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[181335_at]

Description F53G2.2 /REP_DB=WormBase Gene ID /WP=CE10992 /TR=O17247 /GB=AAB71012.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 31      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 25.6
(3)
A 		4.2
(-3)
A 		4.7
(-2.9)
A 		7.1
(-2.2)
A 		21
B 31
(3)
A 		6.9
(-2.7)
A 		5.6
(-3)
A 		18.8
(0.1)
A 		25
C 2.9
(-3)
A 		7.5
(3)
A 		5
(-0.3)
A 		7
(2.3)
A 		5
avr 19.8
(3) 		6.2
(-2.6) 		5.1
(-3) 		11
(-0.6) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 4.7
(-1.5)
A 		6
(-1)
A 		1.5
(-3)
A 		14.7
(3)
A 		6.9
(-0.5)
A 		5.5
(-1.2)
A 		13
B 3.4
(-2.6)
A 		16
(3)
A 		4.9
(-2)
A 		6.5
(-1.3)
A 		5.8
(-1.6)
A 		2.6
(-3)
A 		13
C 5.7
(-1.9)
A 		1.5
(-3)
A 		9.4
(-1)
A 		12.3
(-0.3)
A 		25.1
(3)
A 		11.3
(-0.5)
A 		24
avr 4.6
(-3) 		7.8
(-0.6) 		5.3
(-2.5) 		11.2
(2) 		12.6
(3) 		6.5
(-1.6) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 9.3
(3)
A 		0.9
(-3)
A 		1.2
(-2.8)
A 		1
(-2.9)
A 		8
B 9.9
(3)
A 		1.7
(-2.7)
A 		1.3
(-3)
A 		4
(-1.1)
A 		9
C 1
(-3)
A 		2.1
(3)
A 		1.8
(1.4)
A 		1.2
(-1.9)
A 		1
avr 6.7
(3) 		1.6
(-2.8) 		1.4
(-3) 		2.1
(-2.2) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.9
(-1.9)
A 		2.1
(0.2)
A 		0.3
(-3)
A 		3.7
(3)
A 		2.1
(0.2)
A 		1.3
(-1.2)
A 		3
B 1.2
(-2.7)
A 		5.3
(3)
A 		1.2
(-2.7)
A 		1.7
(-2)
A 		2.3
(-1.2)
A 		1
(-3)
A 		4
C 2.1
(-1.7)
A 		0.4
(-3)
A 		2.4
(-1.4)
A 		2.5
(-1.4)
A 		8.1
(3)
A 		3.8
(-0.4)
A 		8
avr 1.4
(-2.8) 		2.6
(-0.3) 		1.3
(-3) 		2.6
(-0.3) 		4.2
(3) 		2
(-1.6) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181335_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4392
Sequence Type Exemplar sequence
Representative Public ID F53G2.2 NCBI
Target Description F53G2.2 /REP_DB=WormBase Gene ID /WP=CE10992 /TR=O17247 /GB=AAB71012.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061881(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061881 NCBI Caenorhabditis elegans F53G2.2 (F53G2.2) mRNA, complete cds. 11/11 None
SNAP00000030756 ENSEMBL cdna:SNAP chromosome:CEL140:II:2455148:2485903:1 11/11 None
GENEFINDER00000030763 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2480400:2485903:1 11/11 None
F53G2.2 ENSEMBL cdna:known chromosome:CEL140:II:2481939:2485903:1 gene:F53G2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2481937-2485902(+) 92.54 100.0
Public Domain and Genome References
Gene Title
Ensembl F53G2.2
Entrez Gene 186185 Entrez gene
SwissProt O17247 EMBL-EBI
WormBase CE36862 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71012 Hypothetical protein F53G2.2 [Caenorhabditis elegans] ref|NP_494282.2| F53G2.2 [Caenorhabditis elegans] 0.0
blast AAB71013 Hypothetical protein F53G2.3 [Caenorhabditis elegans] ref|NP_494281.2| F53G2.3 [Caenorhabditis elegans] 1.0E-50
blast AAB71013 Hypothetical protein F53G2.3 [Caenorhabditis elegans] ref|NP_494281.2| F53G2.3 [Caenorhabditis elegans] 4.0E-50
blast AAB71013 Hypothetical protein F53G2.3 [Caenorhabditis elegans] ref|NP_494281.2| F53G2.3 [Caenorhabditis elegans] 1.0E-30
Sequence
Target Sequence 
>C. ELEGANS:181335_AT
gtgggaaaatggctcgtccttgtcatgcacagaaatcctcgtgaaagatgataatcaaac
aacgattctttgtatggattttgtgattagagaacggcgacacgataagtacaaacgagg
atacaagagcttcgacaccaaagcggattttttaaaaggctatcacgtaatagaggaaat
gtgcaaattctgctgcaatggaactgttgcaaagtatcaaattccacaggaactcgcttc
tgtttctctaatgatgcgtgaaaatcacattgatagatttgaaatcggttctgttcacag
cttttacattgcgtccgtaatgaaatccaacaaaagatatgttgcagttgatcagtcaaa
agccttaacggctctaaacattgctcaagctatttcaaagcttaacggaaaaggcatggt
gaccagctcagaaattattatcgccgactacttctatcagcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGGAAAATGGCTCGTCCTTGTCA 452 481 759 Antisense
GTCCTTGTCATGCACAGAAATCCTC 375 477 774 Antisense
GAGGATACAAGAGCTTCGACACCAA 538 403 875 Antisense
AATTCCACAGGAACTCGCTTCTGTT 381 177 978 Antisense
CTCGCTTCTGTTTCTCTAATGATGC 577 233 991 Antisense
GAAATCGGTTCTGTTCACAGCTTTT 249 363 1039 Antisense
TCACAGCTTTTACATTGCGTCCGTA 403 627 1053 Antisense
GCCTTAACGGCTCTAAACATTGCTC 257 269 1120 Antisense
GAAAAGGCATGGTGACCAGCTCAGA 46 355 1166 Antisense
CCAGCTCAGAAATTATTATCGCCGA 487 273 1181 Antisense
TTATCGCCGACTACTTCTATCAGCG 150 681 1196 Antisense
Sequence Source Affymetrix Proprietary Database