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[181507_at]

Description C18D11.5 /REP_DB=WormBase Gene ID /WP=CE18516 /TR=Q9XTZ6 /GB=CAB07318.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 18      6grp_cgc > 99      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 7.9
(-3)
A 		8.4
(1.3)
A 		8.6
(3)
A 		8.2
(-0.4)
A 		1
B 7
(-3)
A 		20.4
(1.1)
A 		11.9
(-1.5)
A 		26.4
(3)
A 		19
C 6.7
(-2.8)
A 		7.3
(-2.7)
A 		5.5
(-3)
A 		46.2
(3)
A 		41
avr 7.2
(-3) 		12
(-1.5) 		8.7
(-2.5) 		26.9
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 4.2
(-2.6)
A 		5
(-2.5)
A 		16.1
(-0.5)
A 		2
(-3)
A 		36.2
(3)
A 		2.9
(-2.8)
A 		34
B 9.8
(-1.6)
A 		27.2
(3)
A 		13.4
(-0.6)
A 		11.1
(-1.2)
A 		17.1
(0.3)
A 		4.4
(-3)
A 		23
C 7.5
(-1.6)
A 		9.9
(-0.9)
A 		3.8
(-2.5)
A 		5.9
(-2)
A 		2.1
(-3)
A 		24.6
(3)
A 		23
avr 7.2
(-2.6) 		14
(0.8) 		11.1
(-0.6) 		6.3
(-3) 		18.5
(3) 		10.6
(-0.9) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 2.9
(3)
A 		1.8
(-0.9)
A 		2.2
(0.5)
A 		1.2
(-3)
A 		2
B 2.2
(-3)
A 		5
(1.9)
A 		2.7
(-2.1)
A 		5.6
(3)
A 		3
C 2.2
(-2.7)
A 		2.1
(-2.8)
A 		1.9
(-3)
A 		7.8
(3)
A 		6
avr 2.4
(-2.8) 		3
(-1.4) 		2.3
(-3) 		4.9
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 0.8
(-2.8)
A 		1.8
(-2.3)
A 		3.5
(-1.3)
A 		0.5
(-3)
A 		10.9
(3)
A 		0.7
(-2.9)
A 		10
B 3.4
(-1.6)
A 		9
(3)
A 		3.3
(-1.7)
A 		2.9
(-2)
A 		6.9
(1.3)
A 		1.7
(-3)
A 		7
C 2.8
(-1.3)
A 		3
(-1.2)
A 		1
(-2.8)
A 		1.2
(-2.6)
A 		0.7
(-3)
A 		8.2
(3)
A 		8
avr 2.3
(-2) 		4.6
(1) 		2.6
(-1.6) 		1.5
(-3) 		6.2
(3) 		3.5
(-0.4) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181507_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6075
Sequence Type Exemplar sequence
Representative Public ID C18D11.5 NCBI
Target Description C18D11.5 /REP_DB=WormBase Gene ID /WP=CE18516 /TR=Q9XTZ6 /GB=CAB07318.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067134(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067134 NCBI Caenorhabditis elegans NOT-Like (yeast CCR4/NOT complex component) family member (ntl-3) (ntl-3) mRNA, complete cds. 11/11 None
Y56A3A.1 ENSEMBL cdna:known chromosome:CEL140:III:11831345:11846337:-1 gene:Y56A3A.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11831340-11833569(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ntl-3
Ensembl Y56A3A.1
Entrez Gene 176614 Entrez gene
SwissProt Q9U233 EMBL-EBI
WormBase CE33892 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250035_AT transcription regulator NOT2/NOT3/NOT5 family protein at
CANINE_2:CFAAFFX.4925.1.S1_S_AT similar to CCR4-NOT transcription complex, subunit 3 cfa
DROSOPHILA_2:1634881_AT dm
DROSGENOME1:153791_AT dm
HG-U95AV2:38310_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133_PLUS_2:211141_S_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133A:211141_S_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133A_2:211141_S_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133_PLUS_2:203239_S_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133A:203239_S_AT CCR4-NOT transcription complex, subunit 3 hs
HG-FOCUS:203239_S_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133A_2:203239_S_AT CCR4-NOT transcription complex, subunit 3 hs
HU35KSUBC:RC_W68171_AT CCR4-NOT transcription complex, subunit 3 hs
HU35KSUBC:RC_T79588_F_AT CCR4-NOT transcription complex, subunit 3 hs
HU35KSUBC:RC_N34048_F_AT CCR4-NOT transcription complex, subunit 3 hs
HU35KSUBD:RC_W31863_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:G7657386_3P_X_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:G7657386_3P_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:G7657386_3P_A_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:G6856204_3P_X_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:G6856204_3P_A_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:HS.108300.2.A1_3P_X_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:HS.108300.2.A1_3P_AT CCR4-NOT transcription complex, subunit 3 hs
U133_X3P:203239_3P_S_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133_PLUS_2:229143_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U133B:229143_AT CCR4-NOT transcription complex, subunit 3 hs
HG-U95C:61905_AT CCR4-NOT transcription complex, subunit 3 hs
MOE430A:1435965_AT CCR4-NOT transcription complex, subunit 3 mm
MOUSE430A_2:1435965_AT CCR4-NOT transcription complex, subunit 3 mm
MOUSE430_2:1435965_AT CCR4-NOT transcription complex, subunit 3 mm
MG-U74AV2:102018_AT CCR4-NOT transcription complex, subunit 3 mm
MOE430B:1441465_AT CCR4-NOT transcription complex, subunit 3 mm
MOUSE430_2:1441465_AT CCR4-NOT transcription complex, subunit 3 mm
RAE230B:1385720_AT CCR4-NOT transcription complex, subunit 3 (predicted) rn
RAT230_2:1385720_AT CCR4-NOT transcription complex, subunit 3 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
45449 regulation of transcription inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
30528 transcription regulator activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60507 Hypothetical protein Y56A3A.1 [Caenorhabditis elegans] emb|CAD90172.1| Hypothetical protein Y56A3A.1 [Caenorhabditis elegans] ref|NP_499535.2| NOT-Like (yeast CCR4/NOT complex component) family member (ntl-3) [Caenorhabditis elegans] 0.0
blast CAE67726 Hypothetical protein CBG13301 [Caenorhabditis briggsae] 1.0E-176
blast T19385 hypothetical protein C18D11.5 - Caenorhabditis elegans 4.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR007207 EMBL-EBI Not CCR4-Not complex component, N-terminal 1.0E-126
Pfam IPR007282 EMBL-EBI NOT2/NOT3/NOT5 3.1E-43
Sequence
Target Sequence 
>C. ELEGANS:181507_AT
tgatgtcaactgacgatccggcggcggcactgcaagctgcactcaatatggctgctgctt
ctcaacaggctgtcgccaccggaccaaaacgagctcatatcccggcgtggttgggcgctt
caccactcggacgtacctcgatgacacaggagtttgacggacagcttgccgcattggagt
tggcgtgtgctaaggcaacgttcccgttggatagtgagaagccgaggaactacttatcga
aggtctcgttcccggtgccaagttggtatggacagaccgcaccaaacacatcggacagct
tggaatactatctccgcttggctcctgacacgttattcttcattttctactatatggaag
gaacacgtgcccagctgctggcggcaaaggctctcaaaaagctctcgtggcgttttcaca
cgaaatatttgacatggttccagcgacacgaggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATGTCAACTGACGATCCGGCGGC 454 565 26 Antisense
GCTGCACTCAATATGGCTGCTGCTT 64 297 61 Antisense
CTCGGACGTACCTCGATGACACAGG 68 233 151 Antisense
TTGACGGACAGCTTGCCGCATTGGA 401 687 179 Antisense
TGGCGTGTGCTAAGGCAACGTTCCC 243 545 206 Antisense
TACTTATCGAAGGTCTCGTTCCCGG 576 581 256 Antisense
GACCGCACCAAACACATCGGACAGC 702 381 300 Antisense
GCTTGGAATACTATCTCCGCTTGGC 306 303 323 Antisense
GCTCCTGACACGTTATTCTTCATTT 238 299 346 Antisense
AAGGCTCTCAAAAAGCTCTCGTGGC 527 161 412 Antisense
GACATGGTTCCAGCGACACGAGGAG 665 369 456 Antisense
Sequence Source Affymetrix Proprietary Database