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[181409_at]

Description F41E6.8 /REP_DB=WormBase Gene ID /WP=CE10258 /TR=O16456 /GB=AAB65958.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 33      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 2.9
(-3)
A 		30.6
(1.2)
A 		31.5
(1.3)
A 		42.7
(3)
A 		40
B 6
(-2.5)
A 		14.6
(3)
A 		11
(0.7)
A 		5.3
(-3)
A 		9
C 5.7
(-3)
A 		15
(3)
A 		15
(3)
A 		9.3
(-0.7)
A 		9
avr 4.9
(-3) 		20.1
(3) 		19.2
(2.6) 		19.1
(2.6) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 34.4
(0.4)
A 		12
(-3)
A 		26.8
(-0.8)
A 		16.9
(-2.3)
A 		51.6
(3)
A 		26
(-0.9)
A 		40
B 8.3
(-3)
A 		8.3
(-3)
A 		8.9
(-2.9)
A 		8.8
(-2.9)
A 		13.3
(-2.2)
A 		46.1
(3)
A 		38
C 12.6
(-2.1)
A 		10.5
(-2.4)
A 		5.8
(-3)
A 		16.4
(-1.7)
A 		53.5
(3)
A 		37.5
(1)
A 		48
avr 18.4
(-1.3) 		10.3
(-3) 		13.8
(-2.3) 		14
(-2.2) 		39.5
(3) 		36.5
(2.4) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.1
(-3)
A 		6.6
(1.6)
A 		8.2
(3)
A 		6.2
(1.3)
A 		7
B 1.9
(-1.1)
A 		3.6
(3)
A 		2.5
(0.4)
A 		1.1
(-3)
A 		3
C 1.9
(-2.5)
A 		4.3
(1.5)
A 		5.2
(3)
A 		1.6
(-3)
A 		4
avr 1.6
(-3) 		4.8
(2.2) 		5.3
(3) 		3
(-0.7) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 6.3
(-1.9)
A 		4.3
(-3)
A 		5.8
(-2.2)
A 		4.3
(-3)
A 		15.6
(3)
A 		6.3
(-1.9)
A 		11
B 2.9
(-2.7)
A 		2.8
(-2.8)
A 		2.2
(-3)
A 		2.3
(-3)
A 		5.4
(-1.7)
A 		17.2
(3)
A 		15
C 4.7
(-1.8)
A 		3.2
(-2.4)
A 		1.5
(-3)
A 		3.3
(-2.3)
A 		17.3
(3)
A 		12.5
(1.2)
A 		16
avr 4.6
(-2.1) 		3.4
(-2.9) 		3.2
(-3) 		3.3
(-2.9) 		12.8
(3) 		12
(2.5) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181409_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12182
Sequence Type Exemplar sequence
Representative Public ID F41E6.8 NCBI
Target Description F41E6.8 /REP_DB=WormBase Gene ID /WP=CE10258 /TR=O16456 /GB=AAB65958.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072816(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072816 NCBI Caenorhabditis elegans F41E6.8 (F41E6.8) mRNA, complete cds. 11/11 None
GENEFINDER00000009436 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8600439:8604000:-1 11/11 None
F41E6.8 ENSEMBL cdna:known chromosome:CEL140:V:8602172:8604000:-1 gene:F41E6.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009421 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8602172-8604001(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F41E6.8
Entrez Gene 185619 Entrez gene
SwissProt O16456 EMBL-EBI
WormBase CE10258 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65958 Hypothetical protein F41E6.8 [Caenorhabditis elegans] ref|NP_505217.1| F41E6.8 [Caenorhabditis elegans] 1.0E-125
blast AAB65958 Hypothetical protein F41E6.8 [Caenorhabditis elegans] ref|NP_505217.1| F41E6.8 [Caenorhabditis elegans] 5.0E-91
blast CAE58360 Hypothetical protein CBG01482 [Caenorhabditis briggsae] 4.0E-68
blast NP_503623 Y5H2A.1 [Caenorhabditis elegans] gb|AAF59625.1| Hypothetical protein Y5H2A.1 [Caenorhabditis elegans] 3.0E-66
blast NP_503623 Y5H2A.1 [Caenorhabditis elegans] gb|AAF59625.1| Hypothetical protein Y5H2A.1 [Caenorhabditis elegans] 3.0E-63
blast CAE68882 Hypothetical protein CBG14848 [Caenorhabditis briggsae] 6.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 2.3E-54
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 1.8E-15
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 2.3E-54
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 1.8E-15
Sequence
Target Sequence 
>C. ELEGANS:181409_AT
tgcagacgaacgtgtcttccgacacaacaagttgactcttgcacagtttgtccaaaaaca
tatgacatgaattgccaaggatttgggattccaaatttaattaattggtgtcctactgct
gcagaagttggagtcacttacgttgttggtctactctccatactgcccttgcttccagcc
gatacttgctcaacaacagttgtttgtccattaggaacaacagtattttacaatgttctc
ggtacggaactgcctggaccgtcaccgattattgcgtggtgcagacaatctggtgctggt
gctggaacctggaacacaggaattcctccactgtctgcagaattaagttctctagtttgt
cgacctattgttgcggcagatattttttctttctacaaacagggctcccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCAGACGAACGTGTCTTCCGACAC 477 577 55 Antisense
GTTGACTCTTGCACAGTTTGTCCAA 215 439 85 Antisense
TTAATTGGTGTCCTACTGCTGCAGA 138 683 155 Antisense
GTCACTTACGTTGTTGGTCTACTCT 419 463 187 Antisense
CTTCCAGCCGATACTTGCTCAACAA 596 219 226 Antisense
TCTCGGTACGGAACTGCCTGGACCG 155 607 291 Antisense
GGACCGTCACCGATTATTGCGTGGT 39 523 310 Antisense
GACAATCTGGTGCTGGTGCTGGAAC 192 365 338 Antisense
GAACACAGGAATTCCTCCACTGTCT 602 351 366 Antisense
GTTTGTCGACCTATTGTTGCGGCAG 471 449 409 Antisense
TTCTTTCTACAAACAGGGCTCCCAA 622 687 441 Antisense
Sequence Source Affymetrix Proprietary Database