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[181261_at]

Description F53A9.4 /REP_DB=WormBase Gene ID /WP=CE02764 /TR=Q20687 /GB=AAC46559.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 27      4grp_total > 14      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 27.3
(-1.2)
A 		6.6
(-3)
A 		24.1
(-1.5)
A 		74.6
(3)
M 		68
B 43.9
(0.5)
A 		15.9
(-1.9)
A 		2.8
(-3)
A 		73.7
(3)
A 		71
C 53.6
(3)
A 		11.4
(-2.5)
A 		7.9
(-3)
A 		21.9
(-1.2)
A 		46
avr 41.6
(1) 		11.3
(-3) 		11.6
(-3) 		56.7
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 23.6
(-0.1)
A 		26.3
(0.3)
A 		26.1
(0.3)
M 		38.6
(2.6)
A 		7.7
(-3)
A 		41.1
(3)
A 		33
B 56.5
(3)
A 		8.1
(-3)
A 		19.9
(-1.5)
A 		7.8
(-3)
A 		27.7
(-0.5)
A 		37.8
(0.7)
A 		49
C 45.2
(3)
A 		35.5
(1.4)
A 		14.1
(-2)
A 		15.3
(-1.8)
A 		13
(-2.2)
A 		7.9
(-3)
A 		37
avr 41.8
(3) 		23.3
(-1.3) 		20
(-2.1) 		20.6
(-1.9) 		16.1
(-3) 		28.9
(-0) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 9.9
(2.4)
A 		1.4
(-3)
A 		6.3
(0.1)
A 		10.9
(3)
M 		10
B 14
(2.3)
A 		3.9
(-1.7)
A 		0.7
(-3)
A 		15.7
(3)
A 		15
C 17.7
(3)
A 		3.3
(-2.8)
A 		2.7
(-3)
A 		3.7
(-2.6)
A 		15
avr 13.9
(3) 		2.9
(-3) 		3.2
(-2.8) 		10.1
(0.9) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 4.3
(-1.4)
A 		9.4
(2.5)
A 		5.7
(-0.4)
M 		9.8
(2.8)
A 		2.3
(-3)
A 		10
(3)
A 		8
B 19.7
(3)
A 		2.7
(-2.8)
A 		4.8
(-2.1)
A 		2.1
(-3)
A 		11.3
(0.1)
A 		14.1
(1.1)
A 		18
C 16.7
(3)
A 		10.8
(0.5)
A 		3.6
(-2.6)
A 		3.1
(-2.8)
A 		4.2
(-2.3)
A 		2.6
(-3)
A 		14
avr 13.6
(3) 		7.6
(-1) 		4.7
(-3) 		5
(-2.8) 		5.9
(-2.2) 		8.9
(-0.2) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181261_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16184
Sequence Type Exemplar sequence
Representative Public ID F53A9.4 NCBI
Target Description F53A9.4 /REP_DB=WormBase Gene ID /WP=CE02764 /TR=Q20687 /GB=AAC46559.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077070(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077070 NCBI Caenorhabditis elegans F53A9.4 (F53A9.4) mRNA, complete cds. 11/11 A 
SNAP00000001073 ENSEMBL cdna:SNAP chromosome:CEL140:X:8704247:8707068:1 11/11 None
GENEFINDER00000001085 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8704247:8707068:1 11/11 None
F53A9.4 ENSEMBL cdna:known chromosome:CEL140:X:8704247:8707135:1 gene:F53A9.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8704245-8707141(+) 99.22 100.0
Public Domain and Genome References
Gene Title
Ensembl F53A9.4
Entrez Gene 181116 Entrez gene
SwissProt Q20687 EMBL-EBI
WormBase CE30141 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46559 Hypothetical protein F53A9.4 [Caenorhabditis elegans] ref|NP_509471.2| F53A9.4 [Caenorhabditis elegans] 0.0
blast CAE69340 Hypothetical protein CBG15417 [Caenorhabditis briggsae] 1.0E-139
blast CAE69340 Hypothetical protein CBG15417 [Caenorhabditis briggsae] 1.0E-137
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509471.2 4 222-244,254-276,297-319,339-361
F53A9.4 4 222-244,254-276,297-319,339-361
SNAP00000001073 4 222-244,254-276,297-319,339-361
GENEFINDER00000001085 4 231-253,263-285,306-328,348-370
Sequence
Target Sequence 
>C. ELEGANS:181261_AT
atcagatcgcaaacattatacaagggtttcttgctggaatttccgttatgctggctattt
tctcatttaatttggaaccagaggttttactaactgggtatcgttacatgagccttccaa
tccatgctggtttcatggtggctttcactgttggtttggtatctgcaatagacagaactg
gaatttacgaagtggaacatttcacttccagaacacgtctcaccgcaacagtttacaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAGATCGCAAACATTATACAAGG 379 29 671 Antisense
CAAGGGTTTCTTGCTGGAATTTCCG 14 157 691 Antisense
GGAATTTCCGTTATGCTGGCTATTT 679 533 706 Antisense
GCTGGCTATTTTCTCATTTAATTTG 418 293 720 Antisense
CAGAGGTTTTACTAACTGGGTATCG 131 187 749 Antisense
CTGGGTATCGTTACATGAGCCTTCC 61 241 764 Antisense
TACATGAGCCTTCCAATCCATGCTG 587 643 775 Antisense
ATGCTGGTTTCATGGTGGCTTTCAC 222 43 794 Antisense
GTGGCTTTCACTGTTGGTTTGGTAT 412 491 808 Antisense
ACATTTCACTTCCAGAACACGTCTC 301 105 867 Antisense
ACGTCTCACCGCAACAGTTTACAAT 365 93 885 Antisense
Sequence Source Affymetrix Proprietary Database