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[181475_at]

Description K04A8.8 /REP_DB=WormBase Gene ID /WP=CE11714 /TR=Q94255 /GB=AAC48054.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      6grp_highest_nonorm > 95      6grp_total > 94      4grp_total > 02      6grp_cgc > 88      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 386
1 2 3 4 range
A 17.8
(-2.7)
A 		60.6
(-1.8)
A 		3.1
(-3)
A 		285.9
(3)
P 		283
B 43.1
(-2.8)
A 		40.1
(-2.9)
A 		34.8
(-3)
A 		346.2
(3)
P 		311
C 44.8
(-2.4)
A 		3.3
(-3)
A 		24.7
(-2.7)
A 		389
(3)
P 		386
avr 35.2
(-2.7) 		34.7
(-2.7) 		20.9
(-3) 		340.4
(3) 		320

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1929
1 2 3 4 5 6 range
A 500.6
(-1.2)
P 		306.7
(-2.3)
P 		170.6
(-3)
P 		855.4
(0.7)
P 		1295.9
(3)
P 		932
(1.1)
P 		1125
B 572.6
(-1.2)
P 		366.2
(-2.1)
P 		174
(-3)
P 		512.6
(-1.5)
P 		1520.2
(3)
P 		1119.1
(1.2)
P 		1346
C 712.8
(-1.2)
P 		309.2
(-2.5)
P 		141.3
(-3)
P 		714.1
(-1.2)
P 		2069.8
(3)
P 		941.4
(-0.5)
P 		1929
avr 595.3
(-1.2) 		327.4
(-2.3) 		162
(-3) 		694
(-0.8) 		1628.6
(3) 		997.5
(0.4) 		1467
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 6.5
(-2.2)
A 		13.1
(-1.2)
A 		0.8
(-3)
A 		41.9
(3)
P 		41
B 13.7
(-2.5)
A 		9.9
(-2.8)
A 		8.1
(-3)
A 		73.8
(3)
P 		66
C 14.8
(-1.7)
A 		0.9
(-3)
A 		8.6
(-2.3)
A 		65.6
(3)
P 		65
avr 11.7
(-2.4) 		8
(-2.8) 		5.8
(-3) 		60.4
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 634
1 2 3 4 5 6 range
A 92.3
(-2.1)
P 		109.1
(-1.8)
P 		37.2
(-3)
P 		217
(0)
P 		391
(3)
P 		226.9
(0.2)
P 		354
B 199.2
(-1.4)
P 		121.9
(-2.2)
P 		42.3
(-3)
P 		135.8
(-2)
P 		617.7
(3)
P 		417.3
(0.9)
P 		575
C 264.2
(-0.8)
P 		94.3
(-2.4)
P 		36
(-3)
P 		143.6
(-2)
P 		669.8
(3)
P 		315.1
(-0.4)
P 		634
avr 185.2
(-1.3) 		108.4
(-2.2) 		38.5
(-3) 		165.5
(-1.5) 		559.5
(3) 		319.8
(0.2) 		521
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181475_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11425
Sequence Type Exemplar sequence
Representative Public ID K04A8.8 NCBI
Target Description K04A8.8 /REP_DB=WormBase Gene ID /WP=CE11714 /TR=Q94255 /GB=AAC48054.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072264(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072264 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-20) (spp-20) mRNA, complete cds. 11/11 None
K04A8.8 ENSEMBL cdna:known chromosome:CEL140:V:6559299:6560789:-1 gene:K04A8.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001684 6/11 Cross Hyb Matching Probes None
GENEFINDER00000001694 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6559298-6560789(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-20
Ensembl K04A8.8
Entrez Gene 186973 Entrez gene
SwissProt Q94255 EMBL-EBI
WormBase CE30812 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6629 lipid metabolism inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC48054 Saposin-like protein family protein 20 [Caenorhabditis elegans] ref|NP_504665.2| SaPosin-like Protein family member (spp-20) [Caenorhabditis elegans] 1.0E-122
Protein Domains
Database ID Description E-Value
Pfam IPR007856 EMBL-EBI Saposin-like type B, 1 2.3E-9
Sequence
Target Sequence 
>C. ELEGANS:181475_AT
atttcgttactttcattttggtgcaatttctattcaccattggattttcaacgccgctct
ccaccgaacttccttcttcaaactgtaaaatgttttgtgaactgtgtcaagatggttttt
cattgattcacaaaaacattgaacaatttgaatcagtcactaacgaggaaattgttaaat
ttatcgatcatatttgccagcttgctccaaaactggatatcatcaacgtgagtggtacac
tatccaatataatctgcacaaattgtcaatccgctgttaacgctttcctagcacctatca
agaaagtcgagttgatcaccaaaactgagttgacagattttgtgaacagaaagtgcttac
acgatccacaacttgcatcttttcattttttgtgctcaatggctcttgatcttgcactca
aatttttagacgccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTCGTTACTTTCATTTTGGTGCA 44 15 20 Antisense
GGTGCAATTTCTATTCACCATTGGA 514 501 39 Antisense
TCACCATTGGATTTTCAACGCCGCT 130 625 53 Antisense
ACTTCCTTCTTCAAACTGTAAAATG 38 103 87 Antisense
ATATTTGCCAGCTTGCTCCAAAACT 389 19 209 Antisense
GTACACTATCCAATATAATCTGCAC 204 461 254 Antisense
TGTCAATCCGCTGTTAACGCTTTCC 113 557 283 Antisense
CGCTTTCCTAGCACCTATCAAGAAA 465 255 300 Antisense
CACGATCCACAACTTGCATCTTTTC 689 197 379 Antisense
GCTCAATGGCTCTTGATCTTGCACT 590 301 413 Antisense
TTGCACTCAAATTTTTAGACGCCGT 612 685 431 Antisense
Sequence Source Affymetrix Proprietary Database