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[181532_at]

Description F13H8.4 /REP_DB=WormBase Gene ID /WP=CE02638 /TR=Q19430 /GB=AAK31486.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 08      4grp_high_8_8 > 60      6grp_highest_nonorm > 80      6grp_total > 09      4grp_total > 59      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 181.3
(-1)
P 		192.9
(-0.4)
P 		260.1
(3)
P 		141.1
(-3)
P 		119
B 163.7
(2.3)
P 		173.5
(3)
P 		172.5
(2.9)
P 		94.4
(-3)
M 		79
C 180.5
(1.7)
P 		196
(3)
P 		171.2
(0.9)
P 		125.5
(-3)
P 		71
avr 175.2
(1.1) 		187.5
(2) 		201.3
(3) 		120.3
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 358
(3)
P 		179.8
(-1.9)
P 		251.1
(0.1)
P 		214.7
(-0.9)
P 		189.8
(-1.6)
P 		137.9
(-3)
P 		220
B 313.2
(3)
P 		191.5
(-1.2)
P 		161.4
(-2.2)
P 		268.2
(1.4)
P 		185.9
(-1.4)
P 		139.4
(-3)
P 		174
C 223.3
(3)
P 		183.2
(0.9)
P 		110.3
(-3)
P 		204.2
(2)
P 		146.6
(-1.1)
P 		147.2
(-1)
P 		113
avr 298.2
(3) 		184.8
(-1.3) 		174.3
(-1.7) 		229
(0.4) 		174.1
(-1.8) 		141.5
(-3) 		157
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 48
1 2 3 4 range
A 66
(2.7)
P 		41.9
(-0.3)
P 		68
(3)
P 		20.7
(-3)
P 		47
B 52.2
(3)
P 		42.9
(1.3)
P 		40
(0.7)
P 		20.1
(-3)
M 		32
C 59.7
(3)
P 		56.3
(2.5)
P 		59.7
(3)
P 		21.2
(-3)
P 		39
avr 59.3
(3) 		47
(1.1) 		55.9
(2.5) 		20.7
(-3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 66
(3)
P 		63.9
(2.6)
P 		54.7
(0.9)
P 		54.4
(0.9)
P 		57.3
(1.4)
P 		33.6
(-3)
P 		32
B 109
(3)
P 		63.7
(-0.9)
P 		39.2
(-3)
P 		71
(-0.3)
P 		75.5
(0.1)
P 		52
(-1.9)
P 		70
C 82.8
(3)
P 		55.9
(0)
P 		28.1
(-3)
P 		41.1
(-1.6)
P 		47.4
(-0.9)
P 		49.3
(-0.7)
P 		55
avr 85.9
(3) 		61.2
(-0.3) 		40.7
(-3) 		55.5
(-1) 		60.1
(-0.4) 		45
(-2.4) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181532_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5832
Sequence Type Exemplar sequence
Representative Public ID F13H8.4 NCBI
Target Description F13H8.4 /REP_DB=WormBase Gene ID /WP=CE02638 /TR=Q19430 /GB=AAK31486.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062852(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062852 NCBI Caenorhabditis elegans F13H8.4 (F13H8.4) mRNA, complete cds. 11/11 None
SNAP00000031303 ENSEMBL cdna:SNAP chromosome:CEL140:II:6261284:6263242:1 11/11 None
GENEFINDER00000031315 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6261173:6262392:1 11/11 None
F13H8.4 ENSEMBL cdna:known chromosome:CEL140:II:6261170:6262392:1 gene:F13H8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6261166-6262389(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F13H8.4
Entrez Gene 184437 Entrez gene
SwissProt Q19430 EMBL-EBI
WormBase CE35476 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31486 Hypothetical protein F13H8.4 [Caenorhabditis elegans] ref|NP_495253.2| F13H8.4 [Caenorhabditis elegans] 1.0E-148
blast AAK31486 Hypothetical protein F13H8.4 [Caenorhabditis elegans] ref|NP_495253.2| F13H8.4 [Caenorhabditis elegans] 1.0E-147
blast AAK31486 Hypothetical protein F13H8.4 [Caenorhabditis elegans] ref|NP_495253.2| F13H8.4 [Caenorhabditis elegans] 1.0E-123
blast CAE59276 Hypothetical protein CBG02608 [Caenorhabditis briggsae] 2.0E-99
blast CAE59276 Hypothetical protein CBG02608 [Caenorhabditis briggsae] 9.0E-99
blast CAE59276 Hypothetical protein CBG02608 [Caenorhabditis briggsae] 1.0E-86
blast CAE59277 Hypothetical protein CBG02609 [Caenorhabditis briggsae] 4.0E-41
blast EAA00445 ENSANGP00000015663 [Anopheles gambiae str. PEST] ref|XP_320513.1| ENSANGP00000015663 [Anopheles gambiae str. PEST] 6.0E-16
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495253.2 3 30-52,73-106,156-178
F13H8.4 3 30-52,73-106,156-178
SNAP00000031303 2 46-68,119-141
GENEFINDER00000031315 3 29-51,72-105,155-177
Sequence
Target Sequence 
>C. ELEGANS:181532_AT
tattatttcttcgatcggctctgcatgaatcttccaggatatacagatggtgactgtata
gatcttcatgttttctggccattagtttcgtcctttgccaactcgaatcttcgaatgtgc
ggtggagatgtgcaacaattctgtgcacttacctccacagcattctcttggtatgtcatc
ggatgggttggatgtgttctcatcattcttggagttcttttattttttggaattcatgca
tcaaattatgcacatattggaaatgctaacagatacgttgaacttggccatcttcggata
gttgaacatcgagaaccaggagcagatcgaaattctaaaaatgcaaatggtcggaaagat
gtacgattgccagaaaaatatgaacttcaatcaacacaatatagtgattctcgtgcaaat
tattcaatgagaacctcaacaagccgtcaagctgagcacatgtctgattctgtcagtcag
ttcgattatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTATTTCTTCGATCGGCTCTGCA 683 661 235 Antisense
TCGGCTCTGCATGAATCTTCCAGGA 465 607 249 Antisense
TTTTCTGGCCATTAGTTTCGTCCTT 480 677 305 Antisense
TCCTTTGCCAACTCGAATCTTCGAA 642 591 325 Antisense
GCAACAATTCTGTGCACTTACCTCC 314 319 366 Antisense
ACCTCCACAGCATTCTCTTGGTATG 620 87 385 Antisense
GTGTTCTCATCATTCTTGGAGTTCT 527 485 428 Antisense
GATACGTTGAACTTGGCCATCTTCG 505 423 506 Antisense
GAACCTCAACAAGCCGTCAAGCTGA 562 345 665 Antisense
GCTGAGCACATGTCTGATTCTGTCA 209 297 685 Antisense
GATTCTGTCAGTCAGTTCGATTATC 34 433 700 Antisense
Sequence Source Affymetrix Proprietary Database