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[181253_at]

Description T09B4.3 /REP_DB=WormBase Gene ID /WP=CE13461 /TR=O02160 /GB=AAB53006.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 91      4grp_total > 11      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 17.4
(-2.3)
A 		7.1
(-3)
A 		15.6
(-2.5)
A 		100.7
(3)
A 		94
B 7
(-2.9)
A 		7.9
(-2.5)
A 		6.7
(-3)
A 		20.6
(3)
A 		14
C 49
(0.7)
A 		61.1
(3)
A 		40.9
(-0.8)
A 		29
(-3)
A 		32
avr 24.5
(-2.3) 		25.4
(-2.1) 		21.1
(-3) 		50.1
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 67.9
(3)
A 		21.9
(-3)
A 		51.3
(0.8)
A 		35.2
(-1.3)
A 		22.9
(-2.9)
A 		43
(-0.2)
M 		46
B 48.5
(-0.9)
A 		62.2
(1)
P 		41.4
(-2)
A 		70.5
(2.2)
A 		76.2
(3)
P 		34.1
(-3)
P 		42
C 16.4
(-3)
A 		54.7
(2.7)
M 		27.7
(-1.3)
P 		56.9
(3)
A 		49.4
(1.9)
P 		32.8
(-0.6)
A 		41
avr 44.3
(-0.4) 		46.3
(0.3) 		40.1
(-1.8) 		54.2
(3) 		49.5
(1.4) 		36.6
(-3) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 6.3
(-0.8)
A 		1.6
(-3)
A 		4.1
(-1.9)
A 		14.7
(3)
A 		13
B 2.2
(-1.6)
A 		1.9
(-2.2)
A 		1.5
(-3)
A 		4.4
(3)
A 		3
C 16.2
(2.3)
A 		17.6
(3)
A 		14.3
(1.4)
A 		4.9
(-3)
A 		13
avr 8.2
(3) 		7
(-1.5) 		6.6
(-3) 		8
(2.3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 12.5
(3)
A 		7.8
(-2)
A 		11.2
(1.6)
A 		8.9
(-0.9)
A 		6.9
(-3)
A 		10.5
(0.9)
M 		6
B 16.9
(-1)
A 		20.7
(0)
P 		10.1
(-3)
A 		18.7
(-0.5)
A 		31
(3)
P 		12.7
(-2.3)
P 		21
C 6.1
(-3)
A 		16.7
(3)
M 		7.1
(-2.4)
P 		11.4
(0)
A 		16
(2.6)
P 		11
(-0.2)
A 		11
avr 11.8
(-1.4) 		15.1
(1) 		9.5
(-3) 		13
(-0.5) 		18
(3) 		11.4
(-1.7) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181253_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2532
Sequence Type Exemplar sequence
Representative Public ID T09B4.3 NCBI
Target Description T09B4.3 /REP_DB=WormBase Gene ID /WP=CE13461 /TR=O02160 /GB=AAB53006.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059378(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059378 NCBI Caenorhabditis elegans T09B4.3 (T09B4.3) mRNA, complete cds. 11/11 None
GENEFINDER00000036049 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6184515:6186144:1 11/11 None
T09B4.3 ENSEMBL cdna:known chromosome:CEL140:I:6184515:6185925:1 gene:T09B4.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000036040 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6184443-6185854(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T09B4.3
Entrez Gene 188315 Entrez gene
SwissProt O02160 EMBL-EBI
WormBase CE13461 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB53006 Hypothetical protein T09B4.3 [Caenorhabditis elegans] ref|NP_491779.1| T09B4.3 [Caenorhabditis elegans] 6.0E-52
blast AAB53006 Hypothetical protein T09B4.3 [Caenorhabditis elegans] ref|NP_491779.1| T09B4.3 [Caenorhabditis elegans] 1.0E-46
blast CAE67127 Hypothetical protein CBG12549 [Caenorhabditis briggsae] 3.0E-32
blast CAE67127 Hypothetical protein CBG12549 [Caenorhabditis briggsae] 4.0E-17
Sequence
Target Sequence 
>C. ELEGANS:181253_AT
gaaacatgtgaagaaagcccgccggaatgtgaatctatcatttttgaagctggatctcca
attgaccgatccgaatcgttggatcagttgaaagagtcctacgaaagtttgatgaaatcg
gaaaatgaaaaaagaaagaaagatggatgttttattggattcttgacaaaagctctggct
gaagaggaaactactgaacgtggacaatttttcacgtattacctaattgatgggatctct
ctgaagccaccaaaagattcaaacatttcattggacctttacatctgttatttgaatagc
cgccgtcatcatgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACATGTGAAGAAAGCCCGCCGG 496 353 46 Antisense
AAAGCCCGCCGGAATGTGAATCTAT 559 123 59 Antisense
ATCATTTTTGAAGCTGGATCTCCAA 673 29 82 Antisense
ATCTCCAATTGACCGATCCGAATCG 5 33 99 Antisense
GGATTCTTGACAAAAGCTCTGGCTG 344 509 202 Antisense
GAGGAAACTACTGAACGTGGACAAT 543 403 229 Antisense
GTGGACAATTTTTCACGTATTACCT 391 491 245 Antisense
TGATGGGATCTCTCTGAAGCCACCA 279 567 273 Antisense
GATTCAAACATTTCATTGGACCTTT 256 431 301 Antisense
TCATTGGACCTTTACATCTGTTATT 412 617 313 Antisense
TTTGAATAGCCGCCGTCATCATGAG 355 665 336 Antisense
Sequence Source Affymetrix Proprietary Database