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[181256_at]

Description F52C6.4 /REP_DB=WormBase Gene ID /WP=CE17096 /TR=O44819 /GB=AAB94982.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 19      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 72.2
(-3)
A 		74.4
(-2.7)
A 		84.7
(-1.1)
A 		111.4
(3)
P 		39
B 58.5
(-0.4)
A 		70.6
(3)
A 		49.1
(-3)
A 		54.1
(-1.6)
M 		22
C 82.2
(1.6)
A 		79.4
(1.1)
A 		58
(-3)
A 		89.6
(3)
A 		32
avr 71
(-1) 		74.8
(0.1) 		63.9
(-3) 		85
(3) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 69.8
(2.4)
A 		51.9
(-3)
A 		54.3
(-2.3)
A 		57.4
(-1.3)
A 		70
(2.5)
A 		71.7
(3)
A 		20
B 55.5
(-0.4)
A 		57.9
(0.1)
A 		44.9
(-2.3)
A 		41
(-3)
A 		73.9
(3)
M 		44.7
(-2.3)
P 		33
C 42.2
(-2.5)
A 		38.3
(-3)
M 		56
(-0.7)
A 		83.7
(3)
A 		75
(1.9)
A 		74.3
(1.8)
P 		45
avr 55.8
(-1.4) 		49.4
(-3) 		51.7
(-2.4) 		60.7
(-0.1) 		73
(3) 		63.6
(0.6) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 26.3
(3)
A 		16.1
(-3)
A 		22.1
(0.5)
A 		16.3
(-2.9)
P 		10
B 18.7
(3)
A 		17.4
(1.9)
A 		11.4
(-3)
A 		11.5
(-2.9)
M 		7
C 27.2
(3)
A 		22.8
(0.8)
A 		20.2
(-0.5)
A 		15.1
(-3)
A 		12
avr 24.1
(3) 		18.8
(-0.2) 		17.9
(-0.8) 		14.3
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 12.9
(-2.3)
A 		18.4
(1.3)
A 		11.8
(-3)
A 		14.5
(-1.3)
A 		21.1
(3)
A 		17.4
(0.6)
A 		9
B 19.3
(-0.4)
A 		19.3
(-0.4)
A 		10.9
(-3)
A 		10.9
(-3)
A 		30
(3)
M 		16.7
(-1.2)
P 		19
C 15.6
(-1.2)
A 		11.7
(-3)
M 		14.2
(-1.9)
A 		16.8
(-0.7)
A 		24.3
(2.7)
A 		24.9
(3)
P 		13
avr 15.9
(-1.3) 		16.5
(-1) 		12.3
(-3) 		14.1
(-2.2) 		25.1
(3) 		19.7
(0.5) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181256_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4453
Sequence Type Exemplar sequence
Representative Public ID F52C6.4 NCBI
Target Description F52C6.4 /REP_DB=WormBase Gene ID /WP=CE17096 /TR=O44819 /GB=AAB94982.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061725(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061725 NCBI Caenorhabditis elegans F52C6.4 (F52C6.4) mRNA, complete cds. 11/11 None
SNAP00000005724 ENSEMBL cdna:SNAP chromosome:CEL140:II:1923149:1923926:1 9/11 None
F52C6.4 ENSEMBL cdna:known chromosome:CEL140:II:1922868:1924259:1 gene:F52C6.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000005740 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:1922866-1924258(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F52C6.4
Entrez Gene 186086 Entrez gene
SwissProt O44819 EMBL-EBI
WormBase CE17096 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94982 Hypothetical protein F52C6.4 [Caenorhabditis elegans] ref|NP_494126.1| F52C6.4 [Caenorhabditis elegans] 1.0E-164
blast AAB94982 Hypothetical protein F52C6.4 [Caenorhabditis elegans] ref|NP_494126.1| F52C6.4 [Caenorhabditis elegans] 1.0E-89
blast AAB94972 Hypothetical protein F52C6.3 [Caenorhabditis elegans] ref|NP_494127.2| F52C6.3 [Caenorhabditis elegans] 1.0E-34
blast AAC13691 poly-ubiquitin [Magnaporthe grisea] 2.0E-31
blast BAB71316.1 unnamed protein product [Homo sapiens] 7.0E-29
blast AAH69831 Im:6892314 protein [Danio rerio] gb|AAH93444.1| Im:6892314 protein [Danio rerio] 4.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR000626 EMBL-EBI Ubiquitin 0.0079
Pfam IPR000626 EMBL-EBI Ubiquitin 7.7E-4
Pfam IPR000626 EMBL-EBI Ubiquitin 1.9E-30
Pfam IPR000626 EMBL-EBI Ubiquitin 7.7E-4
Pfam IPR000626 EMBL-EBI Ubiquitin 1.9E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494126.1 1 17-39
F52C6.4 1 17-39
Sequence
Target Sequence 
>C. ELEGANS:181256_AT
tcgttttcacagtctcttacggagaagtaaccggagatccaatcactgttgaagcatcgg
acgccatcaaggatcttaatttcaaggttgccgaggctcagcgaaaaccgcatcaacctt
ccaacattgttttgtgcaaggatcaacgtactatttctgactacaacatcaaaaacggca
gtactctccgtttggaagttcctgtaaaagaaaaactgacggtttctgtgaaaaccttat
tcggaaagacgattcctatttcaatcaagaatacggatactgttgagacattgaagttca
aggttcagaataaacacggtgttccaccagatcagcagcgtctcattttcggtcgggcac
agctcgagaatgaccgtgctctgttcgcctataacattcgtgatgaggatattatccatt
tagtctccagacatcggtttaaattcttctgtgttctgaatttccgact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGTTTTCACAGTCTCTTACGGAGA 185 605 278 Antisense
AGCATCGGACGCCATCAAGGATCTT 600 75 330 Antisense
TTGCCGAGGCTCAGCGAAAACCGCA 411 697 365 Antisense
GCATCAACCTTCCAACATTGTTTTG 280 309 387 Antisense
GTACTCTCCGTTTGGAAGTTCCTGT 477 459 458 Antisense
AAACACGGTGTTCCACCAGATCAGC 413 129 589 Antisense
TTTCGGTCGGGCACAGCTCGAGAAT 268 669 624 Antisense
CAGCTCGAGAATGACCGTGCTCTGT 706 203 637 Antisense
GCTCTGTTCGCCTATAACATTCGTG 363 299 655 Antisense
GATATTATCCATTTAGTCTCCAGAC 394 427 685 Antisense
TCTTCTGTGTTCTGAATTTCCGACT 603 617 722 Antisense
Sequence Source Affymetrix Proprietary Database