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[181499_s_at]

Description F26F4.5 /REP_DB=WormBase Gene ID /WP=CE01262 /TR=Q19816 /GB=AAA91220.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 97      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_highest_nonorm > 92      6grp_total > 97      4grp_total > 44      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 167.5
(-1.8)
P 		223
(3)
P 		153
(-3)
P 		195.4
(0.6)
P 		70
B 215.5
(1.2)
P 		244.8
(3)
P 		193.9
(-0.2)
P 		148.9
(-3)
P 		96
C 226
(-0.3)
P 		277.1
(3)
P 		188
(-2.7)
P 		183.4
(-3)
P 		94
avr 203
(-0.8) 		248.3
(3) 		178.3
(-2.8) 		175.9
(-3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 341
1 2 3 4 5 6 range
A 132.7
(-3)
P 		239.1
(-0.9)
P 		268
(-0.3)
P 		235.9
(-0.9)
P 		383
(2)
P 		432.3
(3)
P 		300
B 216.7
(-3)
P 		266.6
(-1.8)
P 		285.9
(-1.3)
P 		320.9
(-0.5)
P 		366.3
(0.6)
P 		465.9
(3)
P 		249
C 225.8
(-1.9)
P 		213
(-2.1)
P 		167.1
(-3)
P 		207.4
(-2.2)
P 		368.9
(0.9)
P 		474.1
(3)
P 		307
avr 191.7
(-3) 		239.6
(-1.9) 		240.3
(-1.9) 		254.7
(-1.6) 		372.7
(1.1) 		457.4
(3) 		266
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 61
(3)
P 		48.4
(0.7)
P 		40
(-0.9)
P 		28.6
(-3)
P 		32
B 68.7
(3)
P 		60.5
(1.7)
P 		44.9
(-0.9)
P 		31.7
(-3)
P 		37
C 74.8
(2.4)
P 		79.7
(3)
P 		65.5
(1.3)
P 		30.9
(-3)
P 		49
avr 68.2
(3) 		62.9
(2.2) 		50.1
(0.1) 		30.4
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 24.5
(-3)
P 		85
(1)
P 		58.4
(-0.8)
P 		59.8
(-0.7)
P 		115.6
(3)
P 		105.3
(2.3)
P 		91
B 75.4
(-2.7)
P 		88.7
(-1.9)
P 		69.4
(-3)
P 		85
(-2.1)
P 		148.8
(1.6)
P 		173.7
(3)
P 		104
C 83.7
(-0.8)
P 		65
(-1.8)
P 		42.5
(-3)
P 		41.7
(-3)
P 		119.4
(1)
P 		158.7
(3)
P 		117
avr 61.2
(-2.7) 		79.6
(-1.5) 		56.8
(-3) 		62.2
(-2.6) 		127.9
(1.8) 		145.9
(3) 		89
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181499_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6060
Sequence Type Exemplar sequence
Representative Public ID F26F4.5 NCBI
Target Description F26F4.5 /REP_DB=WormBase Gene ID /WP=CE01262 /TR=Q19816 /GB=AAA91220.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065623(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065623 NCBI Caenorhabditis elegans F26F4.5 (F26F4.5) mRNA, complete cds. 11/11 None
SNAP00000005747 ENSEMBL cdna:SNAP chromosome:CEL140:III:4888158:4892355:-1 11/11 None
GENEFINDER00000005764 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4888158:4893354:-1 11/11 None
F26F4.5.1 ENSEMBL cdna:known chromosome:CEL140:III:4887882:4890912:-1 gene:F26F4.5 11/11 A 
F26F4.5.2 ENSEMBL cdna:known chromosome:CEL140:III:4888158:4891213:-1 gene:F26F4.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4888157-4892228(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F26F4.5
Entrez Gene 175653 Entrez gene
SwissProt Q19816 EMBL-EBI
WormBase CE29289 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL02497 Hypothetical protein F26F4.5 [Caenorhabditis elegans] ref|NP_498024.1| F26F4.5 [Caenorhabditis elegans] 0.0
blast T16167 hypothetical protein F26F4.5 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 6.4E-14
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 6.4E-14
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 6.4E-14
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000005764 1 297-319
Sequence
Target Sequence 
>C. ELEGANS:181499_S_AT
gaggaattccgtcaaactcgcaaggatttcattttaattctcaaggaagctgttttgcgg
aaggatgaagctgaacgtcaattggaaactatttcaaaaagcatatacgatgaagatgac
tgggttaatctgacgtcacagcataagaatacccttcgaagaaatgtacttctcatgtgg
gtacaggaaaaactagaactctactcggatcaattgacagtttccaacttcagttctgac
tggactgattgccgtgctttctgtgctcttctctatgatctcttcccagatacaatgcca
gatgtaactgtttctccaattgttggtgactgcatttctcgttgccgtcgtacttttcaa
cttcttgaaattccattcgatgaacgtgctcttggaatttcttcgccaactgctgattct
ggagcagatgaggattcgtcttcagcttgcagtgtaattactggactcaccgattggcgt
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGAATTCCGTCAAACTCGCAAGG 545 403 1351 Antisense
GACTGGGTTAATCTGACGTCACAGC 253 375 1468 Antisense
GAACTCTACTCGGATCAATTGACAG 676 349 1546 Antisense
GTTCTGACTGGACTGATTGCCGTGC 284 443 1583 Antisense
TTCTCTATGATCTCTTCCCAGATAC 524 691 1619 Antisense
ATGCCAGATGTAACTGTTTCTCCAA 453 41 1645 Antisense
ATTGTTGGTGACTGCATTTCTCGTT 195 5 1669 Antisense
AATTCCATTCGATGAACGTGCTCTT 637 175 1719 Antisense
GCTCTTGGAATTTCTTCGCCAACTG 394 297 1738 Antisense
AGATGAGGATTCGTCTTCAGCTTGC 543 67 1776 Antisense
ATTACTGGACTCACCGATTGGCGTT 512 11 1807 Antisense
Sequence Source Affymetrix Proprietary Database