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[181339_s_at]

Description K09F6.5 /REP_DB=WormBase Gene ID /WP=CE12032 /TR=O16731 /GB=AAB66195.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 17      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 22.3
(-2.9)
A 		21
(-3)
A 		54.7
(0)
A 		88.3
(3)
A 		67
B 4.8
(-3)
A 		45.6
(2.7)
A 		17.6
(-1.2)
A 		48
(3)
A 		43
C 11.9
(-2.2)
A 		13.2
(-2.1)
A 		4.3
(-3)
A 		64.3
(3)
A 		60
avr 13
(-3) 		26.6
(-1.5) 		25.5
(-1.6) 		66.9
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 3.4
(-3)
A 		38.9
(1)
A 		5
(-2.8)
A 		37.6
(0.8)
A 		7.1
(-2.6)
A 		56.7
(3)
A 		53
B 3.9
(-3)
A 		13.2
(-0.9)
A 		6.5
(-2.4)
A 		31
(3)
A 		18.4
(0.2)
A 		4.7
(-2.8)
A 		27
C 33.9
(1.5)
A 		6.5
(-2.4)
A 		2.2
(-3)
A 		44.1
(3)
A 		32.2
(1.3)
A 		6
(-2.5)
A 		42
avr 13.7
(-1.3) 		19.5
(-0.3) 		4.6
(-3) 		37.6
(3) 		19.2
(-0.3) 		22.5
(0.3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 8.1
(-0.8)
A 		4.6
(-3)
A 		14.3
(3)
A 		12.9
(2.1)
A 		10
B 1.5
(-3)
A 		11.3
(3)
A 		4.1
(-1.4)
A 		10.2
(2.3)
A 		10
C 3.9
(-1.5)
A 		3.8
(-1.5)
A 		1.5
(-3)
A 		10.8
(3)
A 		9
avr 4.5
(-3) 		6.6
(-1.1) 		6.6
(-1.1) 		11.3
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 0.6
(-3)
A 		13.9
(3)
A 		1.1
(-2.8)
A 		9.5
(1)
A 		2.1
(-2.3)
A 		13.8
(3)
A 		13
B 1.3
(-3)
A 		4.4
(-0.3)
A 		1.6
(-2.7)
A 		8.2
(3)
A 		7.5
(2.4)
A 		1.8
(-2.6)
A 		7
C 12.6
(3)
A 		2
(-2.3)
A 		0.6
(-3)
A 		8.9
(1.2)
A 		10.4
(1.9)
A 		2
(-2.3)
A 		12
avr 4.8
(-0.2) 		6.8
(1.4) 		1.1
(-3) 		8.9
(3) 		6.7
(1.3) 		5.9
(0.7) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181339_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5725
Sequence Type Exemplar sequence
Representative Public ID K09F6.5 NCBI
Target Description K09F6.5 /REP_DB=WormBase Gene ID /WP=CE12032 /TR=O16731 /GB=AAB66195.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066334(11), NM_061823(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066334 NCBI Caenorhabditis elegans F54H12.2 (F54H12.2) mRNA, complete cds. 11/11 A 
NM_061823 NCBI Caenorhabditis elegans K09F6.5 (K09F6.5) mRNA, complete cds. 11/11 A 
SNAP00000009218 ENSEMBL cdna:SNAP chromosome:CEL140:II:2272063:2273385:-1 11/11 None
GENEFINDER00000009237 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2272063:2273385:-1 11/11 None
SNAP00000022459 ENSEMBL cdna:SNAP chromosome:CEL140:III:7962935:7965764:1 11/11 None
GENEFINDER00000022470 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7964505:7965764:1 11/11 None
K09F6.5 ENSEMBL cdna:known chromosome:CEL140:II:2272063:2273385:-1 gene:K09F6.5 11/11 A 
F54H12.2 ENSEMBL cdna:known chromosome:CEL140:III:7964505:7965764:1 gene:F54H12.2 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006525 1/11 Cross Hyb Matching Probes None
GENEFINDER00000006535 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2272061-2273384(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl K09F6.5
F54H12.2
Entrez Gene 186266 Entrez gene
187233 Entrez gene
SwissProt P34456 EMBL-EBI
O16731 EMBL-EBI
WormBase CE00515 Wormbase
CE12032 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84536 Hypothetical protein F54H12.2 [Caenorhabditis elegans] ref|NP_498735.1| F54H12.2 [Caenorhabditis elegans] sp|P34456|YMD2_CAEEL Hypothetical protein F54H12.2 in chromosome III 0.0
blast AAB66195 Hypothetical protein K09F6.5 [Caenorhabditis elegans] ref|NP_494224.1| K09F6.5 [Caenorhabditis elegans] 0.0
blast AAG24065 Hypothetical protein F19G12.2 [Caenorhabditis elegans] ref|NP_508269.1| F19G12.2 [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:181339_S_AT
aacgagttggaggctgcaatcattgaacacaagatgatccaatacccaatgatttcctca
caagttcgcagttgctacattgatggagggcggtttgacgcccctgcaaatacgttgttc
actagtaaaatgcctcgtcgactctttctgggtctagtttcaagcgaagcttacaacgga
tcattcggaacaactccgttcaacttcaaaccatatggcgtgcaggacgttcacattgat
tattgcggtcaaacaattccaggacgaccaatgaatctagactttgagaataacaaatct
aaaggatacacgatttttggattcgagttgagccctgtggctcaggataataatcttttc
gaactagttcgtcaaacaaacgttagcgtacgcctcaatttcaaaaaggaaactccacca
ggaggtctctactgcatcgtctacgccgaatttgatcaaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGAGTTGGAGGCTGCAATCATTG 441 145 736 Antisense
ACATTGATGGAGGGCGGTTTGACGC 356 103 812 Antisense
GACTCTTTCTGGGTCTAGTTTCAAG 637 373 875 Antisense
CTGGGTCTAGTTTCAAGCGAAGCTT 62 239 883 Antisense
GGATTCGAGTTGAGCCCTGTGGCTC 382 509 1054 Antisense
GAGCCCTGTGGCTCAGGATAATAAT 434 385 1065 Antisense
TCGAACTAGTTCGTCAAACAAACGT 87 545 1094 Antisense
CAAACAAACGTTAGCGTACGCCTCA 359 189 1108 Antisense
TTAGCGTACGCCTCAATTTCAAAAA 680 679 1118 Antisense
AGGAAACTCCACCAGGAGGTCTCTA 516 55 1142 Antisense
CGTCTACGCCGAATTTGATCAAATA 145 247 1173 Antisense
Sequence Source Affymetrix Proprietary Database