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[181293_at]

Description T20B6.3 /REP_DB=WormBase Gene ID /WP=CE13778 /TR=O02049 /GB=AAB52890.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 74      4grp_total > 62      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 3.4
(-3)
A 		7.5
(-2.3)
A 		38.1
(3)
A 		4.4
(-2.8)
A 		35
B 45
(3)
A 		11.2
(-2.1)
A 		35.4
(1.5)
A 		5.4
(-3)
A 		40
C 5.6
(0.2)
A 		6.4
(2.1)
A 		4.2
(-3)
A 		6.8
(3)
A 		3
avr 18
(0.7) 		8.4
(-2.1) 		25.9
(3) 		5.5
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 50.4
(3)
A 		5
(-3)
A 		12.9
(-2)
A 		33.8
(0.8)
A 		46.1
(2.4)
A 		43.7
(2.1)
A 		45
B 9.8
(-2.1)
A 		17.9
(-0)
A 		29.8
(3)
A 		6.4
(-2.9)
A 		6.1
(-3)
A 		14.6
(-0.8)
A 		24
C 3.2
(-2.9)
A 		3.1
(-3)
A 		26.6
(1.8)
A 		32.5
(3)
A 		8.1
(-1.9)
A 		2.9
(-3)
A 		30
avr 21.1
(1.8) 		8.7
(-3) 		23.1
(2.6) 		24.2
(3) 		20.1
(1.4) 		20.4
(1.5) 		16
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 1.2
(-2.6)
A 		1.6
(-2.4)
A 		10
(3)
A 		0.6
(-3)
A 		9
B 14.4
(3)
A 		2.8
(-2.2)
A 		8.2
(0.2)
A 		1.1
(-3)
A 		13
C 1.8
(3)
A 		1.8
(3)
A 		1.5
(0.4)
A 		1.1
(-3)
A 		1
avr 5.8
(2.2) 		2.1
(-1.7) 		6.6
(3) 		0.9
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 9.3
(0.7)
A 		1.8
(-3)
A 		2.8
(-2.5)
A 		8.6
(0.4)
A 		13.9
(3)
A 		10.6
(1.4)
A 		12
B 3.4
(-1.1)
A 		6
(1.7)
A 		7.2
(3)
A 		1.7
(-3)
A 		2.5
(-2.1)
A 		5.5
(1.1)
A 		6
C 1.2
(-2.7)
A 		0.9
(-3)
A 		6.8
(3)
A 		6.5
(2.7)
A 		2.6
(-1.3)
A 		1
(-2.9)
A 		6
avr 4.6
(-0) 		2.9
(-3) 		5.6
(1.8) 		5.6
(1.8) 		6.3
(3) 		5.7
(1.9) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181293_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6658
Sequence Type Exemplar sequence
Representative Public ID T20B6.3 NCBI
Target Description T20B6.3 /REP_DB=WormBase Gene ID /WP=CE13778 /TR=O02049 /GB=AAB52890.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065236(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065236 NCBI Caenorhabditis elegans T20B6.3 (T20B6.3) mRNA, complete cds. 11/11 None
SNAP00000028917 ENSEMBL cdna:SNAP chromosome:CEL140:III:2895060:2901149:-1 11/11 None
GENEFINDER00000028920 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2899431:2900276:-1 11/11 None
T20B6.3 ENSEMBL cdna:known chromosome:CEL140:III:2899431:2900392:-1 gene:T20B6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2899430-2900392(-) 54.36 100.0
Public Domain and Genome References
Gene Title
Ensembl T20B6.3
Entrez Gene 188630 Entrez gene
SwissProt O02049 EMBL-EBI
WormBase CE13778 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52890 Hypothetical protein T20B6.3 [Caenorhabditis elegans] ref|NP_497637.1| T20B6.3 [Caenorhabditis elegans] 2.0E-33
blast AAB52890 Hypothetical protein T20B6.3 [Caenorhabditis elegans] ref|NP_497637.1| T20B6.3 [Caenorhabditis elegans] 4.0E-27
blast AAB52890 Hypothetical protein T20B6.3 [Caenorhabditis elegans] ref|NP_497637.1| T20B6.3 [Caenorhabditis elegans] 6.0E-27
blast CAE69528 Hypothetical protein CBG15737 [Caenorhabditis briggsae] 4.0E-15
blast CAE69528 Hypothetical protein CBG15737 [Caenorhabditis briggsae] 2.0E-13
Sequence
Target Sequence 
>C. ELEGANS:181293_AT
aaacccctgatcgcaaaggatcctagaactcgcggccaacgaggcccattcgcagctatg
acaggaagctacggaagagggggatatggtggaagtggtggaggagcattcggaggattc
aatggaggatatggtcaaggaggatacggcggaggtggtgactttggcggcggcatggga
ggcggtggatatggaggcggtggatacggtggtggaggcgacggaggcggcggctacggt
ggatacggtggcggaggatacggtggaatgggcggaggacctggtggatacggaatggga
ggatatggaggaggcggcggcggtggtggagacttcggaggatatggaggcggtggaatg
ggcggtggtggatatggcggaggtggtgatggaggatatggaggaggtggattcggtgga
ggcggcatgggaggatacggaggaggaatgggaggcggtggatatggcggaggtggtatg
ggaggaggaggttatggaggcggcggtgatggaggatatggaccaagcggtggatatgga
ggaggctatggaccaggaggtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACCCCTGATCGCAAAGGATCCTA 346 125 163 Antisense
GCAAAGGATCCTAGAACTCGCGGCC 34 319 175 Antisense
GGTGGAGGAGCATTCGGAGGATTCA 666 497 259 Antisense
GCGGCATGGGAGGCGGTGGATATGG 118 293 332 Antisense
TACGGTGGTGGAGGCGACGGAGGCG 547 625 367 Antisense
TGGGCGGAGGACCTGGTGGATACGG 630 541 431 Antisense
GGTGGTGGAGACTTCGGAGGATATG 605 497 484 Antisense
TGGATTCGGTGGAGGCGGCATGGGA 378 549 570 Antisense
GAGGCGGCATGGGAGGATACGGAGG 628 405 581 Antisense
GGAGGTTATGGAGGCGGCGGTGATG 526 515 649 Antisense
GAGGAGGCTATGGACCAGGAGGTGG 44 405 701 Antisense
Sequence Source Affymetrix Proprietary Database