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[181489_at]

Description C13A2.5 /REP_DB=WormBase Gene ID /WP=CE08117 /TR=O16873 /GB=AAB69877.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 08      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 9.7
(3)
A 		7.2
(-0.3)
A 		6.2
(-1.7)
A 		5.2
(-3)
A 		5
B 6.4
(-3)
A 		8.3
(-2.6)
A 		36.1
(3)
A 		9.4
(-2.4)
A 		30
C 6.8
(-2.8)
A 		5.5
(-2.9)
A 		5.1
(-3)
A 		50.5
(3)
A 		45
avr 7.6
(-2.8) 		7
(-3) 		15.8
(0.6) 		21.7
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 29.2
(-1.9)
A 		19.1
(-2.3)
A 		3.5
(-3)
A 		140.6
(3)
A 		64.7
(-0.3)
A 		9
(-2.8)
A 		137
B 5.1
(-3)
A 		6.1
(-2.9)
A 		6.8
(-2.9)
A 		59.2
(0.3)
A 		103
(3)
A 		8.2
(-2.8)
A 		98
C 40.8
(-1.1)
A 		4.2
(-3)
A 		5.6
(-2.9)
A 		117.3
(3)
A 		65.6
(0.3)
A 		48.5
(-0.6)
A 		113
avr 25
(-1.8) 		9.8
(-2.7) 		5.3
(-3) 		105.7
(3) 		77.8
(1.3) 		21.9
(-2) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 3.5
(3)
A 		1.6
(-1.2)
A 		1.6
(-1.2)
A 		0.8
(-3)
A 		3
B 2
(-3)
A 		2
(-3)
A 		8.4
(3)
A 		2
(-3)
A 		6
C 2.2
(-2.5)
A 		1.6
(-3)
A 		1.8
(-2.8)
A 		8.5
(3)
A 		7
avr 2.6
(-0.5) 		1.7
(-3) 		3.9
(3) 		3.8
(2.7) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 5.4
(-2.2)
A 		6.8
(-2)
A 		0.8
(-3)
A 		35.7
(3)
A 		19.5
(0.2)
A 		2.2
(-2.8)
A 		35
B 1.8
(-3)
A 		2
(-3)
A 		1.7
(-3)
A 		15.7
(-0.9)
A 		41.9
(3)
A 		3.1
(-2.8)
A 		40
C 15.1
(0.7)
A 		1.3
(-3)
A 		1.4
(-3)
A 		23.6
(3)
A 		21.2
(2.4)
A 		16.2
(1)
A 		22
avr 7.4
(-1.6) 		3.4
(-2.5) 		1.3
(-3) 		25
(2.4) 		27.5
(3) 		7.2
(-1.6) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181489_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11621
Sequence Type Exemplar sequence
Representative Public ID C13A2.5 NCBI
Target Description C13A2.5 /REP_DB=WormBase Gene ID /WP=CE08117 /TR=O16873 /GB=AAB69877.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072448(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072448 NCBI Caenorhabditis elegans C13A2.5 (C13A2.5) mRNA, complete cds. 11/11 A 
GENEFINDER00000044278 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:7279140:7285954:1 10/11 None
C13A2.5 ENSEMBL cdna:known chromosome:CEL140:V:7278445:7281037:1 gene:C13A2.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000044271 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7278445-7281038(+) 99.66 100.0
Public Domain and Genome References
Gene Title
Ensembl C13A2.5
Entrez Gene 182553 Entrez gene
SwissProt O16873 EMBL-EBI
WormBase CE08117 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB69877 Hypothetical protein C13A2.5 [Caenorhabditis elegans] ref|NP_504849.1| C13A2.5 [Caenorhabditis elegans] 0.0
blast AAB69877 Hypothetical protein C13A2.5 [Caenorhabditis elegans] ref|NP_504849.1| C13A2.5 [Caenorhabditis elegans] 1.0E-177
blast T19496 hypothetical protein C27A7.2 - Caenorhabditis elegans 1.0E-140
blast AAB69876 Hypothetical protein C13A2.4 [Caenorhabditis elegans] ref|NP_504850.1| C13A2.4 [Caenorhabditis elegans] 1.0E-137
blast AAY43981 Hypothetical protein ZK381.8 [Caenorhabditis elegans] ref|NP_001023614.1| ZK381.8 [Caenorhabditis elegans] 1.0E-130
blast T19496 hypothetical protein C27A7.2 - Caenorhabditis elegans 1.0E-117
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 5.0E-99
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 5.4E-36
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 3.0E-59
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 5.5E-115
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504849.1 1 35-57
C13A2.5 1 35-57
Sequence
Target Sequence 
>C. ELEGANS:181489_AT
ggagcacatgcagactgtttgttacagtataaggaagcggcacaatttatttcatttttt
gatatggacgatattctggtcccattgaactatccgacctattttcaagagttcactcat
gaattcaataaaaatcccggtgctcgatcgattttttatggaagaagggagcacagacag
gcattcactttaacaggtggcccgttgataacagttccatcaataatccatgtgcagcgg
ccgattggaaaagatggtccttcggatgttcatcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGCACATGCAGACTGTTTGTTAC 160 519 841 Antisense
GGAAGCGGCACAATTTATTTCATTT 184 531 873 Antisense
GACGATATTCTGGTCCCATTGAACT 139 379 907 Antisense
CCCATTGAACTATCCGACCTATTTT 228 241 921 Antisense
GACCTATTTTCAAGAGTTCACTCAT 562 383 936 Antisense
ATTCAATAAAAATCCCGGTGCTCGA 639 1 963 Antisense
ATCCCGGTGCTCGATCGATTTTTTA 235 39 974 Antisense
GGGAGCACAGACAGGCATTCACTTT 300 495 1007 Antisense
GCATTCACTTTAACAGGTGGCCCGT 178 307 1021 Antisense
GTGGCCCGTTGATAACAGTTCCATC 692 491 1037 Antisense
GATGGTCCTTCGGATGTTCATCAAC 17 409 1093 Antisense
Sequence Source Affymetrix Proprietary Database