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[181445_s_at]

Description F58D5.4A /REP_DB=WormBase Gene ID /WP=CE25020 /TR=Q9NLD0 /GB=CAB70239.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_high_8_8 > 07      6grp_highest_nonorm > 90      6grp_total > 68      4grp_total > 04      6grp_highest_sub > Da      6grp_cgc > 38      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 259
1 2 3 4 range
A 109.7
(-1.5)
P 		60.9
(-2.6)
P 		45.3
(-3)
A 		304.7
(3)
P 		259
B 107.9
(-1.2)
P 		59.4
(-3)
A 		101.7
(-1.4)
P 		216.9
(3)
P 		158
C 100.8
(-1.6)
P 		64.3
(-3)
A 		70.5
(-2.8)
M 		219.7
(3)
P 		155
avr 106.1
(-1.6) 		61.5
(-3) 		72.5
(-2.6) 		247.1
(3) 		186

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 246
1 2 3 4 5 6 range
A 138
(-0.4)
P 		63.9
(-3)
P 		98.5
(-1.8)
P 		77.5
(-2.5)
P 		131.4
(-0.6)
P 		236.1
(3)
P 		172
B 124
(-2.3)
P 		97.8
(-3)
P 		126.5
(-2.2)
P 		113.9
(-2.5)
P 		107.6
(-2.7)
P 		309.7
(3)
P 		212
C 75.4
(-2.7)
P 		88.4
(-2.4)
P 		64.4
(-3)
P 		81
(-2.6)
P 		118.9
(-1.7)
P 		308.4
(3)
P 		244
avr 112.5
(-2.1) 		83.4
(-3) 		96.5
(-2.6) 		90.8
(-2.8) 		119.3
(-1.9) 		284.7
(3) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 39.9
(2.1)
P 		13.2
(-2.7)
P 		11.8
(-3)
A 		44.6
(3)
P 		33
B 34.4
(0.8)
P 		14.7
(-3)
A 		23.6
(-1.3)
P 		46.2
(3)
P 		32
C 33.4
(1.8)
P 		18.5
(-3)
A 		24.6
(-1)
M 		37
(3)
P 		19
avr 35.9
(1.5) 		15.5
(-3) 		20
(-2) 		42.6
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 25.4
(-2.1)
P 		22.7
(-2.5)
P 		21.4
(-2.7)
P 		19.6
(-3)
P 		39.7
(0.2)
P 		57.5
(3)
P 		38
B 43.1
(-2.1)
P 		32.6
(-2.8)
P 		30.7
(-3)
P 		30.2
(-3)
P 		43.7
(-2.1)
P 		115.5
(3)
P 		85
C 27.9
(-2.2)
P 		27
(-2.3)
P 		16.4
(-3)
P 		16.3
(-3)
P 		38.5
(-1.5)
P 		103.3
(3)
P 		87
avr 32.1
(-2.1) 		27.4
(-2.5) 		22.8
(-2.9) 		22
(-3) 		40.6
(-1.4) 		92.1
(3) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181445_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.845
Sequence Type Exemplar sequence
Representative Public ID F58D5.4A NCBI
Target Description F58D5.4A /REP_DB=WormBase Gene ID /WP=CE25020 /TR=Q9NLD0 /GB=CAB70239.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026347(11), NM_001026348(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026347 NCBI Caenorhabditis elegans Kinase Suppressor of activated Ras family member (ksr-2) (ksr-2) mRNA, complete cds. 11/11 None
NM_001026348 NCBI Caenorhabditis elegans Kinase Suppressor of activated Ras family member (ksr-2) (ksr-2) mRNA, complete cds. 11/11 None
SNAP00000034699 ENSEMBL cdna:SNAP chromosome:CEL140:I:12053358:12065408:-1 11/11 None
GENEFINDER00000034710 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12053437:12060510:-1 11/11 None
F58D5.4b ENSEMBL cdna:known chromosome:CEL140:I:12055834:12067920:-1 gene:F58D5.4 11/11 A 
F58D5.4a ENSEMBL cdna:known chromosome:CEL140:I:12055834:12067920:-1 gene:F58D5.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12055937-12067835(-) 100.0 100.0
Public Domain and Genome References
Ensembl F58D5.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70239 Hypothetical protein F58D5.4a [Caenorhabditis elegans] ref|NP_001021518.1| Kinase Suppressor of activated Ras family member (ksr-2) [Caenorhabditis elegans] gb|AAL79358.1| KSR-2a [Caenorhabditis elegans] 0.0
blast CAB70240 Hypothetical protein F58D5.4b [Caenorhabditis elegans] ref|NP_001021519.1| Kinase Suppressor of activated Ras family member (ksr-2) [Caenorhabditis elegans] gb|AAL79359.1| KSR-2b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 4.80000019073486
Pfam IPR000719 EMBL-EBI Protein kinase 2.8E-24
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-5
Pfam IPR000719 EMBL-EBI Protein kinase 2.8E-24
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-5
Pfam IPR000719 EMBL-EBI Protein kinase 2.8E-24
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-5
Pfam IPR000719 EMBL-EBI Protein kinase 5.9E-19
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000034710 2 319-336,423-442
Sequence
Target Sequence 
>C. ELEGANS:181445_S_AT
ggacaatccgaatcgagtcgttgttacggattttgcgttgatgaagcttgagcggctgga
gaatccccgcagaaattgtacacttttaatcccaaatcactggattgactatctttcgcc
ggaaattgctggaaatttgatgattgattggaggggtgatgtgttgtttcaacatgaact
accgttttcacaagaatctgatgtttattcgtttggtacaattttcttcgaactcctgct
ccgccgtatgccaacgggctgtgactcttgggatcagaagctctacgcgaaaatgtgcgg
ccaaaaagcggcacttcaacggctcgacgcccagctgcaaaaaatcgacggaaaattgca
cgaactgctgctggaatgctggtcgagccaaccggagaagcggccgagtttccaacaaat
tg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACAATCCGAATCGAGTCGTTGTT 458 525 1221 Antisense
AGCTTGAGCGGCTGGAGAATCCCCG 310 77 1265 Antisense
GAATCCCCGCAGAAATTGTACACTT 289 329 1281 Antisense
GTACACTTTTAATCCCAAATCACTG 131 461 1298 Antisense
GACTATCTTTCGCCGGAAATTGCTG 332 371 1327 Antisense
TCCGCCGTATGCCAACGGGCTGTGA 30 595 1460 Antisense
GGGCTGTGACTCTTGGGATCAGAAG 311 493 1476 Antisense
AAAAAGCGGCACTTCAACGGCTCGA 515 129 1523 Antisense
GAACTGCTGCTGGAATGCTGGTCGA 520 347 1582 Antisense
TCGAGCCAACCGGAGAAGCGGCCGA 508 599 1603 Antisense
AAGCGGCCGAGTTTCCAACAAATTG 572 149 1618 Antisense
Sequence Source Affymetrix Proprietary Database