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[181265_at]

Description R12E2.2 /REP_DB=WormBase Gene ID /WP=CE18134 /TR=O61794 /GB=AAC17023.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 77      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 93      6grp_total > 78      4grp_total > 04      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1189
1 2 3 4 range
A 607
(-0.4)
P 		330.5
(-3)
P 		355.8
(-2.8)
P 		957
(3)
P 		627
B 596.4
(-1.9)
P 		479.5
(-2.5)
P 		385.5
(-3)
P 		1519
(3)
P 		1134
C 769.6
(-0.3)
P 		414.1
(-3)
P 		481.8
(-2.5)
P 		1196.7
(3)
P 		783
avr 657.7
(-1.2) 		408
(-3) 		407.7
(-3) 		1224.2
(3) 		817

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 507
1 2 3 4 5 6 range
A 266.3
(-3)
P 		445.6
(0.3)
P 		304
(-2.3)
P 		306.5
(-2.3)
P 		445.5
(0.3)
P 		596.8
(3)
P 		331
B 364.7
(-2.4)
P 		374.9
(-2.2)
P 		326.7
(-3)
P 		415.1
(-1.5)
P 		479
(-0.4)
P 		677.8
(3)
P 		351
C 318.1
(-3)
P 		388.2
(-2.1)
P 		331.1
(-2.8)
P 		333.5
(-2.8)
P 		469.9
(-1)
P 		773.5
(3)
P 		455
avr 316.4
(-3) 		402.9
(-1.6) 		320.6
(-2.9) 		351.7
(-2.4) 		464.8
(-0.6) 		682.7
(3) 		366
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 252
1 2 3 4 range
A 221
(3)
P 		71.7
(-3)
P 		93
(-2.1)
P 		140.1
(-0.3)
P 		149
B 190.2
(-0.4)
P 		118.4
(-2.3)
P 		89.3
(-3)
P 		323.7
(3)
P 		234
C 254.8
(3)
P 		119
(-3)
P 		167.9
(-0.8)
P 		201.8
(0.7)
P 		136
avr 222
(3) 		103
(-3) 		116.7
(-2.3) 		221.9
(3) 		119

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 49.1
(-3)
P 		158.5
(3)
P 		66.2
(-2.1)
P 		77.7
(-1.4)
P 		134.4
(1.7)
P 		145.3
(2.3)
P 		109
B 126.9
(-1.4)
P 		124.8
(-1.4)
P 		79.3
(-3)
P 		109.9
(-1.9)
P 		194.6
(1)
P 		252.8
(3)
P 		174
C 117.9
(-1.4)
P 		118.4
(-1.4)
P 		84.3
(-2.5)
P 		67.1
(-3)
P 		152
(-0.3)
P 		258.9
(3)
P 		192
avr 98
(-2.1) 		133.9
(-0.6) 		76.6
(-3) 		84.9
(-2.7) 		160.3
(0.5) 		219
(3) 		142
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181265_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2522
Sequence Type Exemplar sequence
Representative Public ID R12E2.2 NCBI
Target Description R12E2.2 /REP_DB=WormBase Gene ID /WP=CE18134 /TR=O61794 /GB=AAC17023.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058920(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058920 NCBI Caenorhabditis elegans R12E2.2 (R12E2.2) mRNA, complete cds. 11/11 None
GENEFINDER00000012689 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4178568:4181739:1 11/11 None
SNAP00000012686 ENSEMBL cdna:SNAP chromosome:CEL140:I:4178862:4181739:1 11/11 None
R12E2.2.2 ENSEMBL cdna:known chromosome:CEL140:I:4178811:4182114:1 gene:R12E2.2 11/11 None
R12E2.2.1 ENSEMBL cdna:known chromosome:CEL140:I:4178813:4182159:1 gene:R12E2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4178861-4181739(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12E2.2
Entrez Gene 172011 Entrez gene
SwissProt O61794 EMBL-EBI
WormBase CE28764 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491321 R12E2.2 [Caenorhabditis elegans] gb|AAC17023.2| Hypothetical protein R12E2.2 [Caenorhabditis elegans] 0.0
blast CAE66742 Hypothetical protein CBG12092 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491321.1 1 646-668
R12E2.2.2 1 646-668
R12E2.2.1 1 646-668
GENEFINDER00000012689 1 699-721
SNAP00000012686 1 646-668
Sequence
Target Sequence 
>C. ELEGANS:181265_AT
caagtccaacgatagctcggctacctctaattattcccgctttgaacagaaagcttgaaa
acttcacgaactttgaggaaagaatgaaaaagatttacgaaactgccaaatcggtgatgt
tcggttcactgacatggaatactgatcatctaattgtggctttaatatcattcaacatca
tggcactatcatttctttttgctggcgtcttctacattcatcgaagaaataaggaaagat
gcgaagaaactcaaatcattgtgaaaaacgaattgagagctcgaatcgcgaaagttggaa
tagagaatcgcaagctgatctcaaaaggaatgcgaagagcagagctggcggtcactgcgg
ctgtttcatctgcgttgaagattgaaaagacgtcgagtaatcgcagtgcgatgacagaat
tagagacggcacttgcgaatttgtttgaggctcaacagacgcggattgaggagcaatttg
agcagaatcagaaaatgctccgagacgctttgtcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGTCCAACGATAGCTCGGCTACC 611 183 1784 Antisense
CTCGGCTACCTCTAATTATTCCCGC 531 231 1799 Antisense
GGTGATGTTCGGTTCACTGACATGG 679 497 1896 Antisense
ACATCATGGCACTATCATTTCTTTT 63 107 1958 Antisense
CTGGCGTCTTCTACATTCATCGAAG 476 241 1985 Antisense
GCGAAGAGCAGAGCTGGCGGTCACT 370 287 2115 Antisense
CGGCTGTTTCATCTGCGTTGAAGAT 650 239 2141 Antisense
AGACGTCGAGTAATCGCAGTGCGAT 499 69 2171 Antisense
GACGGCACTTGCGAATTTGTTTGAG 656 373 2208 Antisense
GTTTGAGGCTCAACAGACGCGGATT 513 451 2226 Antisense
GAAAATGCTCCGAGACGCTTTGTCG 18 353 2274 Antisense
Sequence Source Affymetrix Proprietary Database