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[181403_at]

Description K04A8.1 /REP_DB=WormBase Gene ID /WP=CE21019 /TR=Q94259 /GB=AAC48047.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 53      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 63      6grp_total > 62      4grp_total > 56      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 579
1 2 3 4 range
A 403.3
(-0.4)
P 		567.6
(1.6)
P 		686.7
(3)
P 		188.3
(-3)
P 		498
B 365.9
(-1.6)
P 		580.8
(0.9)
P 		766.9
(3)
P 		238.2
(-3)
P 		529
C 427.9
(-1.5)
P 		491.7
(-0.5)
P 		706.9
(3)
P 		336.5
(-3)
P 		370
avr 399
(-1.1) 		546.7
(0.8) 		720.2
(3) 		254.3
(-3) 		466

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 589
1 2 3 4 5 6 range
A 398.7
(-3)
P 		775
(0.8)
P 		987.2
(3)
P 		819.1
(1.3)
P 		500.7
(-2)
P 		593.4
(-1)
P 		589
B 534.9
(-3)
P 		743.3
(0.7)
P 		872.9
(3)
P 		788
(1.5)
P 		559.2
(-2.6)
P 		632.8
(-1.3)
P 		338
C 409.5
(-3)
P 		789.6
(1)
P 		764.6
(0.7)
P 		986.1
(3)
P 		466.4
(-2.4)
P 		653.4
(-0.5)
P 		577
avr 447.7
(-3) 		769.3
(1.5) 		874.9
(3) 		864.4
(2.9) 		508.8
(-2.1) 		626.5
(-0.5) 		427
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 146.8
(1.7)
P 		123.2
(0.8)
P 		179.5
(3)
P 		27.6
(-3)
P 		152
B 116.7
(0.1)
P 		143.4
(1.4)
P 		177.7
(3)
P 		50.8
(-3)
P 		127
C 141.7
(-0.3)
P 		141.4
(-0.3)
P 		246.4
(3)
P 		56.7
(-3)
P 		190
avr 135.1
(0.5) 		136
(0.5) 		201.2
(3) 		45
(-3) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 202
1 2 3 4 5 6 range
A 73.5
(-3)
P 		275.6
(3)
P 		215
(1.2)
P 		207.7
(1)
P 		151.1
(-0.7)
P 		144.5
(-0.9)
P 		202
B 186.1
(-3)
P 		247.4
(3)
P 		212
(-0.5)
P 		208.7
(-0.8)
P 		227.2
(1)
P 		236
(1.9)
P 		61
C 151.8
(-2.9)
P 		240.9
(3)
P 		194.6
(-0.1)
P 		198.3
(0.2)
P 		150.9
(-3)
P 		218.7
(1.5)
P 		90
avr 137.1
(-3) 		254.6
(3) 		207.2
(0.6) 		204.9
(0.5) 		176.4
(-1) 		199.7
(0.2) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181403_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12162
Sequence Type Exemplar sequence
Representative Public ID K04A8.1 NCBI
Target Description K04A8.1 /REP_DB=WormBase Gene ID /WP=CE21019 /TR=Q94259 /GB=AAC48047.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072268(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072268 NCBI Caenorhabditis elegans K04A8.1 (K04A8.1) mRNA, complete cds. 11/11 None
GENEFINDER00000001697 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6569170:6571197:1 11/11 None
K04A8.1.1 ENSEMBL cdna:known chromosome:CEL140:V:6569152:6571278:1 gene:K04A8.1 11/11 None
K04A8.1.2 ENSEMBL cdna:known chromosome:CEL140:V:6569153:6571197:1 gene:K04A8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000001689 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6569169-6571197(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K04A8.1
Entrez Gene 179047 Entrez gene
SwissProt Q94259 EMBL-EBI
WormBase CE21019 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48047 Hypothetical protein K04A8.1 [Caenorhabditis elegans] ref|NP_504669.1| K04A8.1 [Caenorhabditis elegans] 0.0
blast AAB70361 Hypothetical protein K06H6.1 [Caenorhabditis elegans] ref|NP_503255.1| K06H6.1 [Caenorhabditis elegans] 1.0E-139
blast CAE71910 Hypothetical protein CBG18972 [Caenorhabditis briggsae] 1.0E-137
Protein Domains
Database ID Description E-Value
Pfam IPR004951 EMBL-EBI Caenorhabditis protein of unknown function DUF268 1.0E-126
Pfam IPR004951 EMBL-EBI Caenorhabditis protein of unknown function DUF268 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504669.1 1 13-32
K04A8.1.1 1 13-32
K04A8.1.2 1 13-32
GENEFINDER00000001697 1 13-32
Sequence
Target Sequence 
>C. ELEGANS:181403_AT
gtcaatatacgacgctatgcatgcctaccggcttgatggaaagagtggtgttgttattgg
aagtatgcaaccatgggttgaagtttcagctcttcaaagtggtgcttccaaagttctcac
tgttgaatataacaaattgactattcaggaagaattcagggatagaatgtcttccattct
tcctatcgattttgtgaaaaattggcgggactatgctggaacgtttgattttgctgcatc
cttctcttctatagagcattctggattaggaagatacggtgatccagttgatccgcttgg
tgacttgagagaaatgctaaaaatcaaatgtgtcttgaaaccaggaggactgttattcat
cggttttccattgggaacggattctattcaatacaacgcgcatagaatttatggatcaat
tcgacttgctatgatgatgtacggtttcgaatggcttgacacattctccggagacaagga
agaagcaaatgatcttacttctgatcgacttcattcaaaacccatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAATATACGACGCTATGCATGCC 427 463 576 Antisense
TATGCATGCCTACCGGCTTGATGGA 275 655 591 Antisense
AGTGGTGCTTCCAAAGTTCTCACTG 362 59 673 Antisense
TCCATTCTTCCTATCGATTTTGTGA 625 591 748 Antisense
TGGCGGGACTATGCTGGAACGTTTG 598 539 778 Antisense
GAACGTTTGATTTTGCTGCATCCTT 620 347 794 Antisense
CGGTGATCCAGTTGATCCGCTTGGT 303 243 852 Antisense
GGAGGACTGTTATTCATCGGTTTTC 31 517 919 Antisense
GAATGGCTTGACACATTCTCCGGAG 498 333 1024 Antisense
ATCTTACTTCTGATCGACTTCATTC 479 31 1067 Antisense
GATCGACTTCATTCAAAACCCATAT 401 417 1078 Antisense
Sequence Source Affymetrix Proprietary Database