-2.7)
P 		926.5
(3)
P 		732.4
(-3)
P 		194
B 1018.4
(3)
P 		857.2
(-1.1)
P 		991.6
(2.3)
P 		923.1
(0.6)
P 		907.4
(0.2)
P 		784
(-3)
P 		234
C 888.9
(0.9)
P 		959.8
(3)
P 		775.1
(-2.4)
P 		769.4
(-2.5)
P 		752.8
(-3)
P 		782.2
(-2.1)
P 		207
avr 931.1
(3) 		861.8
(0.5) 		877.1
(1) 		811
(-1.4) 		862.2
(0.5) 		766.2
(-3) 		165
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 265.2
(3)
P 		107.3
(-1.9)
P 		142.5
(-0.8)
P 		71.3
(-3)
P 		194
B 209
(3)
P 		170.1
(0.6)
P 		138
(-1.3)
P 		110.1
(-3)
P 		99
C 224.5
(3)
P 		187.7
(1.7)
P 		193.2
(1.9)
P 		58.4
(-3)
P 		166
avr 232.9
(3) 		155
(-0.1) 		157.9
(0.1) 		79.9
(-3) 		153

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 163.3
(-3)
P 		273.2
(2.7)
P 		188.3
(-1.7)
P 		187.8
(-1.7)
P 		279.6
(3)
P 		178.3
(-2.2)
P 		116
B 354.4
(2.3)
P 		285.3
(-0.9)
P 		240.8
(-3)
P 		244.5
(-2.8)
P 		368.7
(3)
P 		292.3
(-0.6)
P 		128
C 329.5
(3)
P 		292.9
(1.7)
P 		197.3
(-1.5)
P 		154.7
(-3)
P 		243.6
(0.1)
P 		261.8
(0.7)
P 		175
avr 282.4
(2.1) 		283.8
(2.2) 		208.8
(-2.2) 		195.7
(-3) 		297.3
(3) 		244.1
(-0.1) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181537_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5862
Sequence Type Exemplar sequence
Representative Public ID K12H6.6 NCBI
Target Description K12H6.6 /REP_DB=WormBase Gene ID /WP=CE20009 /TR=Q9N5G9 /GB=AAF39936.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061977(11), NM_182220(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061977 NCBI Caenorhabditis elegans K12H6.6a (K12H6.6) mRNA, complete cds. 11/11 A 
NM_182220 NCBI Caenorhabditis elegans K12H6.6b (K12H6.6) mRNA, complete cds. 11/11 A 
SNAP00000024515 ENSEMBL cdna:SNAP chromosome:CEL140:II:2827612:2831219:-1 11/11 None
GENEFINDER00000024522 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2828762:2831219:-1 11/11 None
K12H6.6a ENSEMBL cdna:known chromosome:CEL140:II:2828582:2831232:-1 gene:K12H6.6 11/11 A 
K12H6.6b ENSEMBL cdna:known chromosome:CEL140:II:2828597:2831219:-1 gene:K12H6.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2828760-2831218(-) 100.0 100.0
Public Domain and Genome References
Ensembl K12H6.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_872020 K12H6.6b [Caenorhabditis elegans] gb|AAO38617.1| Hypothetical protein K12H6.6b [Caenorhabditis elegans] 0.0
blast NP_494378 K12H6.6a [Caenorhabditis elegans] gb|AAF39936.1| Hypothetical protein K12H6.6a [Caenorhabditis elegans] 1.0E-168
blast NP_872020 K12H6.6b [Caenorhabditis elegans] gb|AAO38617.1| Hypothetical protein K12H6.6b [Caenorhabditis elegans] 1.0E-161
blast NP_494378 K12H6.6a [Caenorhabditis elegans] gb|AAF39936.1| Hypothetical protein K12H6.6a [Caenorhabditis elegans] 1.0E-161
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494378.1 4 16-38,164-186,207-229,239-261
NP_872020.1 4 16-38,164-186,207-229,239-261
K12H6.6a 4 16-38,164-186,207-229,239-261
K12H6.6b 4 16-38,164-186,207-229,239-261
SNAP00000024515 4 16-38,164-186,207-229,239-261
GENEFINDER00000024522 4 16-38,164-186,207-229,239-261
Sequence
Target Sequence 
>C. ELEGANS:181537_S_AT
caggaacctggtgctcatgatgactggctacctgctcaacgacctcgtcgacctcatctg
caatgaacgatccgtcagaatcgtggagctcctcttccatcacgtcgtcgtcctctccgc
cttcactatttgcctgttctacgatctcatgctaggcatcgtcgtcgccggactgctcat
ggagctcaactcgatcttcttgcacatcagaagcttgatgaacttatatggattcgacaa
gaagctcaccgcattccgcgttactgtaattctgaacatcatcactctcgtggtgttcag
gctgattgtaaatgtttacatgatctactttgtgttcgcatcgttctcgatgagcccatg
gtacattgcttcaacgatctgcgtgctcattctttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGGAACCTGGTGCTCATGATGACT 405 207 369 Antisense
TCGTCGACCTCATCTGCAATGAACG 408 605 413 Antisense
GATCCGTCAGAATCGTGGAGCTCCT 681 415 437 Antisense
GCCTGTTCTACGATCTCATGCTAGG 417 283 500 Antisense
AACTCGATCTTCTTGCACATCAGAA 569 141 556 Antisense
CGCATTCCGCGTTACTGTAATTCTG 698 253 618 Antisense
ACATCATCACTCTCGTGGTGTTCAG 31 107 644 Antisense
GATCTACTTTGTGTTCGCATCGTTC 639 419 690 Antisense
ATCGTTCTCGATGAGCCCATGGTAC 135 35 708 Antisense
GCCCATGGTACATTGCTTCAACGAT 151 285 722 Antisense
CAACGATCTGCGTGCTCATTCTTTG 496 187 740 Antisense
Sequence Source Affymetrix Proprietary Database