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[181312_at]

Description R01H2.3 /REP_DB=WormBase Gene ID /WP=CE00802 /TR=Q21629 /GB=AAA50645.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 88      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_total > 88      4grp_total > 14      6grp_cgc > 59      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 141.4
(1.7)
P 		107.5
(-2.8)
P 		106.2
(-3)
P 		151.2
(3)
P 		45
B 142.3
(1)
P 		157.3
(3)
P 		112.3
(-3)
P 		145.5
(1.4)
P 		45
C 180.2
(0.8)
P 		87.5
(-3)
P 		116.1
(-1.8)
P 		232.3
(3)
P 		145
avr 154.6
(1) 		117.4
(-2.5) 		111.5
(-3) 		176.3
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1980
1 2 3 4 5 6 range
A 164.3
(-2.7)
P 		79.2
(-3)
P 		88.8
(-3)
P 		164.3
(-2.7)
P 		1201.7
(0.5)
P 		2022.4
(3)
P 		1943
B 123.1
(-3)
A 		123
(-3)
P 		139.3
(-2.9)
P 		219.3
(-2.7)
P 		1232.4
(0.8)
P 		1869.6
(3)
P 		1747
C 89.8
(-3)
A 		140
(-2.8)
A 		81.1
(-3)
M 		247.4
(-2.5)
P 		953.2
(-0.4)
P 		2059.2
(3)
P 		1978
avr 125.7
(-2.9) 		114.1
(-3) 		103.1
(-3) 		210.3
(-2.7) 		1129.1
(0.3) 		1983.7
(3) 		1881
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 51.5
(3)
P 		23.3
(-2.8)
P 		27.8
(-1.8)
P 		22.1
(-3)
P 		29
B 45.4
(3)
P 		38.8
(1)
P 		26
(-3)
P 		31
(-1.5)
P 		19
C 59.6
(3)
P 		25.2
(-3)
P 		40.5
(-0.3)
P 		39.2
(-0.6)
P 		34
avr 52.2
(3) 		29.1
(-3) 		31.4
(-2.4) 		30.8
(-2.6) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 678
1 2 3 4 5 6 range
A 30.3
(-2.9)
P 		28.2
(-2.9)
P 		19.3
(-3)
P 		41.7
(-2.7)
P 		362.6
(1.4)
P 		492.4
(3)
P 		473
B 42.8
(-2.9)
A 		40.9
(-2.9)
P 		33.8
(-3)
P 		58.1
(-2.8)
P 		500.7
(1.2)
P 		697.2
(3)
P 		663
C 33.3
(-2.9)
A 		42.7
(-2.8)
A 		20.6
(-3)
M 		49.7
(-2.7)
P 		308.5
(-0.4)
P 		689.3
(3)
P 		669
avr 35.5
(-2.9) 		37.3
(-2.9) 		24.6
(-3) 		49.8
(-2.7) 		390.6
(0.6) 		626.3
(3) 		602
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181312_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6823
Sequence Type Exemplar sequence
Representative Public ID R01H2.3 NCBI
Target Description R01H2.3 /REP_DB=WormBase Gene ID /WP=CE00802 /TR=Q21629 /GB=AAA50645.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066136(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066136 NCBI Caenorhabditis elegans EGG sterile (unfertilizable) family member (egg-2) (egg-2) mRNA, complete cds. 11/11 None
SNAP00000040673 ENSEMBL cdna:SNAP chromosome:CEL140:III:7058568:7062387:-1 11/11 None
GENEFINDER00000040679 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7058568:7064770:-1 11/11 None
R01H2.3 ENSEMBL cdna:known chromosome:CEL140:III:7058568:7060506:-1 gene:R01H2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7058565-7060504(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol egg-2
Ensembl R01H2.3
Entrez Gene 187510 Entrez gene
SwissProt Q21629 EMBL-EBI
WormBase CE00802 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498537 EGG sterile (unfertilizable) family member (egg-2) [Caenorhabditis elegans] gb|AAA50645.1| Egg sterile (unfertilizable) protein 2 [Caenorhabditis elegans] 0.0
blast CAE63952 Hypothetical protein CBG08534 [Caenorhabditis briggsae] 0.0
blast AAA68376 Egg sterile (unfertilizable) protein 1 [Caenorhabditis elegans] ref|NP_498237.2| EGG sterile (unfertilizable) family member (egg-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 5.3E-11
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 6.0E-6
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 4.0E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 3.6E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 7.8E-10
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.4E-11
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.1E-5
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.0E-5
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 5.3E-11
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 6.0E-6
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 4.0E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 3.6E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 7.8E-10
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.4E-11
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.1E-5
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.0E-5
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 5.3E-11
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 6.0E-6
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 4.0E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 3.6E-9
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 7.8E-10
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.4E-11
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.1E-5
Pfam IPR002172 EMBL-EBI Low density lipoprotein-receptor, class A 2.0E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498537.1 1 48-70
R01H2.3 1 48-70
SNAP00000040673 1 398-420
GENEFINDER00000040679 4 647-669,676-693,703-722,769-791
Sequence
Target Sequence 
>C. ELEGANS:181312_AT
gagcttaattgcccgaaatgcgacccaaagcacactttcacttgcccagcagtaggagga
attcataataagtgcatcaaaagaagcaaggtctgtgatggaatcttcgattgtgaagat
ggtgctgacgagaaaaagtgcacacccgtgaaggaatgtgtagtcgagagttccattcag
ttcacctgcgataataaatgtcttgaatcgagtcgtcgatgtgatggagtttgggattgt
gaagacaagtctgacgagaagggatgtgacaagtgcccatctagatctttcaaatgtagt
gccgataaaaagtgtctcccattccacacgcgttgtaatggagtagcagaatgctctgat
ggaagcgatgaacataaatgttcttgccaagaatgccttggtactcaccacgacacctac
atgtgcagtgaatcgaacagatgcttgaaacgcggcgaagtgtgctctccatattcgatg
tgccccaatgcaacctacatcgataaggcctactgcgctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCTTAATTGCCCGAAATGCGACC 322 387 1105 Antisense
AAATGCGACCCAAAGCACACTTTCA 645 119 1120 Antisense
CACTTTCACTTGCCCAGCAGTAGGA 87 197 1137 Antisense
GTTCCATTCAGTTCACCTGCGATAA 197 441 1274 Antisense
GACAAGTGCCCATCTAGATCTTTCA 508 359 1372 Antisense
GCCGATAAAAAGTGTCTCCCATTCC 592 255 1405 Antisense
AAGAATGCCTTGGTACTCACCACGA 637 149 1493 Antisense
TCACCACGACACCTACATGTGCAGT 329 623 1509 Antisense
GCGAAGTGTGCTCTCCATATTCGAT 26 289 1559 Antisense
TATTCGATGTGCCCCAATGCAACCT 639 663 1576 Antisense
TACATCGATAAGGCCTACTGCGCTT 30 645 1600 Antisense
Sequence Source Affymetrix Proprietary Database