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[181262_s_at]

Description F53A9.4 /REP_DB=WormBase Gene ID /WP=CE02764 /TR=Q20687 /GB=AAC46559.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 13      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 110
1 2 3 4 range
A 35.9
(0.9)
A 		16
(-1.5)
A 		3.4
(-3)
A 		54
(3)
A 		51
B 49.4
(3)
A 		19.7
(-3)
A 		22.2
(-2.5)
A 		46.4
(2.4)
P 		30
C 52
(-0.4)
P 		6.4
(-3)
A 		25.9
(-1.9)
A 		112.9
(3)
P 		107
avr 45.8
(0.3) 		14
(-3) 		17.2
(-2.7) 		71.1
(3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 14.9
(-1.8)
A 		35
(0.6)
A 		52.6
(2.7)
A 		54.9
(3)
A 		32.4
(0.3)
A 		4.7
(-3)
A 		50
B 62.4
(3)
P 		28.1
(-0.5)
A 		4.3
(-3)
A 		37.7
(0.4)
A 		11.6
(-2.2)
A 		25.6
(-0.8)
A 		58
C 52.2
(3)
M 		11.2
(-2.1)
A 		22.4
(-0.7)
A 		35.5
(0.9)
A 		4.2
(-3)
A 		46.2
(2.3)
M 		48
avr 43.2
(3) 		24.8
(-1.1) 		26.4
(-0.7) 		42.7
(2.9) 		16.1
(-3) 		25.5
(-0.9) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 13.1
(3)
A 		3.5
(-1.7)
A 		0.9
(-3)
A 		7.9
(0.4)
A 		12
B 15.7
(3)
A 		4.9
(-3)
A 		5.2
(-2.8)
A 		9.9
(-0.2)
P 		11
C 17.2
(2.4)
P 		1.8
(-3)
A 		9
(-0.5)
A 		19
(3)
P 		17
avr 15.3
(3) 		3.4
(-3) 		5
(-2.2) 		12.3
(1.5) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 2.8
(-2.2)
A 		12.4
(2.3)
A 		11.5
(1.9)
A 		13.9
(3)
A 		9.8
(1.1)
A 		1.2
(-3)
A 		13
B 21.7
(3)
P 		9.3
(-0.6)
A 		1
(-3)
A 		10
(-0.4)
A 		4.7
(-1.9)
A 		9.5
(-0.5)
A 		21
C 19.4
(3)
M 		3.4
(-2.3)
A 		5.7
(-1.6)
A 		7.1
(-1.1)
A 		1.4
(-3)
A 		15.5
(1.7)
M 		18
avr 14.6
(3) 		8.4
(-1) 		6.1
(-2.5) 		10.3
(0.2) 		5.3
(-3) 		8.7
(-0.8) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181262_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16184
Sequence Type Exemplar sequence
Representative Public ID F53A9.4 NCBI
Target Description F53A9.4 /REP_DB=WormBase Gene ID /WP=CE02764 /TR=Q20687 /GB=AAC46559.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077070(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077070 NCBI Caenorhabditis elegans F53A9.4 (F53A9.4) mRNA, complete cds. 11/11 A 
SNAP00000001073 ENSEMBL cdna:SNAP chromosome:CEL140:X:8704247:8707068:1 11/11 None
GENEFINDER00000001085 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8704247:8707068:1 11/11 None
F53A9.4 ENSEMBL cdna:known chromosome:CEL140:X:8704247:8707135:1 gene:F53A9.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8704245-8707141(+) 99.22 100.0
Public Domain and Genome References
Gene Title
Ensembl F53A9.4
Entrez Gene 181116 Entrez gene
SwissProt Q20687 EMBL-EBI
WormBase CE30141 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46559 Hypothetical protein F53A9.4 [Caenorhabditis elegans] ref|NP_509471.2| F53A9.4 [Caenorhabditis elegans] 0.0
blast CAE69340 Hypothetical protein CBG15417 [Caenorhabditis briggsae] 1.0E-139
blast CAE69340 Hypothetical protein CBG15417 [Caenorhabditis briggsae] 1.0E-137
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509471.2 4 222-244,254-276,297-319,339-361
F53A9.4 4 222-244,254-276,297-319,339-361
SNAP00000001073 4 222-244,254-276,297-319,339-361
GENEFINDER00000001085 4 231-253,263-285,306-328,348-370
Sequence
Target Sequence 
>C. ELEGANS:181262_S_AT
ggacttatcacattcattgtatggtttgttggattggtttcaacacttttgtgcatccaa
ttggaaagtcaactcgcatttgcgccaacaagaatcccttccgaagacctagttcaccac
tggagagttttcaacgtgttgagggctctcacatcaggtcttgggtttttgttgctggca
ttcaaacctgattctgatacaatggcaaaggaattgagagagttaagcaatttacgaaca
attggaacttgtgacttcggatccagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACTTATCACATTCATTGTATGGT 222 525 913 Antisense
GGTTTCAACACTTTTGTGCATCCAA 592 505 948 Antisense
TGGAAAGTCAACTCGCATTTGCGCC 620 547 974 Antisense
GCATTTGCGCCAACAAGAATCCCTT 79 309 988 Antisense
GAATCCCTTCCGAAGACCTAGTTCA 554 319 1004 Antisense
TCCGAAGACCTAGTTCACCACTGGA 552 595 1012 Antisense
TCAACGTGTTGAGGGCTCTCACATC 261 631 1043 Antisense
CTCTCACATCAGGTCTTGGGTTTTT 325 231 1058 Antisense
TCTTGGGTTTTTGTTGCTGGCATTC 242 619 1071 Antisense
GCTGGCATTCAAACCTGATTCTGAT 59 295 1086 Antisense
GGAACTTGTGACTTCGGATCCAGAA 573 529 1156 Antisense
Sequence Source Affymetrix Proprietary Database