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[181340_at]

Description T16D1.2 /REP_DB=WormBase Gene ID /WP=CE04969 /TR=Q22525 /GB=AAA80132.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 05      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 11      6grp_total > 14      4grp_total > 55      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2238
1 2 3 4 range
A 2491.1
(3)
P 		661.6
(-2)
P 		722.8
(-1.8)
P 		303.5
(-3)
P 		2188
B 2517.5
(3)
P 		756.6
(-1.7)
P 		805.9
(-1.6)
P 		280.8
(-3)
P 		2237
C 2518.4
(3)
P 		839.2
(-1.6)
P 		936.2
(-1.3)
P 		310.5
(-3)
P 		2208
avr 2509
(3) 		752.5
(-1.8) 		821.6
(-1.6) 		298.3
(-3) 		2211

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2055
1 2 3 4 5 6 range
A 2558.3
(3)
P 		1648.8
(0.3)
P 		1448.2
(-0.2)
P 		1348.3
(-0.5)
P 		931.2
(-1.8)
P 		503.2
(-3)
P 		2055
B 2311.9
(3)
P 		1874.6
(1.5)
P 		1440.1
(0)
P 		1350.2
(-0.3)
P 		1331.2
(-0.3)
P 		551.5
(-3)
P 		1760
C 2078.3
(3)
P 		1836.4
(2)
P 		1402.7
(0.2)
P 		1155
(-0.8)
P 		873
(-2)
P 		625.8
(-3)
P 		1453
avr 2316.2
(3) 		1786.6
(1.2) 		1430.3
(-0) 		1284.5
(-0.5) 		1045.1
(-1.3) 		560.2
(-3) 		1756
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 863
1 2 3 4 range
A 907
(3)
P 		143.6
(-2.3)
P 		189
(-2)
P 		44.4
(-3)
P 		863
B 802.8
(3)
P 		186.9
(-2)
P 		186.8
(-2)
P 		59.8
(-3)
P 		743
C 833.7
(3)
P 		241.3
(-1.5)
P 		326.3
(-0.9)
P 		52.4
(-3)
P 		781
avr 847.8
(3) 		190.6
(-2) 		234
(-1.6) 		52.2
(-3) 		796

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 682
1 2 3 4 5 6 range
A 471.6
(1.5)
P 		586.4
(3)
P 		315.4
(-0.5)
P 		342
(-0.2)
P 		281
(-0.9)
P 		122.5
(-3)
P 		464
B 804.4
(3)
P 		624
(1.2)
P 		349.7
(-1.6)
P 		357.6
(-1.5)
P 		540.8
(0.4)
P 		205.6
(-3)
P 		599
C 770.3
(3)
P 		560.3
(0.8)
P 		357
(-1.4)
P 		232.3
(-2.8)
P 		282.5
(-2.2)
P 		209.5
(-3)
P 		561
avr 682.1
(3) 		590.2
(1.9) 		340.7
(-1.1) 		310.6
(-1.4) 		368.1
(-0.7) 		179.2
(-3) 		503
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181340_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4357
Sequence Type Exemplar sequence
Representative Public ID T16D1.2 NCBI
Target Description T16D1.2 /REP_DB=WormBase Gene ID /WP=CE04969 /TR=Q22525 /GB=AAA80132.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062583(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062583 NCBI Caenorhabditis elegans intestinal acid PHOsphatase family member (pho-4) (pho-4) mRNA, complete cds. 10/11 None
SNAP00000026375 ENSEMBL cdna:SNAP chromosome:CEL140:II:5236286:5238435:-1 10/11 None
GENEFINDER00000026380 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5236240:5238435:-1 10/11 None
T16D1.2 ENSEMBL cdna:known chromosome:CEL140:II:5236237:5238435:-1 gene:T16D1.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5236709-5238433(-) 99.56 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pho-4
Ensembl T16D1.2
Entrez Gene 173897 Entrez gene
SwissProt Q22525 EMBL-EBI
WormBase CE36570 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3993 acid phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA80132 Intestinal acid phosphatase protein 4 [Caenorhabditis elegans] ref|NP_494984.4| intestinal acid PHOsphatase family member (pho-4) [Caenorhabditis elegans] 0.0
blast CAE59071 Hypothetical protein CBG02357 [Caenorhabditis briggsae] 0.0
blast AAA83593 Intestinal acid phosphatase protein 1 [Caenorhabditis elegans] ref|NP_494983.1| intestinal acid PHOsphatase family member (pho-1) [Caenorhabditis elegans] 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 5.7E-18
Pfam IPR000560 EMBL-EBI Histidine acid phosphatase 4.4E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000026380 1 449-471
Sequence
Target Sequence 
>C. ELEGANS:181340_AT
atcatcttctatccgtcgataactactgtccacttcaagacacaatttggaatcttgcca
aaacaacagacacagtatcgaattatttcaacagttctgctgtaacatcgcttatgggaa
atctaacaaattattgcggagaagagattaatccagataatttatggattctttataatg
cacttaagattgagaaacaatattatccagctcagttcaaacaattcacaccatggtaca
ctgacagtttgtttgagcaaattgatattgtcaattcgcaggttcaagattttcaaaatg
gtttgggtttggaaggaaaaatggtaaacggaatcgacattggaaagattcttcgaaaga
ttcgtggtgggactctagtcaatgacatctacaatcatatgaataggaaaacgcaatgtt
catcaaataatggaaaagagtgtacttacacgaatcgattgaaatttttcgcttattcag
cacacgacacaaccctctacgcccttttttcattgctcggtgtcgccaaattgctgtgca
gccacgtggatatcctctctattctgcatgtgctctttttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCATCTTCTATCCGTCGATAACTA 593 29 530 Antisense
TCGATAACTACTGTCCACTTCAAGA 455 603 545 Antisense
ACAGTTCTGCTGTAACATCGCTTAT 394 107 620 Antisense
ACAATATTATCCAGCTCAGTTCAAA 209 115 726 Antisense
TCAGTTCAAACAATTCACACCATGG 309 623 741 Antisense
CACACCATGGTACACTGACAGTTTG 411 195 756 Antisense
AGATTCGTGGTGGGACTCTAGTCAA 622 63 887 Antisense
TTTTCGCTTATTCAGCACACGACAC 487 675 995 Antisense
ATTGCTGTGCAGCCACGTGGATATC 600 5 1059 Antisense
CACGTGGATATCCTCTCTATTCTGC 522 199 1072 Antisense
TCTATTCTGCATGTGCTCTTTTTGA 364 615 1087 Antisense
Sequence Source Affymetrix Proprietary Database