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[181463_at]

Description F45F2.5 /REP_DB=WormBase Gene ID /WP=CE10526 /TR=Q22978 /GB=AAC48028.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 92      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 36.9
(0.4)
A 		58.4
(3)
A 		8.8
(-3)
A 		33.1
(-0.1)
A 		50
B 44.8
(2.8)
A 		15.5
(-3)
A 		20.8
(-2)
A 		45.9
(3)
P 		30
C 56.7
(3)
A 		31.6
(0.1)
A 		3.9
(-3)
A 		52.7
(2.5)
A 		53
avr 46.1
(3) 		35.2
(1.1) 		11.2
(-3) 		43.9
(2.6) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 17.1
(-2.2)
A 		25.6
(-1.4)
A 		45.2
(0.5)
A 		70.1
(3)
A 		57.5
(1.8)
A 		9.4
(-3)
A 		61
B 36.8
(-1.1)
A 		48.8
(0.2)
A 		29.6
(-1.9)
A 		65.3
(2)
A 		74.1
(3)
A 		19.1
(-3)
A 		55
C 47.2
(0.5)
A 		20.7
(-2)
A 		32.9
(-0.9)
A 		10.5
(-3)
A 		74.2
(3)
A 		35.2
(-0.7)
A 		64
avr 33.7
(-1.4) 		31.7
(-1.7) 		35.9
(-1.1) 		48.6
(0.5) 		68.6
(3) 		21.2
(-3) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 13.4
(3)
A 		12.7
(2.6)
A 		2.3
(-3)
A 		4.8
(-1.6)
A 		11
B 14.3
(3)
A 		3.8
(-3)
A 		4.8
(-2.4)
A 		9.8
(0.4)
P 		11
C 18.8
(3)
A 		9.1
(-0.3)
A 		1.4
(-3)
A 		8.9
(-0.4)
A 		17
avr 15.5
(3) 		8.5
(-0.3) 		2.8
(-3) 		7.8
(-0.6) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 3.2
(-2.7)
A 		9.1
(-0.4)
A 		9.8
(-0.1)
A 		17.8
(3)
A 		17.3
(2.8)
A 		2.3
(-3)
A 		16
B 12.8
(-1.5)
A 		16.2
(-0.6)
A 		7.2
(-3)
A 		17.3
(-0.3)
A 		30.1
(3)
A 		7.1
(-3)
A 		23
C 17.5
(1.2)
A 		6.3
(-1.8)
A 		8.4
(-1.3)
A 		2.1
(-3)
A 		24
(3)
A 		11.8
(-0.3)
A 		22
avr 11.2
(-1.5) 		10.5
(-1.8) 		8.5
(-2.5) 		12.4
(-1.1) 		23.8
(3) 		7.1
(-3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181463_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11658
Sequence Type Exemplar sequence
Representative Public ID F45F2.5 NCBI
Target Description F45F2.5 /REP_DB=WormBase Gene ID /WP=CE10526 /TR=Q22978 /GB=AAC48028.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072795(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072795 NCBI Caenorhabditis elegans F45F2.5 (F45F2.5) mRNA, complete cds. 11/11 None
SNAP00000030770 ENSEMBL cdna:SNAP chromosome:CEL140:V:8533055:8535054:1 11/11 None
GENEFINDER00000030782 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8533055:8535054:1 11/11 None
F45F2.5 ENSEMBL cdna:known chromosome:CEL140:V:8533028:8535109:1 gene:F45F2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8533028-8535055(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F45F2.5
Entrez Gene 185806 Entrez gene
SwissProt Q22978 EMBL-EBI
WormBase CE10526 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48028 Hypothetical protein F45F2.5 [Caenorhabditis elegans] ref|NP_505196.1| F45F2.5 [Caenorhabditis elegans] 0.0
blast CAE58380 Hypothetical protein CBG01509 [Caenorhabditis briggsae] 0.0
blast NP_504136 D1065.1 [Caenorhabditis elegans] gb|AAG24020.1| Hypothetical protein D1065.1 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.0E-126
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.5E-33
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.0E-126
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505196.1 11 34-56,71-93,132-154,159-181,194-216,272-294,318-340,355-377,396-418,428-450,470-487
F45F2.5 11 34-56,71-93,132-154,159-181,194-216,272-294,318-340,355-377,396-418,428-450,470-487
SNAP00000030770 9 25-47,62-84,123-145,214-236,260-282,297-319,338-360,370-392,412-429
GENEFINDER00000030782 10 25-47,60-82,123-145,152-171,263-285,309-331,346-368,387-409,419-441,461-478
Sequence
Target Sequence 
>C. ELEGANS:181463_AT
gatgtatttctgttcgttgttgcacttcttgcttgtattctagcattctttcaaatgcga
aaactacaatataggcttcatgctcacggagaagtcattgatgagattctgttaattgtg
ggattagctggagaatgtgtgtactcatgtgctggaatggatttatatatcaatgacaag
atgattaaagaacctgcttcatacattacaattgtatcattcacattgagaatcaccgaa
gtcttggttcaaacaatttttatccttattgctactagaatgagatgctacacccagacc
aattgttacactgagccaggaaaacaagtgataacatttcttctcgtatgtaatatcaat
ctatttatctatcacacattcgaaactactgaattgaactttggatttcccatcaaactg
cctgatagctactccgctatgcttaatatagcttcgccaattgtagttttctatcggttt
cacagttcagcatgtttggcggagatttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGTATTTCTGTTCGTTGTTGCAC 33 411 1093 Antisense
GTTGTTGCACTTCTTGCTTGTATTC 461 437 1108 Antisense
AATATAGGCTTCATGCTCACGGAGA 388 173 1160 Antisense
GAATGTGTGTACTCATGTGCTGGAA 314 333 1225 Antisense
AGAACCTGCTTCATACATTACAATT 638 73 1281 Antisense
GCTACACCCAGACCAATTGTTACAC 246 303 1379 Antisense
ATTTCCCATCAAACTGCCTGATAGC 522 17 1497 Antisense
CCTGATAGCTACTCCGCTATGCTTA 75 265 1513 Antisense
ATAGCTTCGCCAATTGTAGTTTTCT 9 23 1540 Antisense
GTTTTCTATCGGTTTCACAGTTCAG 166 449 1558 Antisense
GTTCAGCATGTTTGGCGGAGATTTG 217 445 1577 Antisense
Sequence Source Affymetrix Proprietary Database