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[181284_at]

Description K04F1.1 /REP_DB=WormBase Gene ID /WP=CE19952 /TR=Q9TXL6 /GB=AAC78175.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 48      4grp_total > 60      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 33.8
(0.9)
A 		46.6
(2.5)
A 		51.1
(3)
A 		1.5
(-3)
A 		50
B 21.6
(-3)
A 		37
(3)
A 		30.7
(0.6)
A 		21.5
(-3)
A 		16
C 37.6
(1.5)
A 		8.9
(-3)
A 		46.8
(3)
A 		12.7
(-2.4)
A 		38
avr 31
(0.7) 		30.8
(0.7) 		42.9
(3) 		11.9
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 101.3
(3)
A 		12.8
(-3)
A 		20.8
(-2.5)
A 		55.5
(-0.1)
A 		42.9
(-1)
A 		52.9
(-0.3)
A 		89
B 11.3
(-3)
A 		17.8
(-2)
A 		36.8
(0.8)
A 		52
(3)
A 		22.5
(-1.3)
A 		29.7
(-0.3)
A 		41
C 46.2
(2.2)
A 		51.5
(3)
A 		14.8
(-2.6)
A 		12.5
(-3)
A 		36.2
(0.6)
A 		46.7
(2.3)
A 		39
avr 52.9
(3) 		27.4
(-2.3) 		24.1
(-3) 		40
(0.3) 		33.9
(-1) 		43.1
(1) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 12.3
(2.5)
A 		10.1
(1.5)
A 		13.4
(3)
A 		0.2
(-3)
A 		13
B 6.9
(0.1)
A 		9.1
(3)
A 		7.1
(0.3)
A 		4.6
(-3)
A 		5
C 12.5
(1.4)
A 		2.6
(-2.8)
A 		16.3
(3)
A 		2.1
(-3)
A 		14
avr 10.6
(2) 		7.3
(0) 		12.3
(3) 		2.3
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 18.7
(3)
A 		4.6
(-3)
A 		4.5
(-3)
A 		14.1
(1.1)
A 		12.9
(0.5)
A 		12.9
(0.5)
A 		14
B 3.9
(-3)
A 		5.9
(-1.8)
A 		8.9
(0)
A 		13.8
(3)
A 		9.1
(0.2)
A 		11.1
(1.4)
A 		10
C 17.1
(3)
A 		15.7
(2.4)
A 		3.8
(-2.5)
A 		2.5
(-3)
A 		11.7
(0.8)
A 		15.6
(2.4)
A 		15
avr 13.2
(3) 		8.7
(-0.6) 		5.7
(-3) 		10.1
(0.5) 		11.2
(1.4) 		13.2
(3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181284_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15569
Sequence Type Exemplar sequence
Representative Public ID K04F1.1 NCBI
Target Description K04F1.1 /REP_DB=WormBase Gene ID /WP=CE19952 /TR=Q9TXL6 /GB=AAC78175.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071070(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071070 NCBI Caenorhabditis elegans K04F1.1 (K04F1.1) mRNA, complete cds. 10/11 None
K04F1.1 ENSEMBL cdna:known chromosome:CEL140:V:1657903:1659465:-1 gene:K04F1.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011962 3/11 Cross Hyb Matching Probes None
GENEFINDER00000011968 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1657902-1659465(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K04F1.1
Entrez Gene 186988 Entrez gene
SwissProt Q9TXL6 EMBL-EBI
WormBase CE36877 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78175 Hypothetical protein K04F1.1 [Caenorhabditis elegans] ref|NP_503471.2| K04F1.1 [Caenorhabditis elegans] 1.0E-129
blast AAB54206 Hypothetical protein F32B5.1 [Caenorhabditis elegans] ref|NP_491027.1| F32B5.1 [Caenorhabditis elegans] 7.0E-62
blast NP_491057 W10C8.5 [Caenorhabditis elegans] gb|AAK21503.1| Hypothetical protein W10C8.5 [Caenorhabditis elegans] 5.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR000749 EMBL-EBI ATP:guanido phosphotransferase 1.5E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503471.2 1 39-58
K04F1.1 1 39-58
Sequence
Target Sequence 
>C. ELEGANS:181284_AT
cgtctacactcactacacaggtaattcaaaagccgggcaaagcctattttcagaaagaaa
aatattttacaaatctctaaatataattttccagcaacttcaagccaaatccgaagtcga
cgccgccacaatcaaaaagatcgaggaggcctacgtcaagcttaatggtccagaaggagc
caagtgcaagtcgctgctcaggaagtatttgaccaaggacattgtcgagaagctcaagac
aaagtgcacaaagctcggagcatcagtgtacgactgcattttgacaggcctacgctttgt
tcgacaaggcctacgctttgtcgcattgttcaacaagaacattgaggatttttatggatt
cagtccaaaggagaagcaaccaccagttgatcttggcgagggaaagactaaggaattccc
gccattggatccgaatggaaagtacatcaagttaagggttccacgcggtcaggagaacta
tcttgagatggaaggaaaggtgaagagggatttctctgaatattctcacaaggagctcaa
gcgcaaatactatcccttgtatgtgcgcggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTCTACACTCACTACACAGGTAAT 24 247 168 Antisense
AAAGCCGGGCAAAGCCTATTTTCAG 305 123 196 Antisense
GAGGCCTACGTCAAGCTTAATGGTC 271 403 313 Antisense
AGCTCGGAGCATCAGTGTACGACTG 271 81 419 Antisense
GACTGCATTTTGACAGGCCTACGCT 506 375 439 Antisense
TACGCTTTGTTCGACAAGGCCTACG 524 649 458 Antisense
CTACGCTTTGTCGCATTGTTCAACA 620 225 478 Antisense
AATTCCCGCCATTGGATCCGAATGG 20 177 581 Antisense
CAAGTTAAGGGTTCCACGCGGTCAG 242 183 615 Antisense
GAGCTCAAGCGCAAATACTATCCCT 31 389 700 Antisense
TACTATCCCTTGTATGTGCGCGGAA 656 643 715 Antisense
Sequence Source Affymetrix Proprietary Database