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[181478_s_at]

Description F53B1.2 /REP_DB=WormBase Gene ID /WP=CE04642 /TR=Q20696 /GB=AAB52647.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 93      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 91      6grp_total > 95      4grp_total > 22      6grp_high1_sub > 04      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 244.4
(3)
P 		126.6
(-3)
P 		140.5
(-2.3)
P 		194.3
(0.4)
P 		118
B 161.3
(-1.3)
P 		149.4
(-1.9)
P 		127.3
(-3)
P 		244.7
(3)
P 		117
C 253.6
(3)
P 		175.9
(-1.8)
P 		156.6
(-3)
P 		228.2
(1.4)
P 		97
avr 219.8
(2.8) 		150.6
(-2.3) 		141.5
(-3) 		222.4
(3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 134
1 2 3 4 5 6 range
A 247.5
(1)
P 		171.6
(-3)
P 		208.3
(-1)
P 		215
(-0.7)
P 		284.3
(3)
P 		249
(1.1)
P 		113
B 263.2
(3)
P 		199.3
(-2.7)
P 		195.6
(-3)
P 		223.7
(-0.5)
P 		223.5
(-0.5)
P 		207.3
(-2)
P 		68
C 224.2
(-1.3)
P 		191.7
(-3)
P 		230.7
(-1)
P 		305.9
(3)
P 		236.6
(-0.6)
P 		245
(-0.2)
P 		114
avr 245
(2.7) 		187.5
(-3) 		211.5
(-0.6) 		248.2
(3) 		248.1
(3) 		233.8
(1.6) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 89
(3)
P 		27.5
(-3)
P 		36.7
(-2.1)
P 		28.4
(-2.9)
P 		62
B 51.4
(2.8)
P 		36.9
(-1)
P 		29.5
(-3)
P 		52.1
(3)
P 		23
C 84
(3)
P 		50.6
(-1.4)
P 		54.6
(-0.9)
P 		38.5
(-3)
P 		46
avr 74.8
(3) 		38.3
(-3) 		40.3
(-2.7) 		39.7
(-2.8) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 45.6
(-3)
P 		61
(-0.7)
P 		45.4
(-3)
P 		54.5
(-1.6)
P 		85.8
(3)
P 		60.6
(-0.7)
P 		40
B 91.6
(3)
P 		66.3
(-0.4)
P 		47.5
(-3)
P 		59.2
(-1.4)
P 		90.8
(2.9)
P 		77.3
(1.1)
P 		44
C 83.1
(3)
P 		58.5
(-3)
P 		58.7
(-3)
P 		61.5
(-2.3)
P 		76.5
(1.4)
P 		82
(2.7)
P 		25
avr 73.4
(1.1) 		61.9
(-1) 		50.5
(-3) 		58.4
(-1.6) 		84.4
(3) 		73.3
(1) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181478_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18563
Sequence Type Exemplar sequence
Representative Public ID F53B1.2 NCBI
Target Description F53B1.2 /REP_DB=WormBase Gene ID /WP=CE04642 /TR=Q20696 /GB=AAB52647.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075988(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075988 NCBI Caenorhabditis elegans F53B1.2 (F53B1.2) mRNA, complete cds. 11/11 None
SNAP00000022989 ENSEMBL cdna:SNAP chromosome:CEL140:X:2108976:2111151:1 11/11 None
GENEFINDER00000023001 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2108976:2111151:1 11/11 None
F53B1.2.2 ENSEMBL cdna:known chromosome:CEL140:X:2108974:2111151:1 gene:F53B1.2 11/11 None
F53B1.2.1 ENSEMBL cdna:known chromosome:CEL140:X:2108976:2111423:1 gene:F53B1.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2108975-2111151(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53B1.2
Entrez Gene 180524 Entrez gene
SwissProt Q20696 EMBL-EBI
WormBase CE04642 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23630.1.S1_AT similar to sterile alpha motif domain containing 8 (predicted) cfa
DROSOPHILA_2:1626584_A_AT dm
DROSGENOME1:148329_AT dm
DROSGENOME1:148328_AT dm
CHICKEN:GGAAFFX.3114.1.S1_AT similar to Sphingomyelin synthase-related 1 (Sterile alpha motif domain containing 8) gga
U133_X3P:HS.276755.0.A1_3P_AT sterile alpha motif domain containing 8 hs
HG-U133_PLUS_2:242062_AT sterile alpha motif domain containing 8 hs
HG-U133B:242062_AT sterile alpha motif domain containing 8 hs
MOE430B:1453730_AT sterile alpha motif domain containing 8 mm
MOUSE430_2:1453730_AT sterile alpha motif domain containing 8 mm
MG-U74CV2:165603_I_AT sterile alpha motif domain containing 8 mm
MG-U74CV2:169146_I_AT sterile alpha motif domain containing 8 mm
MOE430A:1434402_AT sterile alpha motif domain containing 8 mm
MOUSE430A_2:1434402_AT sterile alpha motif domain containing 8 mm
MOUSE430_2:1434402_AT sterile alpha motif domain containing 8 mm
MOE430B:1458408_AT sterile alpha motif domain containing 8 mm
MOUSE430_2:1458408_AT sterile alpha motif domain containing 8 mm
MU19KSUBC:TC41313_AT Sterile alpha motif domain containing 8 (Samd8), mRNA mm
RG-U34B:RC_AI060175_AT sterile alpha motif domain containing 8 (predicted) rn
RAE230B:1393932_AT sterile alpha motif domain containing 8 (predicted) rn
RAT230_2:1393932_AT sterile alpha motif domain containing 8 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAB52647 Hypothetical protein F53B1.2 [Caenorhabditis elegans] ref|NP_508389.1| F53B1.2 [Caenorhabditis elegans] sp|Q20696|SMSR1_CAEEL Sphingomyelin synthase-related 0.0
blast CAE68353 Hypothetical protein CBG14086 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001660 EMBL-EBI Sterile alpha motif SAM 1.9E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508389.1 5 181-203,228-250,262-284,349-371,378-400
F53B1.2.2 5 181-203,228-250,262-284,349-371,378-400
F53B1.2.1 5 181-203,228-250,262-284,349-371,378-400
SNAP00000022989 5 181-203,228-250,262-284,349-371,378-400
GENEFINDER00000023001 5 181-203,228-250,262-284,349-371,378-400
Sequence
Target Sequence 
>C. ELEGANS:181478_S_AT
tctcggtacctgggattcatcttcaatgcgaagccaggccaaacactacaatgcaggaaa
agctacacaaagcattccacatttggagcaaccttggaatgtcacttcacggagttcgta
gttgtggagactacatgttcagtggtcacactacagtgatcacgatgatttctcatttta
tcacagaatatacccctgcagattggactggccttcacacattcacttgggttctcaatt
gttttgccattttcctcattcttgcagcccatgagcactatagtattgatgtttttattg
cattttacatcagctctcgcatgtttttgtattaccacgcctatgcctacaaccacgctg
gtatcactgcaacggattataggatgaggacctggttcccgctcgggtggttctttgaat
atggatcgcaaggaaaagttgaaaatgagttcagtcttccaatcaacatcagaattcctc
gccgtgtgttctttgctaaatcggaggaaccgaagataactccaaagtctgatagtagtc
ggaaaaggtcatccgtggtggctgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCGGTACCTGGGATTCATCTTCA 629 611 854 Antisense
GCAACCTTGGAATGTCACTTCACGG 686 319 941 Antisense
TTCACACATTCACTTGGGTTCTCAA 321 685 1067 Antisense
TCAATTGTTTTGCCATTTTCCTCAT 28 635 1088 Antisense
CATTCTTGCAGCCCATGAGCACTAT 643 215 1110 Antisense
GCTCTCGCATGTTTTTGTATTACCA 411 299 1166 Antisense
CACGCTGGTATCACTGCAACGGATT 475 199 1207 Antisense
ATAGGATGAGGACCTGGTTCCCGCT 544 21 1232 Antisense
GAGTTCAGTCTTCCAATCAACATCA 601 397 1300 Antisense
TCGCCGTGTGTTCTTTGCTAAATCG 108 591 1332 Antisense
GGAAAAGGTCATCCGTGGTGGCTGC 230 527 1394 Antisense
Sequence Source Affymetrix Proprietary Database