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[181474_at]

Description C44E4.5 /REP_DB=WormBase Gene ID /WP=CE08719 /TR=O01809 /GB=AAB54173.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 95      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 93      6grp_total > 86      4grp_total > 01      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 403
1 2 3 4 range
A 321.4
(-1.7)
P 		234.7
(-3)
P 		274.2
(-2.4)
P 		637.7
(3)
P 		403
B 247.1
(-3)
P 		276.1
(-2.5)
P 		246.5
(-3)
P 		609.9
(3)
P 		363
C 272.3
(-2.5)
P 		242.8
(-3)
P 		302.5
(-2)
P 		588.7
(3)
P 		346
avr 280.3
(-2.5) 		251.2
(-3) 		274.4
(-2.6) 		612.1
(3) 		361

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 404
1 2 3 4 5 6 range
A 195.8
(-3)
P 		282.8
(-1.4)
P 		324.8
(-0.7)
P 		419.7
(1.1)
P 		428.8
(1.2)
P 		526
(3)
P 		330
B 309
(-1.8)
P 		245
(-3)
P 		257.5
(-2.8)
P 		400
(-0.2)
P 		373.3
(-0.7)
P 		573.3
(3)
P 		328
C 273.6
(-3)
P 		285.4
(-2.8)
P 		354.6
(-1.5)
P 		439.5
(0)
P 		445.7
(0.2)
P 		600.2
(3)
P 		327
avr 259.5
(-3) 		271.1
(-2.8) 		312.3
(-2) 		419.7
(0.1) 		415.9
(0.1) 		566.5
(3) 		307
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 117
(3)
P 		50.9
(-3)
P 		71.7
(-1.1)
P 		93.4
(0.9)
P 		66
B 78.8
(-1.2)
P 		68.2
(-2.1)
P 		57.1
(-3)
P 		130
(3)
P 		73
C 90.1
(0.4)
P 		69.8
(-3)
P 		105.4
(3)
P 		99.3
(2)
P 		36
avr 95.3
(1.3) 		63
(-3) 		78.1
(-1) 		107.6
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 36.1
(-3)
P 		100.6
(1.1)
P 		70.7
(-0.8)
P 		106.4
(1.5)
P 		129.4
(3)
P 		128.1
(2.9)
P 		93
B 107.5
(-1.2)
P 		81.6
(-2.2)
P 		62.5
(-3)
P 		105.9
(-1.3)
P 		151.7
(0.5)
P 		213.8
(3)
P 		151
C 101.4
(-2.2)
P 		87.1
(-3)
P 		90.3
(-2.8)
P 		88.4
(-2.9)
P 		144.2
(0)
P 		200.9
(3)
P 		114
avr 81.7
(-2.6) 		89.8
(-2.1) 		74.5
(-3) 		100.2
(-1.6) 		141.8
(0.8) 		180.9
(3) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181474_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.535
Sequence Type Exemplar sequence
Representative Public ID C44E4.5 NCBI
Target Description C44E4.5 /REP_DB=WormBase Gene ID /WP=CE08719 /TR=O01809 /GB=AAB54173.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059005(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059005 NCBI Caenorhabditis elegans C44E4.5 (C44E4.5) mRNA, complete cds. 11/11 None
GENEFINDER00000019662 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4605690:4610114:1 11/11 None
SNAP00000019657 ENSEMBL cdna:SNAP chromosome:CEL140:I:4607394:4629937:1 11/11 None
C44E4.5.2 ENSEMBL cdna:known chromosome:CEL140:I:4605654:4610203:1 gene:C44E4.5 11/11 None
C44E4.5.1 ENSEMBL cdna:known chromosome:CEL140:I:4605656:4610203:1 gene:C44E4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4605689-4610114(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C44E4.5
Entrez Gene 172067 Entrez gene
SwissProt O01809 EMBL-EBI
WormBase CE08719 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB54173 Hypothetical protein C44E4.5 [Caenorhabditis elegans] ref|NP_491406.1| C44E4.5 [Caenorhabditis elegans] 0.0
blast CAE74513 Hypothetical protein CBG22267 [Caenorhabditis briggsae] 7.0E-93
blast CAE74513 Hypothetical protein CBG22267 [Caenorhabditis briggsae] 2.0E-90
blast CAE74513 Hypothetical protein CBG22267 [Caenorhabditis briggsae] 5.0E-81
Sequence
Target Sequence 
>C. ELEGANS:181474_AT
gtcaggttctttttacatcacgtcaatcctccacctctcctgctctcatttgtcttcgtc
acgacgttgacggtcttctgtggtctttcgacgaatctgcacccagccacgtggcaactc
ttcaagcttttggatacgttcaagcttcgaagacaagccgtctctggtccggatgctcac
caaacaagtctttggcgtgtattgtagaaggaaataatcgagttctgctttactcgcaaa
aagttgaagtttctggaagtctgagcaatcgaaaaacatcgcaaacagtgagccacgttt
caaaacaacacttgctccgcgtcgagtgctctgatccgattcgtggagtccagcttaccg
atac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAGGTTCTTTTTACATCACGTCA 694 465 1691 Antisense
CTCATTTGTCTTCGTCACGACGTTG 298 229 1735 Antisense
ACGACGTTGACGGTCTTCTGTGGTC 79 93 1751 Antisense
GTGGTCTTTCGACGAATCTGCACCC 589 489 1770 Antisense
ACGTGGCAACTCTTCAAGCTTTTGG 55 95 1799 Antisense
TTCGAAGACAAGCCGTCTCTGGTCC 157 693 1836 Antisense
CTGGTCCGGATGCTCACCAAACAAG 610 239 1854 Antisense
AACATCGCAAACAGTGAGCCACGTT 63 135 1965 Antisense
GTTTCAAAACAACACTTGCTCCGCG 680 445 1987 Antisense
AGTGCTCTGATCCGATTCGTGGAGT 38 61 2015 Antisense
TTCGTGGAGTCCAGCTTACCGATAC 343 691 2030 Antisense
Sequence Source Affymetrix Proprietary Database