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[181504_at]

Description Y53F4B.8 /REP_DB=WormBase Gene ID /WP=CE25407 /TR=Q9NAA6 /GB=CAB70103.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 38      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 9
(1.9)
A 		6
(-1.7)
A 		4.9
(-3)
A 		9.9
(3)
A 		5
B 43.3
(3)
A 		6.5
(-2.5)
A 		3.4
(-3)
A 		8
(-2.3)
A 		40
C 31
(2.6)
A 		27.1
(1.7)
A 		6.4
(-3)
A 		32.7
(3)
A 		26
avr 27.8
(3) 		13.2
(-0.8) 		4.9
(-3) 		16.9
(0.1) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 11.9
(-2.3)
A 		23.7
(-1.1)
A 		6.1
(-2.8)
A 		4.1
(-3)
A 		6.8
(-2.7)
A 		66.5
(3)
A 		62
B 8
(-2)
A 		3.9
(-2.7)
A 		6.9
(-2.2)
A 		6.9
(-2.2)
A 		2
(-3)
A 		37.1
(3)
A 		35
C 3.5
(-2.8)
A 		9.2
(-1.3)
A 		4.3
(-2.6)
A 		25.1
(3)
A 		2.9
(-3)
A 		7.2
(-1.8)
A 		22
avr 7.8
(-2.3) 		12.3
(-1.5) 		5.8
(-2.7) 		12
(-1.5) 		3.9
(-3) 		36.9
(3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 3.3
(3)
A 		1.3
(-3)
A 		1.3
(-3)
A 		1.5
(-2.4)
A 		2
B 13.8
(3)
A 		1.6
(-2.6)
A 		0.8
(-3)
A 		1.7
(-2.6)
A 		13
C 10.3
(3)
A 		7.8
(1.1)
A 		2.2
(-3)
A 		5.5
(-0.6)
A 		8
avr 9.1
(3) 		3.6
(-1.3) 		1.4
(-3) 		2.9
(-1.8) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 2.2
(-2.5)
A 		8.4
(-0.1)
A 		1.3
(-2.9)
A 		1
(-3)
A 		2.1
(-2.6)
A 		16.2
(3)
A 		15
B 2.8
(-2.1)
A 		1.3
(-2.8)
A 		1.7
(-2.6)
A 		1.8
(-2.5)
A 		0.8
(-3)
A 		13.8
(3)
A 		13
C 1.3
(-2.4)
A 		2.8
(-0.3)
A 		1.1
(-2.7)
A 		5.1
(3)
A 		0.9
(-3)
A 		2.4
(-0.9)
A 		4
avr 2.1
(-2.5) 		4.2
(-1.2) 		1.4
(-2.9) 		2.6
(-2.2) 		1.3
(-3) 		10.8
(3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181504_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3607
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.8 NCBI
Target Description Y53F4B.8 /REP_DB=WormBase Gene ID /WP=CE25407 /TR=Q9NAA6 /GB=CAB70103.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064692(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064692 NCBI Caenorhabditis elegans Y53F4B.8 (Y53F4B.8) mRNA, complete cds. 11/11 None
SNAP00000043044 ENSEMBL cdna:SNAP chromosome:CEL140:II:14992788:14997334:1 11/11 None
Y53F4B.8 ENSEMBL cdna:known chromosome:CEL140:II:14996699:14997334:1 gene:Y53F4B.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000043128 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14996695-14997331(+) 83.81 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.8
Entrez Gene 190211 Entrez gene
SwissProt Q9NAA6 EMBL-EBI
WormBase CE25407 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68494 Hypothetical protein Y48G1BM.2 [Caenorhabditis elegans] ref|NP_490704.1| Y48G1BM.2 [Caenorhabditis elegans] 1.0E-113
blast AAK68496 Hypothetical protein Y48G1BM.4 [Caenorhabditis elegans] ref|NP_490700.1| Y48G1BM.4 [Caenorhabditis elegans] 1.0E-113
blast CAB70103 Hypothetical protein Y53F4B.8 [Caenorhabditis elegans] ref|NP_497093.1| Y53F4B.8 [Caenorhabditis elegans] 6.0E-96
blast CAB70103 Hypothetical protein Y53F4B.8 [Caenorhabditis elegans] ref|NP_497093.1| Y53F4B.8 [Caenorhabditis elegans] 3.0E-95
blast AAK68496 Hypothetical protein Y48G1BM.4 [Caenorhabditis elegans] ref|NP_490700.1| Y48G1BM.4 [Caenorhabditis elegans] 2.0E-59
blast AAK68494 Hypothetical protein Y48G1BM.2 [Caenorhabditis elegans] ref|NP_490704.1| Y48G1BM.2 [Caenorhabditis elegans] 2.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR009497 EMBL-EBI Protein of unknown function DUF1114 7.3E-60
Pfam IPR009497 EMBL-EBI Protein of unknown function DUF1114 7.9E-20
Pfam IPR009497 EMBL-EBI Protein of unknown function DUF1114 1.1E-28
Pfam IPR009497 EMBL-EBI Protein of unknown function DUF1114 7.9E-20
Sequence
Target Sequence 
>C. ELEGANS:181504_AT
ggaacttcccgagcaatagtctgacggtactctttcctaaaactcatcttctcatttcac
cggaaaatatgggtagggtcgctgcaaaacttatcaggctcactgaaaagaaccaacaga
gaaatggaattgttacattcaaattgtactatcagcacgaagaaggagaggaagaagagg
aggaagaggtagatgaggacgaggacgaggaaggggaagagggagaggaagaggaagaag
aagaagacgaggtagaagaagttccccagaaaatctcagagattttcaatttaggagccc
atcttgtaggagaaccacctgagaattgttggtatctcgaagacattctcactatcccca
aagaaatcatcaaagacgcatttggagatgtaactgtctggtatggaaaaagtttccaat
tgaggaattcaatagcaaaccggagaattcacttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACTTCCCGAGCAATAGTCTGAC 66 529 89 Antisense
AATAGTCTGACGGTACTCTTTCCTA 440 173 103 Antisense
GGTACTCTTTCCTAAAACTCATCTT 82 503 114 Antisense
CTAAAACTCATCTTCTCATTTCACC 412 227 125 Antisense
GGGTAGGGTCGCTGCAAAACTTATC 43 493 159 Antisense
GAAGTTCCCCAGAAAATCTCAGAGA 571 337 346 Antisense
GATTTTCAATTTAGGAGCCCATCTT 622 427 369 Antisense
GAGCCCATCTTGTAGGAGAACCACC 570 385 383 Antisense
ATCTCGAAGACATTCTCACTATCCC 94 33 422 Antisense
CTCACTATCCCCAAAGAAATCATCA 258 229 436 Antisense
ATAGCAAACCGGAGAATTCACTTCA 152 11 520 Antisense
Sequence Source Affymetrix Proprietary Database