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[181239_s_at]

Description C01B4.8 /REP_DB=WormBase Gene ID /WP=CE20477 /TR=Q9UAT4 /GB=AAD14700.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_high_8_8 > 32      6grp_highest_nonorm > 80      6grp_total > 05      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 311.5
(3)
P 		125.9
(-2.2)
P 		97.9
(-3)
P 		107.1
(-2.7)
A 		214
B 304.3
(3)
P 		132
(-2.4)
P 		113.5
(-3)
P 		138
(-2.2)
P 		191
C 306
(3)
P 		139.2
(-2.3)
P 		119.8
(-2.9)
P 		116.5
(-3)
P 		190
avr 307.3
(3) 		132.4
(-2.3) 		110.4
(-3) 		120.5
(-2.7) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 361.1
(3)
P 		268.6
(0.8)
P 		160.4
(-1.8)
P 		129.6
(-2.6)
P 		135.6
(-2.4)
P 		111
(-3)
P 		250
B 422.7
(3)
P 		294.5
(0.7)
P 		107
(-2.7)
P 		141.6
(-2.1)
P 		188.9
(-1.2)
P 		91.9
(-3)
P 		331
C 333.6
(3)
P 		331.4
(2.9)
P 		151.7
(-1.6)
P 		182.2
(-0.9)
P 		160.1
(-1.4)
P 		98.2
(-3)
P 		235
avr 372.5
(3) 		298.2
(1.4) 		139.7
(-2.1) 		151.1
(-1.9) 		161.5
(-1.7) 		100.4
(-3) 		272
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 113.4
(3)
P 		27.3
(-2.3)
P 		25.6
(-2.4)
P 		15.7
(-3)
A 		98
B 97
(3)
P 		32.6
(-2.5)
P 		26.3
(-3)
P 		29.4
(-2.7)
P 		71
C 101.3
(3)
P 		40
(-1.5)
P 		41.8
(-1.4)
P 		19.7
(-3)
P 		82
avr 103.9
(3) 		33.3
(-2.1) 		31.2
(-2.3) 		21.6
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 66.6
(0.5)
P 		95.5
(3)
P 		34.9
(-2.3)
P 		32.9
(-2.5)
P 		40.9
(-1.8)
P 		27
(-3)
P 		69
B 147.1
(3)
P 		98
(0.6)
P 		26
(-3)
P 		37.5
(-2.4)
P 		76.7
(-0.5)
P 		34.3
(-2.6)
P 		121
C 123.7
(3)
P 		101.1
(1.5)
P 		38.6
(-2.6)
P 		36.6
(-2.8)
P 		51.8
(-1.8)
P 		32.9
(-3)
P 		91
avr 112.5
(3) 		98.2
(1.9) 		33.2
(-2.9) 		35.7
(-2.7) 		56.5
(-1.1) 		31.4
(-3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181239_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15940
Sequence Type Exemplar sequence
Representative Public ID C01B4.8 NCBI
Target Description C01B4.8 /REP_DB=WormBase Gene ID /WP=CE20477 /TR=Q9UAT4 /GB=AAD14700.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071255(11), NM_071281(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071255 NCBI Caenorhabditis elegans Y19D10A.5 (Y19D10A.5) mRNA, complete cds. 11/11 None
NM_071281 NCBI Caenorhabditis elegans C01B4.8 (C01B4.8) mRNA, complete cds. 11/11 None
SNAP00000008430 ENSEMBL cdna:SNAP chromosome:CEL140:V:2490340:2493448:1 11/11 None
GENEFINDER00000008435 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2490340:2492529:1 11/11 None
SNAP00000029971 ENSEMBL cdna:SNAP chromosome:CEL140:V:2382061:2385169:1 11/11 None
GENEFINDER00000029976 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2382061:2384250:1 11/11 None
Y19D10A.5 ENSEMBL cdna:known chromosome:CEL140:V:2382994:2384250:1 gene:Y19D10A.5 11/11 None
C01B4.8 ENSEMBL cdna:known chromosome:CEL140:V:2490340:2492529:1 gene:C01B4.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2490339-2492529(+) 100.0 100.0
chrV:2382060-2384250(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl Y19D10A.5
C01B4.8
Entrez Gene 182055 Entrez gene
189483 Entrez gene
SwissProt Q9UAT4 EMBL-EBI
Q966D5 EMBL-EBI
WormBase CE20477 Wormbase
CE21449 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD14700 Hypothetical protein C01B4.8 [Caenorhabditis elegans] ref|NP_503682.1| C01B4.8 [Caenorhabditis elegans] 0.0
blast NP_503656 Y19D10A.5 [Caenorhabditis elegans] gb|AAK68418.1| Hypothetical protein Y19D10A.5 [Caenorhabditis elegans] 0.0
blast CAE58041 Hypothetical protein CBG01117 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 1.2E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503656.1 10 21-43,53-75,84-106,116-135,177-199,209-231,244-266,270-292,305-327,337-359
NP_503682.1 12 27-49,89-108,115-137,147-169,176-198,208-227,269-291,301-323,336-358,362-384,397-419,429-451
Y19D10A.5 10 21-43,53-75,84-106,116-135,177-199,209-231,244-266,270-292,305-327,337-359
C01B4.8 12 27-49,89-108,115-137,147-169,176-198,208-227,269-291,301-323,336-358,362-384,397-419,429-451
SNAP00000008430 12 27-49,89-108,115-137,147-169,176-198,208-227,269-291,301-323,336-358,362-384,397-419,429-451
GENEFINDER00000008435 12 27-49,89-108,115-137,147-169,176-198,208-227,269-291,301-323,336-358,362-384,397-419,429-451
SNAP00000029971 12 27-49,89-108,115-137,147-169,176-198,208-227,269-291,301-323,336-358,362-384,397-419,429-451
GENEFINDER00000029976 12 27-49,89-108,115-137,147-169,176-198,208-227,269-291,301-323,336-358,362-384,397-419,429-451
Sequence
Target Sequence 
>C. ELEGANS:181239_S_AT
actctacggtccaacctatttgcgtgaagttttgaatttcgaagttcgcggaactggctt
cgctagtgctcttccattcttgctctcagcagctgtcaaatctatagccggacagctatc
agatcgctgtgattttgtatcagaacgggttagatttacaatttgcggaatcgttgcaag
actcggcttggcaatcggttatatcggaatggcaacgacgtcgagcaggctagtggctca
aattgcgttcactttttcgattgctgtatccggactgaacattatgggaacagtgaaatg
tctacagctgcgctgcaaacaacatgtgcactttgccgtctccgtcatcgcactcatggc
ctatgtgattcaattcggcgcgccaatcctcgtcggaatcatttgtccagataacaccgc
agaacagtggggatggttcttcttgatcgtcggaattattgtgttcgtcacaagtgcacc
ctttccatggtttacaactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTCTACGGTCCAACCTATTTGCGT 172 101 870 Antisense
GCGGAATCGTTGCAAGACTCGGCTT 656 293 1034 Antisense
GACTCGGCTTGGCAATCGGTTATAT 149 315 1049 Antisense
TGCGTTCACTTTTTCGATTGCTGTA 602 581 1113 Antisense
GTGAAATGTCTACAGCTGCGCTGCA 71 483 1162 Antisense
AACAACATGTGCACTTTGCCGTCTC 452 135 1187 Antisense
TCATCGCACTCATGGCCTATGTGAT 203 621 1214 Antisense
CCTATGTGATTCAATTCGGCGCGCC 167 265 1229 Antisense
CGCCAATCCTCGTCGGAATCATTTG 665 257 1250 Antisense
GATGGTTCTTCTTGATCGTCGGAAT 203 407 1301 Antisense
GCACCCTTTCCATGGTTTACAACTG 541 313 1345 Antisense
Sequence Source Affymetrix Proprietary Database