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[181518_at]

Description R10D12.7 /REP_DB=WormBase Gene ID /WP=CE12684 /TR=Q9XVJ8 /GB=CAB03247.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 25      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 49.3
(-1.3)
A 		87.5
(1.6)
A 		27.6
(-3)
A 		106
(3)
A 		78
B 42.5
(-3)
A 		79.7
(0.8)
A 		74.6
(0.3)
A 		100.5
(3)
P 		58
C 27.5
(-3)
A 		72.1
(0.9)
A 		95.3
(3)
A 		70.9
(0.8)
A 		68
avr 39.8
(-3) 		79.8
(1.6) 		65.8
(-0) 		92.5
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 80.5
(2.4)
A 		64
(1.2)
A 		88.9
(3)
P 		6.8
(-3)
A 		41.1
(-0.5)
A 		25
(-1.7)
A 		82
B 84.9
(3)
A 		60.1
(1.1)
A 		60.3
(1.1)
A 		5.7
(-3)
A 		51.4
(0.5)
A 		17.6
(-2.1)
A 		79
C 63.9
(-0)
A 		113.5
(3)
A 		73.6
(0.6)
A 		31
(-2)
A 		14.8
(-3)
A 		22
(-2.6)
A 		99
avr 76.4
(2.7) 		79.2
(3) 		74.3
(2.5) 		14.5
(-3) 		35.8
(-1) 		21.5
(-2.4) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 18
(2.5)
A 		19
(3)
A 		7.2
(-3)
A 		15.5
(1.2)
A 		12
B 13.6
(-3)
A 		19.7
(1.7)
A 		17.3
(-0.2)
A 		21.4
(3)
P 		8
C 9.1
(-3)
A 		20.7
(-0.1)
A 		33.2
(3)
A 		12
(-2.3)
A 		24
avr 13.6
(-3) 		19.8
(3) 		19.2
(2.4) 		16.3
(-0.4) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 14.8
(0.7)
A 		22.8
(3)
A 		19.4
(2)
P 		1.7
(-3)
A 		12.4
(0)
A 		6.1
(-1.7)
A 		21
B 29.5
(3)
A 		20
(1)
A 		14.6
(-0.2)
A 		1.5
(-3)
A 		20.9
(1.2)
A 		6.6
(-1.9)
A 		28
C 23.7
(0.8)
A 		34.6
(3)
A 		18.7
(-0.2)
A 		6.2
(-2.7)
A 		4.8
(-3)
A 		7.4
(-2.5)
A 		30
avr 22.7
(2.2) 		25.8
(3) 		17.6
(0.8) 		3.1
(-3) 		12.7
(-0.5) 		6.7
(-2) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181518_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12827
Sequence Type Exemplar sequence
Representative Public ID R10D12.7 NCBI
Target Description R10D12.7 /REP_DB=WormBase Gene ID /WP=CE12684 /TR=Q9XVJ8 /GB=CAB03247.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074061(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074061 NCBI Caenorhabditis elegans R10D12.7 (R10D12.7) mRNA, complete cds. 9/11 None
SNAP00000021052 ENSEMBL cdna:SNAP chromosome:CEL140:V:13952610:13955235:1 11/11 None
GENEFINDER00000021070 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13952661:13952983:1 9/11 None
R10D12.7 ENSEMBL cdna:known chromosome:CEL140:V:13952610:13952983:1 gene:R10D12.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13952609-13952983(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10D12.7
Entrez Gene 187764 Entrez gene
SwissProt Q9XVJ8 EMBL-EBI
WormBase CE38534 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03247 Hypothetical protein R10D12.7 [Caenorhabditis elegans] ref|NP_506462.2| R10D12.7 [Caenorhabditis elegans] 9.0E-35
blast CAB03247 Hypothetical protein R10D12.7 [Caenorhabditis elegans] ref|NP_506462.2| R10D12.7 [Caenorhabditis elegans] 1.0E-34
blast CAE66185 Hypothetical protein CBG11423 [Caenorhabditis briggsae] 2.0E-27
blast CAE66185 Hypothetical protein CBG11423 [Caenorhabditis briggsae] 3.0E-27
blast CAB03297 Hypothetical protein T06C12.9 [Caenorhabditis elegans] ref|NP_506970.1| T06C12.9 [Caenorhabditis elegans] 2.0E-25
Protein Domains
Database ID Description E-Value
Pfam IPR000612 EMBL-EBI Protein of unknown function UPF0057 1.6E-23
Pfam IPR000612 EMBL-EBI Protein of unknown function UPF0057 1.6E-23
Pfam IPR000612 EMBL-EBI Protein of unknown function UPF0057 1.6E-23
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506462.2 2 27-44,51-73
R10D12.7 2 42-59,66-88
SNAP00000021052 2 42-59,66-88
GENEFINDER00000021070 2 25-42,49-71
Sequence
Target Sequence 
>C. ELEGANS:181518_AT
cttgtttctccgattcacatcagtagcaagttttctatggaaatggcagaagttaatgtg
gtcgctgttcctgaagaaaatagacagacttatctggaaactgataatgatcggttggtt
atggcaataatatggctgattatgccgccaatggcggtattcttcaaatgtcgcggatgt
actaaacatgtatttatcaattttcttctttatcttcttcttgtcctcccagcttacaag
catgctacgtggttctgttttgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTGTTTCTCCGATTCACATCAGTA 623 217 22 Antisense
GATTCACATCAGTAGCAAGTTTTCT 246 431 33 Antisense
GAAGTTAATGTGGTCGCTGTTCCTG 244 337 70 Antisense
ATGTGGTCGCTGTTCCTGAAGAAAA 170 49 77 Antisense
GATAATGATCGGTTGGTTATGGCAA 392 423 124 Antisense
ATGGCTGATTATGCCGCCAATGGCG 57 53 153 Antisense
CGCCAATGGCGGTATTCTTCAAATG 535 255 167 Antisense
ATTCTTCAAATGTCGCGGATGTACT 507 11 180 Antisense
ATGTATTTATCAATTTTCTTCTTTA 666 47 209 Antisense
TCCTCCCAGCTTACAAGCATGCTAC 448 593 245 Antisense
AGCATGCTACGTGGTTCTGTTTTGT 407 77 260 Antisense
Sequence Source Affymetrix Proprietary Database