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[181266_at]

Description T25B2.1 /REP_DB=WormBase Gene ID /WP=CE14170 /TR=O61752 /GB=AAC16999.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 16      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 6.7
(-3)
A 		13.2
(-2.2)
A 		13.8
(-2.1)
A 		52.9
(3)
A 		46
B 3.3
(-3)
A 		16.3
(-1.2)
A 		47.5
(3)
A 		15.8
(-1.3)
A 		44
C 11.9
(-2.8)
A 		24.9
(-2)
A 		8.8
(-3)
A 		102.1
(3)
A 		93
avr 7.3
(-3) 		18.1
(-1.7) 		23.4
(-1.1) 		56.9
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 5.1
(-3)
A 		60.2
(3)
A 		59.9
(3)
A 		37.9
(0.6)
A 		10.4
(-2.4)
A 		45
(1.3)
A 		55
B 38.4
(1.6)
A 		46
(3)
A 		12.3
(-3)
A 		13.6
(-2.8)
A 		39.3
(1.8)
A 		31.1
(0.3)
A 		34
C 50.6
(0.5)
A 		82
(3)
A 		6.2
(-3)
A 		58.1
(1.1)
A 		48
(0.3)
A 		5.7
(-3)
A 		76
avr 31.4
(-2.1) 		62.7
(3) 		26.1
(-3) 		36.5
(-1.3) 		32.6
(-1.9) 		27.3
(-2.8) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.4
(-3)
A 		2.9
(-2.4)
A 		3.6
(-1.6)
A 		7.7
(3)
A 		5
B 1.1
(-3)
A 		4
(-1.2)
A 		11
(3)
A 		3.4
(-1.6)
A 		10
C 3.9
(-2.7)
A 		7.1
(-1.3)
A 		3.1
(-3)
A 		17.2
(3)
A 		14
avr 2.5
(-3) 		4.7
(-1.1) 		5.9
(-0) 		9.4
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 0.9
(-3)
A 		21.4
(3)
A 		13
(0.5)
A 		9.6
(-0.5)
A 		3.1
(-2.4)
A 		11
(-0)
A 		21
B 13.3
(1.8)
A 		15.3
(2.7)
A 		3
(-3)
A 		3.6
(-2.7)
A 		16
(3)
A 		11.6
(1)
A 		13
C 18.7
(1.4)
A 		25
(3)
A 		1.6
(-3)
A 		11.7
(-0.4)
A 		15.5
(0.6)
A 		1.9
(-2.9)
A 		23
avr 11
(-0.9) 		20.6
(3) 		5.9
(-3) 		8.3
(-2) 		11.5
(-0.7) 		8.2
(-2.1) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181266_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16749
Sequence Type Exemplar sequence
Representative Public ID T25B2.1 NCBI
Target Description T25B2.1 /REP_DB=WormBase Gene ID /WP=CE14170 /TR=O61752 /GB=AAC16999.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076633(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076633 NCBI Caenorhabditis elegans T25B2.1 (T25B2.1) mRNA, complete cds. 11/11 None
T25B2.1 ENSEMBL cdna:known chromosome:CEL140:X:6174713:6177027:1 gene:T25B2.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000005634 6/11 Cross Hyb Matching Probes None
SNAP00000005633 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6174712-6177027(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T25B2.1
Entrez Gene 188873 Entrez gene
SwissProt O61752 EMBL-EBI
WormBase CE14170 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC16999 Hypothetical protein T25B2.1 [Caenorhabditis elegans] ref|NP_509034.1| T25B2.1 [Caenorhabditis elegans] 1.0E-133
blast CAE68881 Hypothetical protein CBG14847 [Caenorhabditis briggsae] 1.0E-87
blast T16865 hypothetical protein T13C5.2 - Caenorhabditis elegans 9.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 4.1E-49
Pfam IPR005044 EMBL-EBI Caenorhabditis protein of unknown function DUF282 5.6E-8
Sequence
Target Sequence 
>C. ELEGANS:181266_AT
tgaatttgactcacgaaccatcctgccaggcccatcactagtgattgcacattgtcaaga
aacaggggctaatgctgggacttggtactatggcattccgcctctggtaactccatttat
ttttctgaaaagtattttcacagcttgtaacgcatgtcccaaagtctacgacaatacttg
ccaaggctttggaattcctaatttactacagtggtgtcctacagctgccgaagctgggat
tacatatgtactgggatttgtgaatgctcttcttccatttctaccagctggttcttgcgg
aacaactattgtctgcccgctgacaacagcgctcaggataaaaattctgggctctgagat
accagcaccagttttttatgcttggtgcgaagagtctggggcaaaagctggaatatggta
tactggaacctggcttacaccgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATTTGACTCACGAACCATCCTG 374 573 210 Antisense
GCCAGGCCCATCACTAGTGATTGCA 92 281 234 Antisense
GGACTTGGTACTATGGCATTCCGCC 621 525 287 Antisense
CGCCTCTGGTAACTCCATTTATTTT 608 255 308 Antisense
GTCTACGACAATACTTGCCAAGGCT 342 469 373 Antisense
GGATTTGTGAATGCTCTTCTTCCAT 99 511 463 Antisense
TCTACCAGCTGGTTCTTGCGGAACA 131 613 489 Antisense
GCGGAACAACTATTGTCTGCCCGCT 43 295 506 Antisense
TGCCCGCTGACAACAGCGCTCAGGA 615 583 523 Antisense
GGCTCTGAGATACCAGCACCAGTTT 162 539 559 Antisense
TACTGGAACCTGGCTTACACCGATA 405 645 630 Antisense
Sequence Source Affymetrix Proprietary Database