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[181338_at]

Description K09F6.5 /REP_DB=WormBase Gene ID /WP=CE12032 /TR=O16731 /GB=AAB66195.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 50      4grp_total > 37      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 3.4
(-1.4)
A 		7.1
(3)
A 		2.1
(-3)
A 		3.2
(-1.7)
A 		5
B 6.2
(3)
A 		5.4
(1.9)
A 		2.6
(-2)
A 		1.9
(-3)
A 		4
C 12.1
(3)
A 		4.3
(-2)
A 		2.8
(-3)
A 		9.5
(1.3)
A 		9
avr 7.2
(3) 		5.6
(1) 		2.5
(-3) 		4.9
(0.1) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 26.8
(3)
A 		6.2
(-2.3)
A 		4.9
(-2.7)
A 		3.6
(-3)
A 		4.3
(-2.8)
A 		6.6
(-2.2)
A 		23
B 4.9
(-1.8)
A 		2.7
(-3)
A 		13.4
(2.7)
A 		13.9
(3)
A 		2.9
(-2.9)
A 		8
(-0.2)
A 		11
C 4.2
(-3)
A 		13.7
(-1.2)
A 		23
(0.6)
A 		35.4
(3)
A 		4.6
(-2.9)
A 		4.2
(-3)
A 		31
avr 12
(0.5) 		7.5
(-1.4) 		13.8
(1.3) 		17.6
(3) 		3.9
(-3) 		6.3
(-1.9) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 1.2
(1.2)
A 		1.5
(3)
A 		0.6
(-2.4)
A 		0.5
(-3)
A 		1
B 2
(3)
A 		1.3
(0.4)
A 		0.6
(-2.3)
A 		0.4
(-3)
A 		2
C 4
(3)
A 		1.2
(-2.6)
A 		1
(-3)
A 		1.6
(-1.8)
A 		3
avr 2.4
(3) 		1.3
(-0.9) 		0.7
(-3) 		0.8
(-2.6) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 4.9
(3)
A 		2.2
(-1.1)
A 		1.1
(-2.7)
A 		0.9
(-3)
A 		1.3
(-2.4)
A 		1.6
(-2)
A 		4
B 1.7
(-1.3)
A 		0.9
(-3)
A 		3.2
(1.9)
A 		3.7
(3)
A 		1.2
(-2.4)
A 		3
(1.5)
A 		3
C 1.5
(-2.9)
A 		4.2
(-0.1)
A 		5.9
(1.7)
A 		7.1
(3)
A 		1.5
(-2.9)
A 		1.4
(-3)
A 		6
avr 2.7
(0.2) 		2.4
(-0.5) 		3.4
(1.8) 		3.9
(3) 		1.3
(-3) 		2
(-1.4) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181338_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5725
Sequence Type Exemplar sequence
Representative Public ID K09F6.5 NCBI
Target Description K09F6.5 /REP_DB=WormBase Gene ID /WP=CE12032 /TR=O16731 /GB=AAB66195.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061823(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061823 NCBI Caenorhabditis elegans K09F6.5 (K09F6.5) mRNA, complete cds. 11/11 A 
SNAP00000009218 ENSEMBL cdna:SNAP chromosome:CEL140:II:2272063:2273385:-1 11/11 None
GENEFINDER00000009237 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2272063:2273385:-1 11/11 None
K09F6.5 ENSEMBL cdna:known chromosome:CEL140:II:2272063:2273385:-1 gene:K09F6.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000006525 2/11 Cross Hyb Matching Probes None
GENEFINDER00000006535 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2272061-2273384(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K09F6.5
Entrez Gene 187233 Entrez gene
SwissProt O16731 EMBL-EBI
WormBase CE12032 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66195 Hypothetical protein K09F6.5 [Caenorhabditis elegans] ref|NP_494224.1| K09F6.5 [Caenorhabditis elegans] 0.0
blast AAK84536 Hypothetical protein F54H12.2 [Caenorhabditis elegans] ref|NP_498735.1| F54H12.2 [Caenorhabditis elegans] sp|P34456|YMD2_CAEEL Hypothetical protein F54H12.2 in chromosome III 0.0
blast AAG24065 Hypothetical protein F19G12.2 [Caenorhabditis elegans] ref|NP_508269.1| F19G12.2 [Caenorhabditis elegans] 1.0E-174
Sequence
Target Sequence 
>C. ELEGANS:181338_AT
gttactgatgtttacgctcttgttaatgagtttgatctaacagatggtttgtcaaacgag
ttggaggctgcaatcattgaacacaagatgatccaatacccaatgatttcctcacaagtt
cgcagttgctacattgatggagggcggtttgacgcccctgcaaatacgttgttcactagt
aaaatgcctcgtcgactctttctgggtctagtttcaagcgaagcttacaacggatcattc
ggaacaactccgttcaacttcaaaccatatggcgtgcaggacgttcacattgattattgc
ggtcaaacaattccaggacgaccaatgaatctagacttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTACTGATGTTTACGCTCTTGTTA 503 445 682 Antisense
GATGATCCAATACCCAATGATTTCC 447 411 768 Antisense
ATGATTTCCTCACAAGTTCGCAGTT 613 45 784 Antisense
TTCGCAGTTGCTACATTGATGGAGG 396 697 800 Antisense
ACGCCCCTGCAAATACGTTGTTCAC 373 91 833 Antisense
GTAAAATGCCTCGTCGACTCTTTCT 633 461 860 Antisense
AAGCTTACAACGGATCATTCGGAAC 697 149 902 Antisense
ACAACTCCGTTCAACTTCAAACCAT 352 111 925 Antisense
ATGGCGTGCAGGACGTTCACATTGA 333 53 950 Antisense
TGCGGTCAAACAATTCCAGGACGAC 574 553 979 Antisense
AGGACGACCAATGAATCTAGACTTT 493 55 996 Antisense
Sequence Source Affymetrix Proprietary Database