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[181424_at]

Description F40G12.4 /REP_DB=WormBase Gene ID /WP=CE10182 /TR=Q20250 /GB=CAB01187.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 02      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 690
1 2 3 4 range
A 38.5
(-2.8)
A 		30.9
(-2.9)
A 		15.2
(-3)
A 		647
(3)
P 		632
B 18.5
(-2.9)
A 		33.5
(-2.7)
A 		4.1
(-3)
A 		581.6
(3)
P 		578
C 3
(-3)
A 		29
(-2.8)
A 		40.9
(-2.7)
A 		693.2
(3)
P 		690
avr 20
(-3) 		31.1
(-2.9) 		20.1
(-3) 		640.6
(3) 		621

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 330
1 2 3 4 5 6 range
A 29.3
(-2.5)
A 		6.4
(-3)
A 		42.8
(-2.2)
A 		41.5
(-2.3)
A 		106
(-0.9)
A 		293.1
(3)
P 		287
B 18.6
(-2.8)
A 		6.7
(-3)
A 		23.8
(-2.7)
A 		5
(-3)
A 		146
(-0.4)
P 		332
(3)
P 		327
C 2.4
(-3)
A 		46.6
(-1.9)
A 		8.3
(-2.9)
A 		9.4
(-2.8)
A 		79.3
(-1.1)
A 		240.4
(3)
P 		238
avr 16.8
(-3) 		19.9
(-2.9) 		25
(-2.8) 		18.6
(-3) 		110.4
(-0.9) 		288.5
(3) 		272
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 123
1 2 3 4 range
A 14
(-2.3)
A 		6.7
(-2.8)
A 		4
(-3)
A 		94.7
(3)
P 		91
B 5.9
(-2.8)
A 		8.3
(-2.6)
A 		0.9
(-3)
A 		123.9
(3)
P 		123
C 1
(-3)
A 		8.3
(-2.6)
A 		14.3
(-2.3)
A 		116.9
(3)
P 		116
avr 7
(-3) 		7.8
(-2.9) 		6.4
(-3) 		111.8
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 5.4
(-2.7)
A 		2.3
(-3)
A 		9.3
(-2.4)
A 		10.5
(-2.3)
A 		32
(-0.4)
A 		71.4
(3)
P 		69
B 6.5
(-2.7)
A 		2.2
(-3)
A 		5.8
(-2.8)
A 		1.3
(-3)
A 		59.3
(-0.2)
P 		123.8
(3)
P 		123
C 0.9
(-3)
A 		14.2
(-2)
A 		2.1
(-2.9)
A 		1.9
(-2.9)
A 		25.7
(-1.1)
A 		80.5
(3)
P 		80
avr 4.3
(-3) 		6.2
(-2.9) 		5.7
(-2.9) 		4.6
(-3) 		39
(-0.6) 		91.9
(3) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181424_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12522
Sequence Type Exemplar sequence
Representative Public ID F40G12.4 NCBI
Target Description F40G12.4 /REP_DB=WormBase Gene ID /WP=CE10182 /TR=Q20250 /GB=CAB01187.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074130
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000026757 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14269961:14271227:-1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_074130 8/11 Cross Hyb Matching Probes None
GENEFINDER00000006126 2/11 Cross Hyb Matching Probes None
SNAP00000026729 8/11 Cross Hyb Matching Probes None
GENEFINDER00000029741 2/11 Cross Hyb Matching Probes None
F40G12.4 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14270189-14271227(-) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01187 Hypothetical protein F40G12.4 [Caenorhabditis elegans] ref|NP_506531.2| F40G12.4 [Caenorhabditis elegans] 1.0E-143
blast CAE60978 Hypothetical protein CBG04712 [Caenorhabditis briggsae] 1.0E-100
blast CAB01183 Hypothetical protein F40G12.5 [Caenorhabditis elegans] ref|NP_506532.1| F40G12.5 [Caenorhabditis elegans] 1.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR004920 EMBL-EBI Protein of unknown function DUF263 1.8E-54
Sequence
Target Sequence 
>C. ELEGANS:181424_AT
gagctcgtggatgctctttcttttattccagcacatggaggatgctcggatacatattat
agattaaatgctcaaaggtgtgacgcgcagaagacaatgatggagtgggaaattgagaag
caattagaaagattgccaaagttcaaaaattcaacgcaagtaatcgccatgtgcagggaa
ataaaggaatgtatggaagacctttgcttcaccgaagactctcagtacgaaatcgaattt
agtcttgctgttccagaacttacagtgtctcatttcacagtctgtataaacacgatcgat
aaggaattacctgacctttcaaagtacagatgtctcgaaaatcgaagtttcttcaagaaa
accccagaatacatgtgtgaaagatatcgaaatagcaacagggaatgtttgagagccgtt
acaaaagggtactgtggacgggatgttgtgaaaccagtggaaaagtatctgaacgaattt
gtaaaattgaaatggccgcgcgcacgcagccccgatacgcaaaaattaagctgttggcga
atctcaactcgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCTCGTGGATGCTCTTTCTTTTA 685 385 304 Antisense
CTCTTTCTTTTATTCCAGCACATGG 198 231 317 Antisense
ATTCAACGCAAGTAATCGCCATGTG 269 11 452 Antisense
AGACCTTTGCTTCACCGAAGACTCT 311 69 501 Antisense
GAATTTAGTCTTGCTGTTCCAGAAC 198 325 538 Antisense
GTTCCAGAACTTACAGTGTCTCATT 353 441 553 Antisense
GTGTCTCATTTCACAGTCTGTATAA 316 487 568 Antisense
GAATTACCTGACCTTTCAAAGTACA 97 323 607 Antisense
GTAAAATTGAAATGGCCGCGCGCAC 263 461 784 Antisense
ACGCAGCCCCGATACGCAAAAATTA 634 81 807 Antisense
AGCTGTTGGCGAATCTCAACTCGAG 500 81 832 Antisense
Sequence Source Affymetrix Proprietary Database