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[181303_at]

Description C33A12.3 /REP_DB=WormBase Gene ID /WP=CE05349 /TR=Q18357 /GB=CAA92787.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 50      4grp_high_8_8 > 41      6grp_highest_nonorm > 63      6grp_total > 90      4grp_total > 54      6grp_highest_sub > 04      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 669.9
(3)
P 		399
(-3)
P 		467.9
(-1.5)
P 		503.6
(-0.7)
P 		271
B 602.2
(3)
P 		567.2
(0.5)
P 		539.2
(-1.6)
P 		519.5
(-3)
P 		83
C 663.4
(3)
P 		588
(1.4)
P 		531.6
(0.2)
P 		385.9
(-3)
P 		278
avr 645.2
(3) 		518.1
(-1.3) 		512.9
(-1.5) 		469.7
(-3) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 589.4
(1.1)
P 		625.3
(1.9)
P 		503.8
(-0.9)
P 		673.4
(3)
P 		533.9
(-0.2)
P 		410.6
(-3)
P 		263
B 621.4
(0.4)
P 		590.2
(-0.1)
P 		615.1
(0.3)
P 		770.9
(3)
P 		597.6
(0)
P 		421.2
(-3)
P 		350
C 615.9
(-0.3)
P 		612.1
(-0.4)
P 		476.8
(-3)
P 		783.9
(3)
P 		591.2
(-0.8)
P 		485.1
(-2.8)
P 		307
avr 608.9
(0.4) 		609.2
(0.4) 		531.9
(-1.2) 		742.7
(3) 		574.2
(-0.3) 		439
(-3) 		304
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 179
1 2 3 4 range
A 243.9
(3)
P 		86.6
(-2.5)
P 		122.3
(-1.3)
P 		73.7
(-3)
P 		170
B 192
(3)
P 		140.1
(-0.8)
P 		124.9
(-2)
P 		110.7
(-3)
P 		81
C 219.6
(3)
P 		169.1
(1)
P 		185.3
(1.7)
P 		65.1
(-3)
P 		155
avr 218.5
(3) 		131.9
(-0.8) 		144.2
(-0.3) 		83.2
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 143
1 2 3 4 5 6 range
A 108.6
(-2.6)
P 		222.4
(3)
P 		109.7
(-2.5)
P 		170.8
(0.5)
P 		161.1
(-0)
P 		100
(-3)
P 		122
B 216.2
(1.3)
P 		196.4
(0)
P 		149.4
(-3)
P 		204.2
(0.5)
P 		242.8
(3)
P 		157.1
(-2.5)
P 		93
C 228.3
(3)
P 		186.8
(0.7)
P 		121.3
(-3)
P 		157.6
(-1)
P 		191.3
(0.9)
P 		162.4
(-0.7)
P 		107
avr 184.4
(1.6) 		201.9
(3) 		126.8
(-3) 		177.5
(1.1) 		198.4
(2.7) 		139.8
(-2) 		75
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181303_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8815
Sequence Type Exemplar sequence
Representative Public ID C33A12.3 NCBI
Target Description C33A12.3 /REP_DB=WormBase Gene ID /WP=CE05349 /TR=Q18357 /GB=CAA92787.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069285(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069285 NCBI Caenorhabditis elegans C33A12.3 (C33A12.3) mRNA, complete cds. 11/11 None
SNAP00000009029 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9511104:9512970:-1 11/11 None
GENEFINDER00000009036 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9511104:9513015:-1 11/11 None
C33A12.3 ENSEMBL cdna:known chromosome:CEL140:IV:9510725:9513015:-1 gene:C33A12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9511109-9513021(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C33A12.3
Entrez Gene 183139 Entrez gene
SwissProt Q18357 EMBL-EBI
WormBase CE05349 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.6628.1.S1_AT similar to C33A12.3 cfa
HU35KSUBD:RC_AA343533_AT hypothetical protein LOC132321 hs
U133_X3P:HS2.128777.1.S2_3P_X_AT hypothetical protein LOC132321 hs
U133_X3P:HS2.128777.1.S2_3P_AT hypothetical protein LOC132321 hs
U133_X3P:HS2.128777.1.S1_3P_S_AT hypothetical protein LOC132321 hs
HG-U133_PLUS_2:1552370_AT hypothetical protein LOC132321 hs
HG-U133_PLUS_2:1552372_AT hypothetical protein LOC132321 hs
HG-U133_PLUS_2:1552373_S_AT hypothetical protein LOC132321 hs
HG-U95C:59943_R_AT hypothetical protein LOC132321 hs
U133_X3P:HS2.436295.1.S1_3P_X_AT Hypothetical protein LOC132321 hs
U133_X3P:HS2.436295.1.S1_3P_AT Hypothetical protein LOC132321 hs
HG-U133_PLUS_2:1569495_AT Hypothetical protein LOC132321 hs
HG-U95D:86039_I_AT Hypothetical protein LOC132321 hs
MG-U74BV2:112456_AT DNA segment, Chr 3, ERATO Doi 751, expressed mm
MOE430B:1454225_S_AT DNA segment, Chr 3, ERATO Doi 751, expressed mm
MOUSE430_2:1454225_S_AT DNA segment, Chr 3, ERATO Doi 751, expressed mm
MOUSE430_2:1430590_AT DNA segment, Chr 3, ERATO Doi 751, expressed mm
MOE430B:1430590_AT DNA segment, Chr 3, ERATO Doi 751, expressed mm
MG-U74BV2:112457_AT DNA segment, Chr 3, ERATO Doi 751, expressed mm
MOE430B:1434631_AT DNA segment, Chr 3, ERATO Doi 751, expressed (D3Ertd751e), mRNA mm
MOUSE430_2:1434631_AT DNA segment, Chr 3, ERATO Doi 751, expressed (D3Ertd751e), mRNA mm
RG-U34C:RC_AI175445_AT similar to C33A12.3-like rn
RAE230B:1392576_AT similar to C33A12.3-like rn
RAT230_2:1392576_AT similar to C33A12.3-like rn
Protein Similarities
Method ID Description E-Value
blast CAA92787 Hypothetical protein C33A12.3 [Caenorhabditis elegans] ref|NP_501686.1| C33A12.3 [Caenorhabditis elegans] 1.0E-136
blast CAA92787 Hypothetical protein C33A12.3 [Caenorhabditis elegans] ref|NP_501686.1| C33A12.3 [Caenorhabditis elegans] 1.0E-134
blast CAA92787 Hypothetical protein C33A12.3 [Caenorhabditis elegans] ref|NP_501686.1| C33A12.3 [Caenorhabditis elegans] 1.0E-128
blast CAE72764 Hypothetical protein CBG20014 [Caenorhabditis briggsae] 1.0E-124
blast CAE72764 Hypothetical protein CBG20014 [Caenorhabditis briggsae] 1.0E-122
blast CAE72764 Hypothetical protein CBG20014 [Caenorhabditis briggsae] 1.0E-121
blast XP_420450 PREDICTED: hypothetical protein XP_420450 [Gallus gallus] 1.0E-28
Sequence
Target Sequence 
>C. ELEGANS:181303_AT
cttccacttgcacatgagcctatcaaaattgacttgaagtggcactttgagcgactcgtc
gggcgcccacataagcgagttgtcaagatttcattcgacgcaccattcttcgacgatcca
gaaccactcgatccaccaggaataacaccacagctttgggatttcgaagttattgaattc
ttcttcgcaaatgatcgtggacagtatctagaagttgagattggtccacatggacattgg
ctctgccttctttttgacggagttcgtcatactatcaacaacggcgaagaactcgaactc
gaagtacgcaacaaatgggttggaaatcgttgggtaggagaagtcgagattcctcttgca
tacttcccatccaaagttacaaaatttaacgcttatcacatccatggaatggattcggaa
cgcgtttacgccgcattgagccctgtgaccgatggaacttattcggaaccagacttccac
cgcctggaattcttccaaaaaatcaacatgcgccgagtgattccagaaggttatggagat
cgtcctttcgctgatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCCACTTGCACATGAGCCTATCA 276 219 160 Antisense
TGAAGTGGCACTTTGAGCGACTCGT 16 575 194 Antisense
GGGCGCCCACATAAGCGAGTTGTCA 700 491 220 Antisense
AAGATTTCATTCGACGCACCATTCT 671 155 244 Antisense
ATTCTTCGACGATCCAGAACCACTC 381 9 264 Antisense
CTGCCTTCTTTTTGACGGAGTTCGT 569 235 402 Antisense
GAAGTCGAGATTCCTCTTGCATACT 709 335 499 Antisense
GATTCGGAACGCGTTTACGCCGCAT 454 431 571 Antisense
ATTGAGCCCTGTGACCGATGGAACT 646 3 594 Antisense
AACATGCGCCGAGTGATTCCAGAAG 474 137 664 Antisense
TATGGAGATCGTCCTTTCGCTGATT 310 655 691 Antisense
Sequence Source Affymetrix Proprietary Database