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[181521_at]

Description F28F5.3 /REP_DB=WormBase Gene ID /WP=CE24929 /TR=Q19885 /GB=AAA50684.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 20      6grp_cgc > 88      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 95
(-0.4)
A 		113
(0.8)
M 		58.2
(-3)
A 		144.7
(3)
A 		87
B 98.5
(1.5)
A 		74.3
(-3)
A 		103.6
(2.5)
P 		106.4
(3)
A 		32
C 113.5
(-0.5)
A 		116.1
(-0.4)
A 		63.9
(-3)
P 		185.2
(3)
P 		121
avr 102.3
(-0.7) 		101.1
(-0.8) 		75.2
(-3) 		145.4
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 127.7
(0.7)
A 		56.8
(-2.2)
A 		121.9
(0.5)
M 		38.8
(-3)
A 		181.2
(3)
A 		63.4
(-2)
A 		142
B 105.5
(-0.4)
A 		111.2
(0)
A 		68.3
(-3)
A 		153.8
(3)
A 		102.2
(-0.6)
A 		125.2
(1)
A 		86
C 44.2
(-3)
A 		114.4
(2.5)
A 		120.7
(2.9)
A 		115.1
(2.5)
A 		95.4
(1)
P 		121.4
(3)
A 		77
avr 92.5
(-3) 		94.1
(-2.7) 		103.6
(-1) 		102.6
(-1.2) 		126.3
(3) 		103.3
(-1.1) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 34.6
(3)
A 		24.5
(-0.1)
M 		15.2
(-3)
A 		21.2
(-1.1)
A 		19
B 31.4
(3)
A 		18.4
(-3)
A 		24
(-0.4)
P 		22.7
(-1)
A 		13
C 37.6
(3)
A 		33.4
(1.4)
A 		22.3
(-3)
P 		31.2
(0.5)
P 		15
avr 34.5
(3) 		25.4
(-0.9) 		20.5
(-3) 		25
(-1.1) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 23.5
(-1.2)
A 		20.2
(-1.6)
A 		26.5
(-0.8)
M 		9.8
(-3)
A 		54.7
(3)
A 		15.4
(-2.3)
A 		45
B 36.7
(1)
A 		37
(1.1)
A 		16.6
(-3)
A 		40.7
(1.8)
A 		41.5
(2)
A 		46.7
(3)
A 		30
C 16.4
(-3)
A 		34.9
(1.6)
A 		30.7
(0.5)
A 		23.2
(-1.3)
A 		30.9
(0.6)
P 		40.6
(3)
A 		24
avr 25.5
(-2.7) 		30.7
(-0.9) 		24.6
(-3) 		24.6
(-3) 		42.4
(3) 		34.2
(0.2) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181521_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6449
Sequence Type Exemplar sequence
Representative Public ID F28F5.3 NCBI
Target Description F28F5.3 /REP_DB=WormBase Gene ID /WP=CE24929 /TR=Q19885 /GB=AAA50684.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171173(11), NM_066082(11), NM_066083(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171173 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-204) (tag-204) mRNA, complete cds. 11/11 None
NM_066082 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-204) (tag-204) mRNA, complete cds. 11/11 None
NM_066083 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-204) (tag-204) mRNA, complete cds. 11/11 None
GENEFINDER00000021165 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6802732:6815710:1 11/11 None
SNAP00000021159 ENSEMBL cdna:SNAP chromosome:CEL140:III:6812616:6815710:1 11/11 None
F28F5.3c ENSEMBL cdna:known chromosome:CEL140:III:6802720:6816059:1 gene:F28F5.3 11/11 None
F28F5.3a ENSEMBL cdna:known chromosome:CEL140:III:6806464:6816059:1 gene:F28F5.3 11/11 None
F28F5.3b ENSEMBL cdna:known chromosome:CEL140:III:6807430:6816059:1 gene:F28F5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6802412-6815709(+) 100.0 100.0
Public Domain and Genome References
Ensembl F28F5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741201 Temporarily Assigned Gene name family member (tag-204) [Caenorhabditis elegans] gb|AAM29684.1| Temporarily assigned gene name protein 204, isoform c [Caenorhabditis elegans] 0.0
blast NP_498483 Temporarily Assigned Gene name family member (tag-204) [Caenorhabditis elegans] gb|AAL02501.1| Temporarily assigned gene name protein 204, isoform a [Caenorhabditis elegans] 0.0
blast CAE70106 Hypothetical protein CBG16554 [Caenorhabditis briggsae] 0.0
blast NP_498484 Temporarily Assigned Gene name family member (tag-204) [Caenorhabditis elegans] gb|AAL02502.1| Temporarily assigned gene name protein 204, isoform b [Caenorhabditis elegans] 0.0
blast T16204 hypothetical protein F28F5.3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.0E-7
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 1.4E-11
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.0E-7
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 1.4E-11
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.0E-7
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 1.4E-11
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 3.0E-7
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 1.4E-11
Pfam IPR001781 EMBL-EBI Zn-binding protein, LIM 1.4E-11
Sequence
Target Sequence 
>C. ELEGANS:181521_AT
gaatcccgtgattggcgtgatgtcatcaaccagcaacgtcaagctgcgcctggaaactcg
gccgatcgtcgtggtattgcagctacgtcatcgtcgttgagtaacctaccctcatatata
aatcgtcgcatggaaaaagagcgggagagagagattgagtataggagagaccgagaatac
agtatgccaccagctgatcctccggtcatcactgaaaagtacgaacgtgatgaatacagt
cgagtcgaggaaaggacatacccggtaccagttgccagaaataaaatgagaagagaagag
acccagatgcaatcatcactacaacaaatgcatagtcaaagttctcaaaaagacgattct
gtggtagccgttagtggaaagcatcggtgtgctcattgtaacgaggaacttggacgtggt
gctgcaatgatcgtcgaaagcctcaatctattctatcatcttgcatgtttcaaatgttac
gtctgcaaaacttcacttggtagtggagccactggagccgatgttcgtgttcgagatgga
cgcctccactgtcaaacctgttacag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCCCGTGATTGGCGTGATGTCA 82 331 3229 Antisense
CAACCAGCAACGTCAAGCTGCGCCT 82 187 3255 Antisense
GTCGTGGTATTGCAGCTACGTCATC 237 471 3296 Antisense
GTCATCGTCGTTGAGTAACCTACCC 430 465 3315 Antisense
ATACAGTATGCCACCAGCTGATCCT 441 23 3405 Antisense
ATACCCGGTACCAGTTGCCAGAAAT 463 1 3486 Antisense
CGAGGAACTTGGACGTGGTGCTGCA 321 249 3630 Antisense
GTGGTGCTGCAATGATCGTCGAAAG 595 489 3644 Antisense
AAAGCCTCAATCTATTCTATCATCT 233 125 3665 Antisense
GGAGCCGATGTTCGTGTTCGAGATG 190 523 3742 Antisense
GCCTCCACTGTCAAACCTGTTACAG 91 283 3770 Antisense
Sequence Source Affymetrix Proprietary Database