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[181249_at]

Description T04B8.3 /REP_DB=WormBase Gene ID /WP=CE18191 /TR=O44847 /GB=AAB95009.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 68      4grp_total > 19      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 36.1
(0.9)
A 		50.5
(3)
A 		10
(-3)
A 		42
(1.7)
A 		41
B 19.2
(-3)
A 		30.9
(-2)
A 		23.6
(-2.6)
A 		87.9
(3)
A 		69
C 36.8
(-1.4)
A 		36.5
(-1.5)
A 		19.2
(-3)
A 		86.8
(3)
A 		68
avr 30.7
(-1.6) 		39.3
(-0.6) 		17.6
(-3) 		72.2
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 249
1 2 3 4 5 6 range
A 85.7
(-0.9)
A 		15
(-3)
A 		19.9
(-2.9)
A 		69.7
(-1.4)
A 		62.5
(-1.6)
A 		219.2
(3)
P 		204
B 64.3
(-1.9)
A 		55.4
(-2.2)
A 		37.1
(-2.6)
A 		23.2
(-3)
A 		59
(-2.1)
A 		250.6
(3)
P 		227
C 52.8
(-2.4)
A 		29.3
(-3)
A 		46.4
(-2.6)
A 		36.3
(-2.8)
A 		68.6
(-2)
A 		264.1
(3)
P 		235
avr 67.6
(-2) 		33.2
(-3) 		34.5
(-3) 		43.1
(-2.7) 		63.4
(-2.1) 		244.6
(3) 		211
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 13.2
(3)
A 		11
(1.8)
A 		2.6
(-3)
A 		6.1
(-1)
A 		11
B 6.1
(-2.7)
A 		7.6
(-2)
A 		5.5
(-3)
A 		18.7
(3)
A 		13
C 12.2
(1.2)
A 		10.5
(-0.1)
A 		6.7
(-3)
A 		14.6
(3)
A 		8
avr 10.5
(1.1) 		9.7
(0.5) 		4.9
(-3) 		13.1
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 15.8
(-1.6)
A 		5.3
(-2.9)
A 		4.3
(-3)
A 		17.7
(-1.4)
A 		18.9
(-1.2)
A 		53.4
(3)
P 		49
B 22.4
(-1.9)
A 		18.4
(-2.2)
A 		9
(-2.8)
A 		6.1
(-3)
A 		24
(-1.8)
A 		93.4
(3)
P 		87
C 19.6
(-2.1)
A 		8.9
(-2.9)
A 		11.8
(-2.7)
A 		7.3
(-3)
A 		22.2
(-1.9)
A 		88.4
(3)
P 		81
avr 19.3
(-2.1) 		10.9
(-2.8) 		8.4
(-3) 		10.4
(-2.8) 		21.7
(-1.9) 		78.4
(3) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181249_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4402
Sequence Type Exemplar sequence
Representative Public ID T04B8.3 NCBI
Target Description T04B8.3 /REP_DB=WormBase Gene ID /WP=CE18191 /TR=O44847 /GB=AAB95009.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061373(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061373 NCBI Caenorhabditis elegans T04B8.3 (T04B8.3) mRNA, complete cds. 11/11 None
SNAP00000021878 ENSEMBL cdna:SNAP chromosome:CEL140:II:627520:628516:1 11/11 None
GENEFINDER00000021883 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:627520:628516:1 11/11 None
T04B8.3 ENSEMBL cdna:known chromosome:CEL140:II:625052:628668:1 gene:T04B8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:625051-628516(+) 94.34 100.0
Public Domain and Genome References
Gene Title
Ensembl T04B8.3
Entrez Gene 173454 Entrez gene
WormBase CE18191 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493774 T04B8.3 [Caenorhabditis elegans] 0.0
blast AAB95009 Hypothetical protein T04B8.3 [Caenorhabditis elegans] 1.0E-170
blast NP_493774 T04B8.3 [Caenorhabditis elegans] 1.0E-170
blast CAA90142 Hypothetical protein ZK938.2 [Caenorhabditis elegans] ref|NP_496120.1| ZK938.2 [Caenorhabditis elegans] 8.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR000698 EMBL-EBI Arrestin 4.5E-5
Pfam IPR000698 EMBL-EBI Arrestin 9.6E-20
Pfam IPR000698 EMBL-EBI Arrestin 4.5E-5
Pfam IPR000698 EMBL-EBI Arrestin 9.6E-20
Sequence
Target Sequence 
>C. ELEGANS:181249_AT
catggtcgcgacctttgttcattgaaaaagaattcaagatggttcacaagctggccgatc
agaaaatgagtcaagttatgacttatacaagtagtccaagatttgatgcattgttttcaa
acggacagccgacatttaaggttacaatgcccaagttcaccttatatcctggcgagacca
ccgaattcaaattggaagtggagaatcatagctcatcaactgtcaaaaaaatctatgccg
agctcgaagcagaaccttttcccctttatcacatatatggcaacacaaaggctaagggga
aaatgcacgttttagtagctccatactcaactcaaaagttcaccataccgtttacaatcc
caacagatttgacagcaagcttccacacttgcggatcgtccttgaagtacaccatgaaat
tcggattggtaactgactcctggctcacgacagacccggcgctagctctcgtagtttcta
ttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATGGTCGCGACCTTTGTTCATTGA 491 209 590 Antisense
AAGATGGTTCACAAGCTGGCCGATC 672 153 625 Antisense
GCCCAAGTTCACCTTATATCCTGGC 494 287 738 Antisense
TATCCTGGCGAGACCACCGAATTCA 464 657 754 Antisense
GCCGAGCTCGAAGCAGAACCTTTTC 317 283 826 Antisense
TTTTCCCCTTTATCACATATATGGC 329 677 846 Antisense
ACCATACCGTTTACAATCCCAACAG 516 81 931 Antisense
GACAGCAAGCTTCCACACTTGCGGA 353 369 960 Antisense
TGCGGATCGTCCTTGAAGTACACCA 209 583 979 Antisense
CGGATTGGTAACTGACTCCTGGCTC 308 233 1011 Antisense
CGCTAGCTCTCGTAGTTTCTATTGG 247 255 1049 Antisense
Sequence Source Affymetrix Proprietary Database