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[181353_s_at]

Description Y53F4B.9 /REP_DB=WormBase Gene ID /WP=CE22399 /TR=Q9NAC1 /GB=CAB61080.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 79      6grp_total > 79      4grp_total > 60      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 95.3
(1.4)
A 		98.3
(1.6)
P 		126.2
(3)
P 		8.7
(-3)
A 		118
B 75
(-0.7)
P 		60
(-3)
A 		99.9
(3)
P 		94.6
(2.2)
A 		40
C 76.7
(1.5)
P 		77.7
(1.5)
A 		101.3
(3)
P 		5.8
(-3)
A 		96
avr 82.3
(0.8) 		78.7
(0.5) 		109.1
(3) 		36.4
(-3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 211
1 2 3 4 5 6 range
A 140.5
(-1.1)
A 		126.8
(-1.5)
P 		100.7
(-2.2)
A 		74.5
(-3)
A 		173.7
(-0.1)
P 		283.1
(3)
P 		209
B 116.1
(-1.1)
P 		107.6
(-1.5)
P 		123.9
(-0.7)
M 		72.5
(-3)
P 		123.9
(-0.7)
P 		208.9
(3)
P 		136
C 101
(-1)
P 		73.1
(-3)
P 		82.9
(-2.3)
P 		72.6
(-3)
A 		135
(1.3)
P 		159.5
(3)
P 		87
avr 119.2
(-1.1) 		102.5
(-1.8) 		102.5
(-1.8) 		73.2
(-3) 		144.2
(-0) 		217.2
(3) 		144
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 34.7
(3)
A 		21.3
(0.6)
P 		33
(2.7)
P 		1.3
(-3)
A 		33
B 23.9
(3)
P 		14.8
(-3)
A 		23.2
(2.5)
P 		20.2
(0.6)
A 		9
C 25.4
(1.3)
P 		22.3
(0.7)
A 		35.3
(3)
P 		1
(-3)
A 		34
avr 28
(2.3) 		19.5
(0.1) 		30.5
(3) 		7.5
(-3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 25.9
(-2.2)
A 		45.1
(0.1)
P 		21.9
(-2.6)
A 		18.9
(-3)
A 		52.4
(1)
P 		68.9
(3)
P 		50
B 40.4
(-0.8)
P 		35.8
(-1.3)
P 		30.1
(-1.9)
M 		19.2
(-3)
P 		50.4
(0.2)
P 		77.9
(3)
P 		59
C 37.4
(0.5)
P 		22.3
(-1.8)
P 		21.1
(-2)
P 		14.6
(-3)
A 		43.7
(1.5)
P 		53.4
(3)
P 		39
avr 34.6
(-0.9) 		34.4
(-0.9) 		24.4
(-2.2) 		17.6
(-3) 		48.8
(0.8) 		66.7
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181353_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2735
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.9 NCBI
Target Description Y53F4B.9 /REP_DB=WormBase Gene ID /WP=CE22399 /TR=Q9NAC1 /GB=CAB61080.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064694(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064694 NCBI Caenorhabditis elegans Y53F4B.9 (Y53F4B.9) mRNA, complete cds. 11/11 None
SNAP00000043045 ENSEMBL cdna:SNAP chromosome:CEL140:II:14999703:15012515:-1 11/11 None
GENEFINDER00000043117 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15001854:15010301:-1 11/11 None
Y53F4B.9 ENSEMBL cdna:known chromosome:CEL140:II:15004773:15010301:-1 gene:Y53F4B.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15004910-15010298(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.9
Entrez Gene 175155 Entrez gene
SwissProt Q9NAC1 EMBL-EBI
WormBase CE22399 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61080 Hypothetical protein Y53F4B.9 [Caenorhabditis elegans] ref|NP_497095.1| Y53F4B.9 [Caenorhabditis elegans] 0.0
blast CAE73538 Hypothetical protein CBG21002 [Caenorhabditis briggsae] 0.0
blast CAB61079 Hypothetical protein Y53F4B.10 [Caenorhabditis elegans] ref|NP_497094.1| Y53F4B.10 [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:181353_S_AT
tgtacagaatctcgtctactcgtgccgtaacgtgattatcgacgacgcgacgatcaagca
gctctccgagtccgtgaatcacgatctgagtgctctcctccatcaagctcatcttttctc
gctggctccacaccgcccattcccacttacaactcgccatctcgtttcaaccggattctc
cgagcactgggaaaagccacgttgcagcttaaccaagacacaggaacgaatggaggagct
cttcgagcagtacgcggatgttctcccagacggagcagtggctctgagccgctcaccggg
agtggagaaactatgggatacggatcgccagaagctcgcggaatctcaggctggatattc
ggaaggtgtccggaacgcttacgaggctctcaatggacgattttcgcggcgagacctggt
tcaagatgtgctcccgatgctcgtgccaatggatcgtgtcgaacgtcagaaacgggtccg
atcacggcgacacttgcatcgattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTACAGAATCTCGTCTACTCGTGC 508 559 2937 Antisense
TGAATCACGATCTGAGTGCTCTCCT 19 577 3011 Antisense
TCAACCGGATTCTCCGAGCACTGGG 584 629 3103 Antisense
AGCCACGTTGCAGCTTAACCAAGAC 232 83 3131 Antisense
AGCTCTTCGAGCAGTACGCGGATGT 321 79 3173 Antisense
ACGGAGCAGTGGCTCTGAGCCGCTC 548 95 3206 Antisense
ATACGGATCGCCAGAAGCTCGCGGA 136 23 3254 Antisense
AGCTCGCGGAATCTCAGGCTGGATA 167 81 3269 Antisense
GGAACGCTTACGAGGCTCTCAATGG 233 529 3308 Antisense
AGACCTGGTTCAAGATGTGCTCCCG 358 71 3348 Antisense
ATCACGGCGACACTTGCATCGATTC 137 29 3417 Antisense
Sequence Source Affymetrix Proprietary Database