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[181334_s_at]

Description C27H5.4 /REP_DB=WormBase Gene ID /WP=CE27100 /TR=Q18267 /GB=AAC46658.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 86      6grp_total > 87      4grp_total > 09      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1704
1 2 3 4 range
A 180.5
(-3)
A 		340.3
(-2.4)
P 		504.2
(-1.9)
P 		1884.4
(3)
P 		1704
B 207.1
(-3)
A 		328.9
(-2.5)
P 		385
(-2.2)
P 		1615.7
(3)
P 		1409
C 190.2
(-3)
M 		349.9
(-2.3)
P 		443.9
(-1.8)
P 		1512.6
(3)
P 		1322
avr 192.6
(-3) 		339.7
(-2.4) 		444.4
(-2) 		1670.9
(3) 		1478

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 540
1 2 3 4 5 6 range
A 189
(-3)
A 		215.5
(-2.5)
P 		265.5
(-1.5)
P 		252.2
(-1.8)
P 		412.3
(1.3)
P 		501
(3)
P 		312
B 219
(-3)
P 		275.2
(-1.9)
P 		335.2
(-0.7)
P 		289.5
(-1.6)
P 		354.8
(-0.4)
P 		528.7
(3)
P 		310
C 109
(-3)
A 		270
(-1.2)
P 		278
(-1.1)
P 		316.1
(-0.7)
P 		332.6
(-0.5)
P 		648.8
(3)
P 		540
avr 172.3
(-3) 		253.6
(-1.7) 		292.9
(-1.1) 		285.9
(-1.2) 		366.6
(0) 		559.5
(3) 		387
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 281
1 2 3 4 range
A 65.7
(-3)
A 		73.9
(-2.8)
P 		131.8
(-1.1)
P 		275.8
(3)
P 		210
B 66.1
(-3)
A 		81.2
(-2.7)
P 		89.2
(-2.5)
P 		344.3
(3)
P 		278
C 63
(-3)
M 		100.6
(-1.8)
P 		154.7
(-0.1)
P 		255
(3)
P 		192
avr 64.9
(-3) 		85.2
(-2.5) 		125.2
(-1.4) 		291.7
(3) 		227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 182
1 2 3 4 5 6 range
A 34.8
(-3)
A 		76.6
(-0.2)
P 		57.8
(-1.5)
P 		64
(-1)
P 		124.4
(3)
P 		122
(2.8)
P 		90
B 76.2
(-3)
P 		91.6
(-2.2)
P 		81.4
(-2.7)
P 		76.7
(-3)
P 		144.2
(0.4)
P 		197.1
(3)
P 		121
C 40.4
(-3)
A 		82.4
(-1.6)
P 		70.7
(-2)
P 		63.6
(-2.2)
P 		107.6
(-0.7)
P 		217.2
(3)
P 		177
avr 50.5
(-3) 		83.5
(-1.5) 		70
(-2.1) 		68.1
(-2.2) 		125.4
(0.5) 		178.8
(3) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181334_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4127
Sequence Type Exemplar sequence
Representative Public ID C27H5.4 NCBI
Target Description C27H5.4 /REP_DB=WormBase Gene ID /WP=CE27100 /TR=Q18267 /GB=AAC46658.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026827(11), NM_001026826(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026827 NCBI Caenorhabditis elegans C27H5.4b (C27H5.4) mRNA, complete cds. 11/11 None
NM_001026826 NCBI Caenorhabditis elegans C27H5.4a (C27H5.4) mRNA, complete cds. 11/11 None
SNAP00000006113 ENSEMBL cdna:SNAP chromosome:CEL140:II:7162462:7165410:-1 11/11 None
GENEFINDER00000006120 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7162462:7165768:-1 11/11 None
C27H5.4b ENSEMBL cdna:known chromosome:CEL140:II:7162462:7165768:-1 gene:C27H5.4 11/11 None
C27H5.4a ENSEMBL cdna:known chromosome:CEL140:II:7162462:7165493:-1 gene:C27H5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7162457-7165749(-) 100.0 100.0
Public Domain and Genome References
Ensembl C27H5.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP86617 Hypothetical protein C27H5.4b [Caenorhabditis elegans] ref|NP_001021998.1| C27H5.4b [Caenorhabditis elegans] 0.0
blast AAC46658 Hypothetical protein C27H5.4a [Caenorhabditis elegans] ref|NP_001021997.1| C27H5.4a [Caenorhabditis elegans] 0.0
blast CAE67552 Hypothetical protein CBG13077 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021998.1 8 7-29,39-58,82-99,114-136,148-166,176-198,341-363,477-499
NP_001021997.1 8 7-29,44-61,86-103,118-140,152-170,180-202,345-367,481-503
C27H5.4b 8 7-29,39-58,82-99,114-136,148-166,176-198,341-363,477-499
C27H5.4a 8 7-29,44-61,86-103,118-140,152-170,180-202,345-367,481-503
SNAP00000006113 9 7-29,44-61,86-103,118-140,152-170,180-202,290-312,332-354,467-489
GENEFINDER00000006120 9 7-29,39-58,82-99,114-136,148-166,176-198,286-308,328-350,463-485
Sequence
Target Sequence 
>C. ELEGANS:181334_S_AT
cgcaatttctcgttgtgggtggcaaggattctacatagttgttgctggagaagttgttga
tactgatatgaaaacaaataatagaaaaacaccattctcaacaatcattttcactttgaa
agctcttttcaacaaaccagctgatcaactagcgcctgttttcgttctctcattattttt
agaacgaggtggatacccacaattaactgttccctgccaacaattttatgaagataatta
ttcaaattcaacaatgttcaattctacatctatggaaagcaccacgttgtctggatatcc
gacagtagcagaatgcactaattattcacaatggatgttcttggctcttgcaattttccc
tgcaatctgtgcaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCAATTTCTCGTTGTGGGTGGCAA 416 251 1095 Antisense
AACACCATTCTCAACAATCATTTTC 91 137 1182 Antisense
GCTCTTTTCAACAAACCAGCTGATC 223 299 1216 Antisense
GCTGATCAACTAGCGCCTGTTTTCG 193 293 1234 Antisense
CTGTTTTCGTTCTCTCATTATTTTT 392 239 1250 Antisense
GATACCCACAATTAACTGTTCCCTG 91 425 1286 Antisense
AACTGTTCCCTGCCAACAATTTTAT 105 143 1299 Antisense
GCACCACGTTGTCTGGATATCCGAC 376 315 1373 Antisense
GATATCCGACAGTAGCAGAATGCAC 104 427 1388 Antisense
ATGGATGTTCTTGGCTCTTGCAATT 698 51 1425 Antisense
AATTTTCCCTGCAATCTGTGCAATT 362 175 1446 Antisense
Sequence Source Affymetrix Proprietary Database