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[181528_at]

Description Y48B6A.4 /REP_DB=WormBase Gene ID /WP=CE22119 /TR=Q9U298 /GB=CAB54450.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 14      4grp_total > 20      6grp_cgc > 21      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 63.8
(-2.6)
P 		101.2
(-0.1)
A 		57.9
(-3)
A 		148.8
(3)
P 		91
B 105.9
(1)
P 		92.8
(-1.3)
A 		83.1
(-3)
A 		117.3
(3)
P 		34
C 105.8
(1.3)
A 		66.2
(-1.4)
A 		43.9
(-3)
A 		129.7
(3)
P 		86
avr 91.8
(-0.4) 		86.7
(-0.9) 		61.6
(-3) 		131.9
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 108.2
(3)
P 		64.3
(-0.1)
A 		76.7
(0.8)
A 		67.1
(0.1)
A 		39.7
(-1.8)
A 		21.9
(-3)
A 		86
B 76.3
(2)
P 		83.2
(3)
P 		72.3
(1.4)
M 		59.5
(-0.4)
A 		41.5
(-3)
A 		45.9
(-2.4)
A 		42
C 129.9
(3)
P 		64.1
(-0.5)
P 		40
(-1.8)
A 		26.6
(-2.5)
A 		23.2
(-2.7)
A 		17.9
(-3)
A 		112
avr 104.8
(3) 		70.5
(0.3) 		63
(-0.3) 		51.1
(-1.2) 		34.8
(-2.5) 		28.6
(-3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 23.2
(3)
P 		22
(2.1)
A 		15.1
(-3)
A 		21.8
(2)
P 		8
B 33.8
(3)
P 		22.9
(-1.5)
A 		19.3
(-3)
A 		25
(-0.6)
P 		15
C 35
(3)
A 		19
(-1.9)
A 		15.3
(-3)
A 		21.9
(-1)
P 		20
avr 30.7
(3) 		21.3
(-1) 		16.6
(-3) 		22.9
(-0.3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 19.9
(2)
P 		22.9
(3)
A 		16.7
(0.9)
A 		17
(1)
A 		12
(-0.7)
A 		5.3
(-3)
A 		18
B 26.5
(2.4)
P 		27.7
(3)
P 		17.6
(-2.1)
M 		15.7
(-3)
A 		16.9
(-2.4)
A 		17.1
(-2.3)
A 		12
C 48.1
(3)
P 		19.5
(-1)
P 		10.2
(-2.3)
A 		5.4
(-3)
A 		7.5
(-2.7)
A 		6
(-2.9)
A 		43
avr 31.5
(3) 		23.4
(0.8) 		14.8
(-1.6) 		12.7
(-2.1) 		12.1
(-2.3) 		9.5
(-3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181528_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2810
Sequence Type Exemplar sequence
Representative Public ID Y48B6A.4 NCBI
Target Description Y48B6A.4 /REP_DB=WormBase Gene ID /WP=CE22119 /TR=Q9U298 /GB=CAB54450.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064558 NCBI Caenorhabditis elegans EATing: abnormal pharyngeal pumping family member (eat-2) (eat-2) mRNA, complete cds. 11/11 None
GENEFINDER00000022986 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14166779:14171375:-1 11/11 None
Y48B6A.4 ENSEMBL cdna:known chromosome:CEL140:II:14166773:14171539:-1 gene:Y48B6A.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022958 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14166775-14171372(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol eat-2
Ensembl Y48B6A.4
Entrez Gene 175072 Entrez gene
SwissProt Q9U298 EMBL-EBI
WormBase CE22119 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
45211 postsynaptic membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4889 nicotinic acetylcholine-activated cation-selective channel activity inferred from electronic annotation QuickGO AmiGO
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5230 extracellular ligand-gated ion channel activity inferred from electronic annotation QuickGO AmiGO
30594 neurotransmitter receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54450 Hypothetical protein Y48B6A.4 [Caenorhabditis elegans] ref|NP_496959.1| EATing: abnormal pharyngeal pumping family member (eat-2) [Caenorhabditis elegans] 0.0
blast CAE73537 Hypothetical protein CBG21000 [Caenorhabditis briggsae] 0.0
blast CAE63105 Hypothetical protein CBG07400 [Caenorhabditis briggsae] 1.0E-107
Protein Domains
Database ID Description E-Value
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 5.1E-19
Pfam IPR006029 EMBL-EBI Neurotransmitter-gated ion-channel transmembrane region 3.0E-6
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.9E-85
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496959.1 4 234-256,268-287,297-319,441-460
Y48B6A.4 4 234-256,268-287,297-319,441-460
GENEFINDER00000022986 4 234-256,268-287,297-319,441-460
Sequence
Target Sequence 
>C. ELEGANS:181528_AT
atccgtgatggtattcctcgctatggtgtccgaagtggctccgcccacttctgagtcaat
tcccataatcgccgcgtttttctccttatctattgtaattctggggctctcgatttgcgc
aagtctcatcatcgtcaacattttcttccggcacccaaagactcatcggatgggcgactg
gacccgctacgtgttccttgaatggcttccatggttcctgttgatgagccgcccggagca
cacattctgcagaccgcggcgagaagaggagaagaacgatgaagaagctgggggtgatgg
cacgaagttgcttgaaaaccagcaacatcagccacgcccacgacttttggtgaatagtca
gttggtgatggactcgacggtaccctacttggaagaaattatcggctacttgaaggtttt
taaggcaaaactcgatgacgacgaggaggaggaagaagagatcctgaactggagattcat
ggcaatggtgatcgatcggctttctctgttcctcttcactggtcttatattcggcaccac
agcacttattttcgcatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCCGTGATGGTATTCCTCGCTATG 40 39 834 Antisense
ATTTGCGCAAGTCTCATCATCGTCA 493 17 946 Antisense
TCATCGTCAACATTTTCTTCCGGCA 307 621 962 Antisense
GACTCATCGGATGGGCGACTGGACC 682 373 993 Antisense
TACGTGTTCCTTGAATGGCTTCCAT 529 641 1021 Antisense
TCCATGGTTCCTGTTGATGAGCCGC 85 591 1041 Antisense
CCACGCCCACGACTTTTGGTGAATA 370 273 1165 Antisense
TGGTGATGGACTCGACGGTACCCTA 606 551 1196 Antisense
GGCAATGGTGATCGATCGGCTTTCT 352 533 1314 Antisense
GTTCCTCTTCACTGGTCTTATATTC 461 441 1341 Antisense
GCACCACAGCACTTATTTTCGCATT 402 315 1367 Antisense
Sequence Source Affymetrix Proprietary Database