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[181375_at]

Description K09F6.4 /REP_DB=WormBase Gene ID /WP=CE12030 /TR=O16729 /GB=AAB66194.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 37      6grp_total > 64      4grp_total > 17      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 59
(1)
P 		47.9
(-3)
A 		54.2
(-0.7)
A 		64.5
(3)
A 		17
B 28.5
(-1.2)
A 		40
(3)
A 		23.5
(-3)
A 		23.5
(-3)
A 		17
C 11
(-3)
A 		19.6
(-1.4)
A 		28.7
(0.3)
A 		42.8
(3)
A 		32
avr 32.8
(-3) 		35.8
(-1.3) 		35.5
(-1.5) 		43.6
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 50
(3)
P 		42.3
(0.7)
P 		29.8
(-3)
A 		35.1
(-1.4)
P 		33.1
(-2)
P 		39.2
(-0.2)
P 		20
B 29
(-3)
A 		29.8
(-2.8)
P 		48.7
(3)
P 		33.9
(-1.5)
A 		37
(-0.6)
P 		42.9
(1.2)
P 		20
C 2.1
(-3)
A 		31.8
(1.3)
A 		43.2
(3)
A 		34.3
(1.7)
A 		25.8
(0.5)
P 		31.4
(1.3)
M 		41
avr 27
(-3) 		34.6
(0.4) 		40.6
(3) 		34.4
(0.3) 		32
(-0.8) 		37.8
(1.8) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 21.5
(3)
P 		10.4
(-2.5)
A 		14.2
(-0.6)
A 		9.4
(-3)
A 		12
B 9.1
(2)
A 		9.9
(3)
A 		5.5
(-2.4)
A 		5
(-3)
A 		5
C 3.6
(-3)
A 		5.6
(-1.1)
A 		10
(3)
A 		7.2
(0.4)
A 		6
avr 11.4
(3) 		8.6
(-1) 		9.9
(0.9) 		7.2
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 9.2
(-1.1)
P 		15
(3)
P 		6.5
(-3)
A 		8.9
(-1.3)
P 		10
(-0.5)
P 		9.5
(-0.9)
P 		9
B 10.1
(-2.1)
A 		9.9
(-2.2)
P 		11.8
(-0.6)
P 		9
(-3)
A 		15
(2.1)
P 		16
(3)
P 		7
C 0.8
(-3)
A 		9.7
(2.2)
A 		11
(3)
A 		6.9
(0.6)
A 		8.3
(1.4)
P 		10.5
(2.7)
M 		10
avr 6.7
(-3) 		11.5
(2.4) 		9.8
(0.5) 		8.3
(-1.2) 		11.1
(2) 		12
(3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181375_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5355
Sequence Type Exemplar sequence
Representative Public ID K09F6.4 NCBI
Target Description K09F6.4 /REP_DB=WormBase Gene ID /WP=CE12030 /TR=O16729 /GB=AAB66194.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061821(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061821 NCBI Caenorhabditis elegans K09F6.4 (K09F6.4) mRNA, complete cds. 11/11 None
SNAP00000009215 ENSEMBL cdna:SNAP chromosome:CEL140:II:2262198:2268776:-1 11/11 None
GENEFINDER00000009245 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2262198:2265436:-1 11/11 None
K09F6.4 ENSEMBL cdna:known chromosome:CEL140:II:2262198:2265436:-1 gene:K09F6.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_061820 1/11 Cross Hyb Matching Probes A 
SNAP00000009214 1/11 Cross Hyb Matching Probes None
GENEFINDER00000009246 1/11 Cross Hyb Matching Probes None
K09F6.3 1/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2262196-2265435(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K09F6.4
Entrez Gene 187232 Entrez gene
SwissProt O16729 EMBL-EBI
WormBase CE12030 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66194 Hypothetical protein K09F6.4 [Caenorhabditis elegans] ref|NP_494222.1| K09F6.4 [Caenorhabditis elegans] 0.0
blast AAB66199 Hypothetical protein K09F6.3 [Caenorhabditis elegans] ref|NP_494221.2| K09F6.3 [Caenorhabditis elegans] 0.0
blast AAB66199 Hypothetical protein K09F6.3 [Caenorhabditis elegans] ref|NP_494221.2| K09F6.3 [Caenorhabditis elegans] 1.0E-177
blast CAE58057 Hypothetical protein CBG01136 [Caenorhabditis briggsae] 7.0E-29
blast CAE58057 Hypothetical protein CBG01136 [Caenorhabditis briggsae] 6.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 3.2E-26
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 3.2E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494222.1 1 649-671
K09F6.4 1 649-671
SNAP00000009215 1 803-825
GENEFINDER00000009245 1 783-805
Sequence
Target Sequence 
>C. ELEGANS:181375_AT
gaagcacgacatcacttgcagactatgcacacatctttcaaaatgctttgaaagtcggtg
gagttgatgatgatctgaagaagttgatggatgcagttgaggatctcgagaatagtgtga
cagatggtgcaacgaaggtcaaactgaaggaggtgaaagcatctctcagctctctggact
ccattggtctgcaactttctagctttgccactccatttattgcaattgggcagtcgttta
ctgaacttgatgcattctttgtcagcaatcgaaagatcatcactacaactgctccgattg
tttctagttctgacgcttctcaaggaggacagaaagcttctggcggatcgatgactgtgc
agccatatcagaatgtatctggtacaactactgcagaacctatgcacgagtcaacaaatt
tgataatgttcattggaatcggatgtggaattgtcctggtgtcagcgttgattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCACGACATCACTTGCAGACTA 424 341 1535 Antisense
TGCAGACTATGCACACATCTTTCAA 469 577 1551 Antisense
GAGGTGAAAGCATCTCTCAGCTCTC 35 403 1684 Antisense
TCTCTGGACTCCATTGGTCTGCAAC 462 611 1705 Antisense
GGTCTGCAACTTTCTAGCTTTGCCA 272 503 1720 Antisense
TAGCTTTGCCACTCCATTTATTGCA 309 651 1734 Antisense
AAAGATCATCACTACAACTGCTCCG 388 123 1806 Antisense
GCTCCGATTGTTTCTAGTTCTGACG 419 297 1825 Antisense
AGTTCTGACGCTTCTCAAGGAGGAC 71 63 1840 Antisense
GCTTCTGGCGGATCGATGACTGTGC 256 305 1870 Antisense
ATTGTCCTGGTGTCAGCGTTGATTT 370 3 1984 Antisense
Sequence Source Affymetrix Proprietary Database