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[181355_s_at]

Description D1054.14 /REP_DB=WormBase Gene ID /WP=CE05532 /TR=Q18942 /GB=CAA98434.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_highest_8_8 > 27      4grp_high_8_8 > 56      6grp_highest_nonorm > 94      6grp_total > 87      4grp_total > 63      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 249
1 2 3 4 range
A 432.5
(3)
P 		397
(1.5)
P 		400
(1.7)
P 		286.9
(-3)
P 		146
B 438.3
(0.3)
P 		398.5
(-3)
P 		432.6
(-0.2)
P 		471.4
(3)
P 		73
C 482.7
(1.2)
P 		359.9
(-3)
P 		536.1
(3)
P 		418.6
(-1)
P 		176
avr 451.2
(2.6) 		385.1
(-3) 		456.2
(3) 		392.3
(-2.4) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 642
1 2 3 4 5 6 range
A 482.9
(-1.8)
P 		401.6
(-3)
P 		475.8
(-1.9)
P 		560.9
(-0.7)
P 		625.3
(0.2)
P 		823.1
(3)
P 		422
B 442.1
(-2.3)
P 		363.9
(-3)
P 		544.6
(-1.3)
P 		580.2
(-0.9)
P 		695
(0.1)
P 		995.2
(3)
P 		631
C 410
(-2.9)
P 		437.5
(-2.6)
P 		467.4
(-2.3)
P 		400.2
(-3)
P 		603.9
(-1)
P 		1006
(3)
P 		606
avr 445
(-2.5) 		401
(-3) 		495.9
(-1.9) 		513.8
(-1.7) 		641.4
(-0.3) 		941.4
(3) 		540
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 157.5
(3)
P 		86.2
(-0.7)
P 		104.6
(0.3)
P 		42
(-3)
P 		116
B 139.8
(3)
P 		98.4
(-3)
P 		100.3
(-2.7)
P 		100.5
(-2.7)
P 		41
C 159.8
(1.6)
P 		103.5
(-1.3)
P 		186.9
(3)
P 		70.6
(-3)
P 		116
avr 152.4
(3) 		96
(-1.2) 		130.6
(1.4) 		71
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 89
(-3)
P 		142.8
(-0.1)
P 		103.6
(-2.2)
P 		142.2
(-0.1)
P 		188.7
(2.4)
P 		200.4
(3)
P 		111
B 153.8
(-2.2)
P 		121.1
(-3)
P 		132.3
(-2.7)
P 		153.7
(-2.2)
P 		282.4
(0.9)
P 		371.1
(3)
P 		250
C 152
(-1.3)
P 		133.5
(-1.8)
P 		119
(-2.1)
P 		80.5
(-3)
P 		195.4
(-0.3)
P 		336.8
(3)
P 		256
avr 131.6
(-2.6) 		132.5
(-2.5) 		118.3
(-3) 		125.5
(-2.8) 		222.2
(0.4) 		302.8
(3) 		185
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181355_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13124
Sequence Type Exemplar sequence
Representative Public ID D1054.14 NCBI
Target Description D1054.14 /REP_DB=WormBase Gene ID /WP=CE05532 /TR=Q18942 /GB=CAA98434.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073361(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073361 NCBI Caenorhabditis elegans D1054.14 (D1054.14) mRNA, complete cds. 11/11 None
SNAP00000017228 ENSEMBL cdna:SNAP chromosome:CEL140:V:10807428:10808620:-1 11/11 None
GENEFINDER00000017235 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10807428:10808620:-1 11/11 None
D1054.14.1 ENSEMBL cdna:known chromosome:CEL140:V:10807376:10808626:-1 gene:D1054.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10807427-10808620(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl D1054.14
Entrez Gene 179499 Entrez gene
SwissProt Q18942 EMBL-EBI
WormBase CE05532 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:266055_AT pre-mRNA splicing factor PRP38 family protein at
CANINE_2:CFA.17125.1.S1_AT similar to PRP38 pre-mRNA processing factor 38 (yeast) domain containing A isoform 2 cfa
CANINE_2:CFAAFFX.6554.1.S1_S_AT similar to PRP38 pre-mRNA processing factor 38 (yeast) domain containing A isoform 2 cfa
DROSOPHILA_2:1638997_AT dm
HU35KSUBA:RC_AA262655_S_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
HU35KSUBD:RC_T16707_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
U133_X3P:HS2.5301.2.S1_3P_S_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
U133_X3P:HS.5301.0.S2_3P_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
HG-U133_PLUS_2:1553709_A_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
HG-U133_PLUS_2:223230_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
HG-U133B:223230_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
HG-U95E:83593_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
HG-U95E:87910_S_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
HU35KSUBC:RC_D25575_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A hs
MU11KSUBA:AA254074_S_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A mm
MG-U74AV2:104713_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A mm
MOE430A:1433565_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A mm
MOUSE430A_2:1433565_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A mm
MOUSE430_2:1433565_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A mm
MOE430B:1444087_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A mm
MOUSE430_2:1444087_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A mm
MOE430B:1443282_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A, mRNA (cDNA clone IMAGE:6810590) mm
MOUSE430_2:1443282_AT PRP38 pre-mRNA processing factor 38 (yeast) domain containing A, mRNA (cDNA clone IMAGE:6810590) mm
RG-U34C:RC_AI236727_AT similar to hypothetical protein FLJ14936 (predicted) rn
RAE230A:1373273_AT similar to hypothetical protein FLJ14936 (predicted) rn
RAT230_2:1373273_AT similar to hypothetical protein FLJ14936 (predicted) rn
RG-U34B:RC_AI030220_F_AT Similar to hypothetical protein FLJ14936 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA98434 Hypothetical protein D1054.14 [Caenorhabditis elegans] ref|NP_505762.1| D1054.14 [Caenorhabditis elegans] 1.0E-118
blast CAE64898 Hypothetical protein CBG09713 [Caenorhabditis briggsae] 1.0E-114
blast XP_397219 PREDICTED: similar to ENSANGP00000012048 [Apis mellifera] 2.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR005037 EMBL-EBI PRP38 family 2.5E-109
Sequence
Target Sequence 
>C. ELEGANS:181355_S_AT
gggtcgattcgaagctatctacatggacgatttcatcgacaatttacttcgtgaagatcg
ctattgtgacattcaacttcctcgtctacagaagagatgggctcttgaagaagtagacat
gcttccgtcatataagtcacttcttgatggtgatcttgttgcaatgagtgattctgattc
tgaagaggaagaagtcacaaagaaggagaagccacgtttgacatctagaagacgtagtcg
aagcagagatcgcgaaagagatgttggtgatcgacgagaagttcgtgaacgagaaaaact
gaaagaacgaagagaaagaggagacgatgagccagggccaagcagtagtggtagtggtag
acgtgatgatagagatgacagaagaagagatcgtgatagaagccgggatagagatcgtcg
agatagaagagatgatgatcgtcgtgataagaaaaaagaatctcgtcgaggtggtgcaga
caacgatgaagaacgcgaaatcgcagaagcgaatgcactccgtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTCGATTCGAAGCTATCTACATG 525 493 420 Antisense
GACGATTTCATCGACAATTTACTTC 249 379 445 Antisense
GATCGCTATTGTGACATTCAACTTC 591 417 475 Antisense
CATTCAACTTCCTCGTCTACAGAAG 450 213 489 Antisense
GACATGCTTCCGTCATATAAGTCAC 589 369 535 Antisense
GGAGAAGCCACGTTTGACATCTAGA 180 519 624 Antisense
AGAGGAGACGATGAGCCAGGGCCAA 422 65 736 Antisense
GAGCCAGGGCCAAGCAGTAGTGGTA 297 385 748 Antisense
GAAGCCGGGATAGAGATCGTCGAGA 69 345 818 Antisense
GAATCTCGTCGAGGTGGTGCAGACA 416 329 877 Antisense
TCGCAGAAGCGAATGCACTCCGTGC 326 599 920 Antisense
Sequence Source Affymetrix Proprietary Database