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[181285_at]

Description T20B6.2 /REP_DB=WormBase Gene ID /WP=CE13776 /TR=O02047 /GB=AAB52891.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 03      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 14.8
(-2.2)
A 		4
(-3)
A 		4.8
(-2.9)
A 		85.5
(3)
M 		82
B 4.4
(-2.1)
A 		5.5
(-0.6)
A 		3.7
(-3)
A 		8.2
(3)
A 		5
C 5.4
(-2.9)
A 		4
(-3)
A 		4.1
(-3)
A 		84
(3)
A 		80
avr 8.2
(-2.6) 		4.5
(-3) 		4.2
(-3) 		59.2
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 4.4
(-1)
A 		2.8
(-3)
A 		3.7
(-1.9)
A 		3.6
(-2)
A 		7.5
(3)
A 		5.4
(0.3)
A 		5
B 4.2
(-2.7)
A 		6.1
(-2.3)
A 		4.4
(-2.7)
A 		3
(-3)
A 		28.6
(3)
A 		6.8
(-2.1)
A 		26
C 5.4
(-2.2)
A 		21.5
(3)
A 		4
(-2.6)
A 		6.5
(-1.8)
A 		2.8
(-3)
A 		3.1
(-2.9)
A 		19
avr 4.7
(-2.5) 		10.1
(1.1) 		4
(-3) 		4.4
(-2.7) 		13
(3) 		5.1
(-2.3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 5.4
(-0.7)
A 		0.9
(-3)
A 		1.2
(-2.8)
A 		12.5
(3)
M 		12
B 1.4
(0.8)
A 		1.4
(0.8)
A 		0.9
(-3)
A 		1.7
(3)
A 		1
C 1.8
(-2.7)
A 		1.1
(-3)
A 		1.4
(-2.9)
A 		14.2
(3)
A 		13
avr 2.9
(-1.7) 		1.1
(-3) 		1.2
(-2.9) 		9.5
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 0.8
(-3)
A 		1
(-2.2)
A 		0.8
(-3)
A 		0.9
(-2.6)
A 		2.3
(3)
A 		1.3
(-1)
A 		2
B 1.5
(-2.6)
A 		2
(-2.3)
A 		1.1
(-2.8)
A 		0.8
(-3)
A 		11.6
(3)
A 		2.6
(-2)
A 		11
C 2
(-1.8)
A 		6.6
(3)
A 		1
(-2.9)
A 		1.3
(-2.6)
A 		0.9
(-3)
A 		1
(-2.9)
A 		6
avr 1.4
(-2.4) 		3.2
(0.4) 		1
(-3) 		1
(-3) 		4.9
(3) 		1.6
(-2.1) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181285_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6454
Sequence Type Exemplar sequence
Representative Public ID T20B6.2 NCBI
Target Description T20B6.2 /REP_DB=WormBase Gene ID /WP=CE13776 /TR=O02047 /GB=AAB52891.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065234(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065234 NCBI Caenorhabditis elegans T20B6.2 (T20B6.2) mRNA, complete cds. 11/11 None
T20B6.2 ENSEMBL cdna:known chromosome:CEL140:III:2893423:2897173:1 gene:T20B6.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000028923 8/11 Cross Hyb Matching Probes None
SNAP00000028916 8/11 Cross Hyb Matching Probes None
SNAP00000028918 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2893422-2897173(+) 99.95 100.0
Public Domain and Genome References
Gene Title
Ensembl T20B6.2
Entrez Gene 188629 Entrez gene
SwissProt O02047 EMBL-EBI
WormBase CE13776 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB52891 Hypothetical protein T20B6.2 [Caenorhabditis elegans] ref|NP_497635.1| T20B6.2 [Caenorhabditis elegans] 0.0
blast AAK18987 Hypothetical protein W03F11.4 [Caenorhabditis elegans] ref|NP_490945.1| W03F11.4 [Caenorhabditis elegans] 2.0E-52
blast CAB04464 Hypothetical protein F54F12.1 [Caenorhabditis elegans] ref|NP_499709.1| F54F12.1 [Caenorhabditis elegans] 5.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 2.1E-30
Sequence
Target Sequence 
>C. ELEGANS:181285_AT
aacagtgcttctaatgatggtccagagtcatctattgcactggtgttggtggcagccgga
gtgattgtgtatatcatgatgcggaagaaaccagagccccagaacgtggaaaagcctatt
ccgaaaccgaatcctccacccgctccagccaaaccagatccagtgccagtggttgttgca
attgatccagttgtcccagcacctcctgaggtcgttccggaagaagtgaaagaacttgtc
gttgaggatacccaatcctctttgatgtctgacactcaatatagctgtagagaagacttg
gtcgcttcacttgccgatcattcagtgaaagccatacaaaaccagatggatgaaaatttc
aagttgccaaatgaatgggcaaagttgttcaatcgatgctttgtcacacttgcagagttg
aacacatcttccgttcatttgcgagccgagtacaatggcagttttggagggattcaagaa
tccatttatcaatgccaatgtgatgatgtttgggagtaccgaatggatcatggcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAGTGCTTCTAATGATGGTCCAG 236 137 1561 Antisense
TGGTCCAGAGTCATCTATTGCACTG 158 553 1578 Antisense
GGTGGCAGCCGGAGTGATTGTGTAT 464 499 1608 Antisense
GATCCAGTGCCAGTGGTTGTTGCAA 475 415 1717 Antisense
CCTCCTGAGGTCGTTCCGGAAGAAG 254 265 1762 Antisense
TACCCAATCCTCTTTGATGTCTGAC 559 649 1809 Antisense
GAGAAGACTTGGTCGCTTCACTTGC 689 389 1850 Antisense
ATCGATGCTTTGTCACACTTGCAGA 187 37 1952 Antisense
TGCAGAGTTGAACACATCTTCCGTT 303 575 1971 Antisense
TTCCGTTCATTTGCGAGCCGAGTAC 555 697 1989 Antisense
GGGAGTACCGAATGGATCATGGCGC 351 493 2072 Antisense
Sequence Source Affymetrix Proprietary Database