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[181297_at]

Description F59D6.4 /REP_DB=WormBase Gene ID /WP=CE11482 /TR=O16340 /GB=AAB65879.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 24.8
(0.6)
A 		2
(-3)
A 		5.5
(-2.4)
A 		39.7
(3)
A 		38
B 3.2
(-3)
A 		14.4
(-0.3)
A 		16.6
(0.3)
A 		27.9
(3)
A 		25
C 21.9
(0.2)
A 		3.1
(-2.8)
A 		2
(-3)
A 		39.2
(3)
A 		37
avr 16.6
(-0.9) 		6.5
(-3) 		8
(-2.7) 		35.6
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 4.6
(-3)
A 		9.8
(-1.2)
A 		15.8
(0.9)
A 		19.9
(2.3)
A 		21.8
(3)
A 		15
(0.6)
A 		17
B 46.2
(1.9)
A 		33.2
(0.2)
A 		9.6
(-3)
A 		54
(3)
M 		13.1
(-2.5)
A 		14.1
(-2.4)
A 		44
C 3.7
(-2.6)
A 		36.6
(3)
M 		2.8
(-2.8)
A 		9.6
(-1.6)
A 		1.4
(-3)
A 		12.1
(-1.2)
A 		35
avr 18.2
(-0.1) 		26.5
(2.6) 		9.4
(-3) 		27.8
(3) 		12.1
(-2.1) 		13.7
(-1.6) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 9
(3)
A 		0.4
(-3)
A 		1.4
(-2.3)
A 		5.8
(0.8)
A 		9
B 1
(-3)
A 		3.6
(0.2)
A 		3.8
(0.4)
A 		5.9
(3)
A 		5
C 7.3
(3)
A 		0.9
(-2.8)
A 		0.7
(-3)
A 		6.6
(2.4)
A 		7
avr 5.8
(2.6) 		1.6
(-3) 		2
(-2.5) 		6.1
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 0.8
(-3)
A 		3.5
(-0.2)
A 		3.4
(-0.3)
A 		5
(1.3)
A 		6.6
(3)
A 		3.7
(0)
A 		6
B 16.1
(3)
A 		11
(0.8)
A 		2.3
(-3)
A 		14.3
(2.2)
M 		5.3
(-1.7)
A 		5.2
(-1.7)
A 		14
C 1.4
(-2.4)
A 		11.2
(3)
M 		0.7
(-2.8)
A 		1.9
(-2.2)
A 		0.4
(-3)
A 		4.1
(-0.9)
A 		11
avr 6.1
(0.7) 		8.6
(3) 		2.1
(-3) 		7.1
(1.6) 		4.1
(-1.2) 		4.3
(-1) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181297_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15003
Sequence Type Exemplar sequence
Representative Public ID F59D6.4 NCBI
Target Description F59D6.4 /REP_DB=WormBase Gene ID /WP=CE11482 /TR=O16340 /GB=AAB65879.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071426(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071426 NCBI Caenorhabditis elegans F59D6.4 (F59D6.4) mRNA, complete cds. 11/11 None
GENEFINDER00000018896 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3029482:3034163:-1 10/11 None
F59D6.4 ENSEMBL cdna:known chromosome:CEL140:V:3029482:3031968:-1 gene:F59D6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3029481-3031968(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F59D6.4
Entrez Gene 186614 Entrez gene
SwissProt O16340 EMBL-EBI
WormBase CE11482 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4714 transmembrane receptor protein tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5006 epidermal growth factor receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB65879 Hypothetical protein F59D6.4 [Caenorhabditis elegans] ref|NP_503827.1| F59D6.4 [Caenorhabditis elegans] 0.0
blast AAB65880 Serpentine receptor, class w protein 8 [Caenorhabditis elegans] ref|NP_503828.2| Serpentine Receptor, class W family member (srw-8) [Caenorhabditis elegans] 1.0E-165
blast CAB07718 Hypothetical protein ZC482.4 [Caenorhabditis elegans] ref|NP_499660.2| ZC482.4 [Caenorhabditis elegans] 1.0E-100
blast AAB65881 Hypothetical protein F59D6.6 [Caenorhabditis elegans] ref|NP_503829.1| F59D6.6 [Caenorhabditis elegans] 1.0E-91
blast CAB07718 Hypothetical protein ZC482.4 [Caenorhabditis elegans] ref|NP_499660.2| ZC482.4 [Caenorhabditis elegans] 1.0E-90
Protein Domains
Database ID Description E-Value
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 7.7E-16
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 3.2E-8
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 7.7E-16
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 3.2E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000018896 7 45-64,74-96,109-126,136-158,165-184,223-242,263-285
Sequence
Target Sequence 
>C. ELEGANS:181297_AT
gatgcaaagctgtgtcctgtggacactaatgcaacaaaaatattgaatccattttctgaa
aatggcgatttttatattatatgtcatttcatttcgatggccttattacccagtaattgt
gacataataattgggaatttgataatagaagccggtgatgaggtatatactacaaagctg
gagtataccaagcatttgattggcacattggtgatcagaaatactaatttggtcgaaatc
gagtttttgggactgttgagctttatttgctcccttgatgagacgctaccattgattcaa
atatattcgaacaaaaatttgaaaattgcgaattttcctgaagttgctaatattataact
cgcggaaagcgtattgccattattcaggataatcacgaagatctgttcaaatttgagcct
tgtgctctattttctccattttatgagagtacttttcagaaatatcagaccaacttgaca
tatgtcggtggagactgtgggctcgacacagcggaaattaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCAAAGCTGTGTCCTGTGGACA 264 413 742 Antisense
GTCATTTCATTTCGATGGCCTTATT 419 467 824 Antisense
TCGATGGCCTTATTACCCAGTAATT 391 603 835 Antisense
AGCATTTGATTGGCACATTGGTGAT 35 79 932 Antisense
GACTGTTGAGCTTTATTTGCTCCCT 450 375 992 Antisense
TTGCTCCCTTGATGAGACGCTACCA 416 701 1008 Antisense
AATTGCGAATTTTCCTGAAGTTGCT 576 177 1065 Antisense
GCGGAAAGCGTATTGCCATTATTCA 639 293 1103 Antisense
TGAGCCTTGTGCTCTATTTTCTCCA 49 571 1155 Antisense
ATGTCGGTGGAGACTGTGGGCTCGA 497 47 1223 Antisense
GTGGGCTCGACACAGCGGAAATTAA 606 485 1238 Antisense
Sequence Source Affymetrix Proprietary Database