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[181396_at]

Description T20F7.5 /REP_DB=WormBase Gene ID /WP=CE13838 /TR=O02166 /GB=AAK18980.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 25      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 64.4
(-1.3)
A 		70.6
(2.4)
A 		71.6
(3)
A 		61.5
(-3)
A 		10
B 69.5
(-1.9)
A 		60.3
(-2.5)
A 		53.5
(-3)
A 		143.6
(3)
A 		90
C 7.4
(-3)
A 		90.6
(3)
A 		60.8
(0.9)
A 		84.6
(2.6)
A 		83
avr 47.1
(-3) 		73.8
(0.2) 		62
(-1.2) 		96.6
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 70.1
(0.5)
A 		62.3
(-0.1)
A 		18.1
(-3)
A 		90.4
(1.8)
A 		81.9
(1.2)
A 		108.4
(3)
A 		90
B 27
(-3)
A 		55
(-0.2)
A 		59.3
(0.2)
A 		87.4
(3)
A 		80.9
(2.4)
A 		55.6
(-0.2)
A 		60
C 47.9
(-2)
A 		70.4
(-0.1)
A 		35.4
(-3)
A 		65.9
(-0.5)
A 		107.6
(3)
A 		68.6
(-0.2)
A 		72
avr 48.3
(-1.8) 		62.6
(-0.1) 		37.6
(-3) 		81.2
(2) 		90.1
(3) 		77.5
(1.6) 		53
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 23.5
(3)
A 		15.3
(-0.4)
A 		18.7
(1)
A 		9
(-3)
A 		15
B 22.1
(0.2)
A 		14.9
(-2.2)
A 		12.4
(-3)
A 		30.6
(3)
A 		18
C 2.4
(-3)
A 		26
(3)
A 		21.2
(1.8)
A 		14.3
(0)
A 		24
avr 16
(-3) 		18.7
(3) 		17.4
(0.1) 		18
(1.4) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 12.9
(-0.6)
A 		22.1
(1.9)
A 		3.9
(-3)
A 		22.9
(2.1)
A 		24.7
(2.5)
A 		26.4
(3)
A 		23
B 9.4
(-3)
A 		18.3
(-0.7)
A 		14.4
(-1.7)
A 		23.2
(0.5)
A 		32.9
(3)
A 		20.7
(-0.1)
A 		24
C 17.8
(-1)
A 		21.5
(-0.1)
A 		9
(-3)
A 		13.3
(-2)
A 		34.8
(3)
A 		22.9
(0.2)
A 		26
avr 13.4
(-1.8) 		20.6
(0.2) 		9.1
(-3) 		19.8
(-0) 		30.8
(3) 		23.3
(0.9) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181396_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17641
Sequence Type Exemplar sequence
Representative Public ID T20F7.5 NCBI
Target Description T20F7.5 /REP_DB=WormBase Gene ID /WP=CE13838 /TR=O02166 /GB=AAK18980.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078322(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078322 NCBI Caenorhabditis elegans T20F7.5 (T20F7.5) mRNA, complete cds. 11/11 A 
SNAP00000037346 ENSEMBL cdna:SNAP chromosome:CEL140:X:16850191:16855407:-1 11/11 None
GENEFINDER00000037355 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16852292:16856036:-1 11/11 None
T20F7.5 ENSEMBL cdna:known chromosome:CEL140:X:16845289:16855407:-1 gene:T20F7.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16852303-16855407(-) 99.1 100.0
Public Domain and Genome References
Gene Title
Ensembl T20F7.5
Entrez Gene 181733 Entrez gene
SwissProt O02165 EMBL-EBI
WormBase CE30187 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18983 Hypothetical protein T20F7.5 [Caenorhabditis elegans] ref|NP_510723.2| T20F7.5 [Caenorhabditis elegans] 0.0
blast T25897 hypothetical protein T20F7.5 - Caenorhabditis elegans 0.0
blast T25896 hypothetical protein T20F7.4 - Caenorhabditis elegans 1.0E-66
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000037355 1 7-24
Sequence
Target Sequence 
>C. ELEGANS:181396_AT
tcactagtggcatccaatcgccggtacctacgccgccagtcaccaatgaacatgaatcac
agcgatcctctcggctttccgattttacaaatcttgccggaaatagtaataggggaagat
catccagcaatctaggatggtcaagcactactcgaaaagtttatccacgttggagcgaaa
atgaagtatctgcttctagacaagatccgcaaagttcgaatggaagaatcccagggacgt
ctcatagacgtcttccggaaatcccagcgcaattccgaaacaactctggagagagcacat
gggctcagattgtgggttcaagagaaaatgagccaaccgaagctccactccgtcggcggc
atcaatcacaagacagcacaatgcgtgtagacaacggacggtatcgacaatcatggacaa
atgatcacacgcgtttgaggtttattatcccaaccgaaaccgaattcatggctcgggtgc
cgtttccaccgtacagaattccatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCACTAGTGGCATCCAATCGCCGGT 234 623 1520 Antisense
GCCGCCAGTCACCAATGAACATGAA 584 285 1551 Antisense
AACATGAATCACAGCGATCCTCTCG 18 139 1568 Antisense
TCCTCTCGGCTTTCCGATTTTACAA 400 591 1585 Antisense
GAATCCCAGGGACGTCTCATAGACG 529 329 1745 Antisense
CATAGACGTCTTCCGGAAATCCCAG 237 211 1762 Antisense
TCCCAGCGCAATTCCGAAACAACTC 413 595 1781 Antisense
AAATGAGCCAACCGAAGCTCCACTC 11 121 1845 Antisense
CCGTCGGCGGCATCAATCACAAGAC 454 265 1869 Antisense
CGAATTCATGGCTCGGGTGCCGTTT 374 251 1980 Antisense
CGTTTCCACCGTACAGAATTCCATT 460 245 2000 Antisense
Sequence Source Affymetrix Proprietary Database